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  • Chemical Engineering  (20)
  • thema EDItEUR::N History and Archaeology::NH History  (14)
  • Saccharomyces cerevisiae
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  • 1
    Electronic Resource
    Electronic Resource
    Sequence and transcription of the β-glucosidase gene of Kluyveromyces fragilis cloned in Saccharomyces cerevisiae (1987)
    Springer
    Current genetics 12 (1987), S. 175-184 
    Details
    ISSN: 1432-0983
    Keywords: β-glucosidase ; Kluyveromyces fragilis ; DNA sequence ; Saccharomyces cerevisiae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The complete nucleotide sequence of the β-glucosidase gene of Kluyveromyces fragilis has been determined. This sequence contains an open reading frame of 2535 base pairs encoding a protein of 845 amino acids. Analysis of the transcription products revealed only one transcript of about 3 kb identical in both Kluyveromyces fragilis and in the expression host Saccharomyces cerevisiae. The protein molecular weight of 93,811 Kd deduced from the sequence is consistent with the 90,000 Kd determined by SDS polyacrylamide gel electrophoresis with the purified protein. Mapping of the starts of transcription shows that two starting points are used in the natural host Kluyveromyces fragilis. A comparison of the amino acid sequence with that of other β-glucosidases revealed three regions of homology. One of these regions contains an amino acid sequence very similar to a peptide isolated from the active site of β-glucosidase A3 from Aspergillus wentii and could be implicated in the catalytic mechanism of these glucolytic enzymes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00436876
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  • 2
    Electronic Resource
    Electronic Resource
    Construction of new yeast vectors and cloning of the nif (nitrogen fixation) gene cluster of Klebsiella pneumoniae in yeast (1981)
    Springer
    Current genetics 3 (1981), S. 173-180 
    Details
    ISSN: 1432-0983
    Keywords: Saccharomyces cerevisiae ; Yeast vectors ; Cosmids ; nif genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two vectors, termed pG63.11 (7.6 Kb) and pHCG3 (9.6 Kb), suitable for yeast transformation have been constructed. The pHCG3 vector has cosmid properties. Both vectors contain a single 3.3 Kb EcoRI-HindIII fragment of yeast origin which carries the yeast URA3 gene (1.1 Kb) and the origin of replication of the 2 µm plasmid (2.2 Kb). They confer ampicillin resistance and they contain 5 unique EcoRI,HpaI,HindIII,BamHI and SalI restriction sites. Cosmid pHCG3 was used to clone the nitrogen fixation (nif) gene cluster of Klebsiella pneumoniae carried by twoHindIII fragments of 17 and 26 Kb, respectively. The resulting cosmid, termed pGPC875 (53 Kb) which conferred a Nif+ phenotype to Escherichia coli, was introduced in yeast by transformation. No acetylene reduction activity was detectable in the transformants. However it was shown that the entire information for nitrogen fixation can be replicated and maintained intact in yeast for more than 50 generations of growth.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00429819
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  • 3
    Electronic Resource
    Electronic Resource
    The bI4 RNA mitochondrial maturase of Saccharomyces cerevisiae can stimulate intra-chromosomal recombination in Escherichia coli (1989)
    Springer
    Molecular genetics and genomics 216 (1989), S. 70-74 
    Details
    ISSN: 1617-4623
    Keywords: RNA splicing ; Maturase ; Recombinase ; Mitochondria ; Saccharomyces cerevisiae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary When the bI4 RNA maturase, encoded by the fourth intron of the mitochondrial cytochrome b gene of Saccharomyces cerevisiae, was expressed in Escherichia coli, formation of intra-chromosomal Lac+ recombinants was stimulated threefold. This “hyper-rec” phenotype was recA as well as recBCD dependent. The most active form of the bI4 maturase stimulated homologous recombination whereas splicing deficient mutants of bI4 maturase were either deficient in or unable to stimulate homologous recombination.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00332232
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  • 4
    Electronic Resource
    Electronic Resource
    PDR3, a new yeast regulatory gene, is homologous toPDR1 and controls the multidrug resistance phenomenon (1994)
    Springer
    Molecular genetics and genomics 244 (1994), S. 501-511 
    Details
    ISSN: 1617-4623
    Keywords: Saccharomyces cerevisiae ; Transcription factor ; Zinc finger ; Multidrug resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract TheSaccharomyces cerevisiae PDR3 gene, located near the centromere of chromosome II, has been completely sequenced and characterised. Mutationspdr3-1 andpdr3-2, which confer resistance to several antibiotics can be complemented by a wild-type allele of the PDR3 gene. The sequence of the wild-typePDR3 gene revealed the presence of a long open reading frame capable of encoding a 976-amino acid protein. The protein contains a single Zn(II)2Cys6 binuclear-type zinc finger homologous to the DNA-binding motifs of other transcriptional activators from lower eukaryotes. Evidence that the PDR3 protein is a transcriptional activator was provided by demonstrating that DNA-bound LexA-PDR3 fusion proteins stimulate expression of a nearby promoter containing LexA binding sites. The use of LexA-PDR3 fusions revealed that the protein contains two activation domains, one localised near the N-terminal, cysteine-rich domain and the other localised at the C-terminus. The salient feature of the PDR3 protein is its similarity to the protein coded byPDR1, a gene responsible forpleiotropicdrugresistance. The two proteins show 36% amino acid identity over their entire length and their zinc finger DNA-binding domains are highly conserved. The fact that the absence of both PDR1 and PDR3 (simultaneous disruption of the two genes) enhances multidrug sensitivity strongly suggests that the two transcriptional factors have closely related functions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00583901
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  • 5
    Electronic Resource
    Electronic Resource
