Publication Date:
2001-11-27
Description:
Adhesions between fibroblastic cells and extracellular matrix have been studied extensively in vitro, but little is known about their in vivo counterparts. Here, we characterized the composition and function of adhesions in three-dimensional (3D) matrices derived from tissues or cell culture. "3D-matrix adhesions" differ from focal and fibrillar adhesions characterized on 2D substrates in their content of alpha5beta1 and alphavbeta3 integrins, paxillin, other cytoskeletal components, and tyrosine phosphorylation of focal adhesion kinase (FAK). Relative to 2D substrates, 3D-matrix interactions also display enhanced cell biological activities and narrowed integrin usage. These distinctive in vivo 3D-matrix adhesions differ in structure, localization, and function from classically described in vitro adhesions, and as such they may be more biologically relevant to living organisms.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Cukierman, E -- Pankov, R -- Stevens, D R -- Yamada, K M -- New York, N.Y. -- Science. 2001 Nov 23;294(5547):1708-12.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Craniofacial Developmental Biology and Regeneration Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11721053" target="_blank"〉PubMed〈/a〉
Keywords:
3T3 Cells
;
Animals
;
*Cell Adhesion/drug effects
;
Cell Culture Techniques/methods
;
Cell Division
;
Cell Movement
;
Cell Size
;
Cells, Cultured
;
Culture Techniques/methods
;
Cycloheximide/pharmacology
;
Cytoskeletal Proteins/metabolism
;
Extracellular Matrix/chemistry/metabolism
;
Fibroblasts/chemistry/*cytology/*metabolism
;
Fibronectins/metabolism
;
Fluorescent Antibody Technique, Indirect
;
Focal Adhesion Kinase 1
;
Focal Adhesion Protein-Tyrosine Kinases
;
Focal Adhesions/chemistry/metabolism
;
Glutaral/metabolism
;
Humans
;
Imaging, Three-Dimensional/*methods
;
Integrins/metabolism
;
Mice
;
Mitogen-Activated Protein Kinases/metabolism
;
Molecular Conformation
;
Phosphorylation
;
Protein-Tyrosine Kinases/metabolism
;
Time Factors
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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