    Dense-gas solvent-solute clusters at near-infinite dilution: EPR spectroscopic evidence (1993)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 39 (1993), S. 876-884 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The nitrogen hyperfine splitting constant of ditertbutyl nitroxide radicals was measured with electron paramagnetic resonance spectroscopy at near-infinite dilution in near-critical and supercritical ethane, as well as in liquid propane, liquid isobutane, and several nonhydrogen bonding liquid solvents. While the measurements in the liquids are described well by the theory of McRae, large deviations from the liquid behavior are observed in supercritical ethane. The deviations are used as a measure of the effective local density of the solvent around the solute. At the two temperatures investigated, Tr = 1.009 and Tr = 1.084, the local density enhancement, defined as the ratio of local to bulk densities, exhibits a maximum of about 3 occurring around 1/2 the critical density. The maximum is removed well from the critical density, where the maximum of the isothermal compressibility is observed. Local density enhancements are short-range effects and do not correlate well with the development of long-range critical phenomena. Local density enhancement data in ethane are compared with the prewetting transition that has been observed in nearcritical ultrapure argon.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690390516
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  • 6
    Electronic Resource
    Electronic Resource
    Prediction of pH in complex aqueous mixtures using a group-contribution method (1994)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 40 (1994), S. 1210-1222 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Expressions that suitably describe the behavior of weak electrolytes in aqueous systems are developed. A new numerical procedure is presented to allow for partial dissociation phenomena and so consider the whole species in solution. It is based on mass balances, the electroneutrality assumption, and dissociation constants of electrolytes. Combining it with a modified UNIFAC electrolyte group-contribution model based on the solvation concept directly allows calculation of pH, activities, and concentrations of species. The validity and usefulness of the expressions are tested using data for the pH of buffer solutions and complex aqueous mixtures such as fermentation media and liquid foods: pH values accurate to within ±0.05 pH units were obtained.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690400712
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  • 7
    Electronic Resource
    Electronic Resource
    Hydrodynamics of soil immobilization in the immobilized soil bioreactor (1997)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 43 (1997), S. 1163-1170 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The hydrodynamic characteristics of a new type of reactor, the immobilized soil bioreactor, were studied. This apparatus is a practical new engineering concept (soil immobilization) based on entrapment of soil particles, which contain pollutant-degrading microorganisms, in the pores of a geotextile to activate the indigenous microorganisms. The soil immobilization is the third on the size scale of immobilization processes, coming after (1) that of molecules in heterogeneous catalysis (in Angstrom) and (2) that of microbial cells and their fragments in immobilized cells and enzymes biocatalysis (in micron). The size of immobilized soil particles is in the range of a millimeter. A mathematical model of liquid flow within the reactor is proposed, which qualitatively explains the distribution of the immobilized soil in space. The dynamics of soil immobilization within the bioreactor has been studied as a function of the particle size, initial slurry concentraion and air flow rate. A mathematical model of the process of soil immobilization was proposed based on deep filter mechanics. The process can be described by a second-order kinetic model. This study will be of great importance for the design of immobilized soil bioreactors for degradation of recalcitrant soil pollutants.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690430505
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  • 8
    Electronic Resource
    Electronic Resource
    Quasicritical behavior of dense-gas solventsolute clusters at near-infinite dilution (1993)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 39 (1993), S. 1061-1071 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: We Compare molecular dynamics and electron paramagnetic resonance spectroscopic experiments on very dilute solutions of nitroxide free radicals in nearcritical and supercritical ethane. The measurement of the effective local density of solvent in the cybotactic region of the probe solute shows that maxima in local density enhancements occur in both computer and spectroscopic experiments at about half of the critical density, well removed from the maximum in the solvent's isothermal compressibility.Remarkably, at the bulk density where the maximum enhancement occurs, we observed long-lived clusters in the vicinity of the solute molecule. Geometrically defined clusters persist for at least 100 picoseconds of simulated time. The persistence of these clusters is corroborated by the behavior of the solute diffusion coefficient, which shows fluctuating values over simulated time periods as long as 1.3 nanoseconds. We argue that fluctuations in the local solvent density about a solute molecule are not a long-ranged solvent critical effect. Rather, they represent a localized, short-ranged quasicritical phenomenon induced by the solute, which may be described in terms of a transition between weakly attractive and attractive behavior. We discuss the implications of time-varying microstructure for reactions in supercritical fluid media.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690390615
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  • 9
    Electronic Resource
    Electronic Resource
    Some considerations and recalls on the design of gas absorbers: Laboratory apparatus to simulate absorber and to predict the effect of chemical reaction (1974)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 20 (1974), S. 1029-1031 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690200534
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  • 10
    Electronic Resource
    Electronic Resource
    Modified description of wave motion in a falling film (1966)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 12 (1966), S. 445-455 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The presence of gravity-capillary waves at the surface of a viscous falling film has been studied theoretically by a more rigorous linear treatment than presently available.The present analysis is based on the assumption of steady state periodic solutions of the complete Navier-Stokes equations. It provides methods of prediction, to a first order of approximation, for the wavelength, celerity, and wave number, in terms of the Weber number which emerges as the governing dimensionless group. This treatment includes as special cases the low Weber number analyses of Yih, Benjamin, Hanratty and Hershman, and Kapitza, as well as the high Weber number theory of Ishihara, Iwagaki, and Iwasa. Agreement with experimental observation is improved over that obtained from previous analyses.The stream function of the system has been derived and the instantaneous streamlines have been constructed for application to heat and mass transfer.
    Additional Material: 14 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690120311
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