ALBERT — All Library Books, journals and Electronic Records Telegrafenberg

Hits per page

hits 1 - 2 | 2 hits

Sorting

Electronic Resource

Cell wall degradation ofStaphylococcus aureus by lysozyme (1982)

Wecke, Jörg ; Lahav, Meir ; Ginsburg, Isaac ; [et al.]

Springer

Archives of microbiology 131 (1982), S. 116-123

add to mindlist on the mindlist

Details

ISSN: 1432-072X

Keywords: Cell wall ; Wall degradation ; Lysozyme ; Autolysines ; Electron microscopy ; Staphylococcus aureus

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract In contrast to former findings lysozyme was able to attack the cell walls ofStaphylococcus aureus under acid conditions. However, experiments with14C-labelled cell walls and ribonuclease indicated that, under these conditions, lysozyme acted less as an muralytic enzyme but more as an activator of pre-existing autolytic wall enzymes. Electron microscopic studies showed that under these acid conditions the cell walls were degraded by a new mechanism (i.e. “attack from the inside”). This attack on the cell wall started asymmetrically within the region of the cross wall and induced the formation of periodically arranged lytic sites between the cytoplasmic membrane and the cell wall proper. Subsequently, a gap between the cell wall and the cytoplasmic membrane resulted and large cell wall segments became detached and suspended in the medium. The sequence of lytic events corresponded to processes known to take place during wall regeneration and wall formation. In the final stage of lysozyme action at pH 5 no cell debris but “stabilized protoplasts” were to be seen without detectable alterations of the primary shape of the cells. At the same time long extended ribbon-like structures appeared outside the bacteria. The origin as well as the chemical nature of this material is discussed. Furthermore, immunological implications are considered.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF01053992

Permalink

	Location	Call Number	Expected	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

Electronic Resource

Growth and Dissolution of Organic Crystals with “Tailor-Made” Inhibitors - Implications in Stereochemistry and Materials ScienceIn memoriam Gerhard M. J. Schmidt (1985)

Addadi, Lia ; Berkovitch-Yellin, Ziva ; Weissbuch, Isabelle ; [et al.]

Weinheim : Wiley-Blackwell

Angewandte Chemie International Edition in English 24 (1985), S. 466-485

add to mindlist on the mindlist

Details

ISSN: 0570-0833

Keywords: Crystalline polymers ; Inhibitors ; Stereochemistry ; Materials science ; Morphology ; Crystal morphology ; Chemistry ; General Chemistry

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology

Notes: When crystals of organic compounds are grown in the presence of growth inhibitors, there is a change in crystal morphology. A stereochemical correlation exists between the crystal structure, its modified morphology, and the molecular structure of the inhibitor. This correlation has been successfully exploited for the efficient resolution of conglomerates, the engineering of organic crystals with desired morphologies, the direct and relative assignment of the absolute configurations of chiral molecules and crystals, and for the design of a new model for the spontaneous generation of optical activity. In an analogous way dissolution of organic crystals in the presence of these growth inhibitors induces etch pits at preselected faces. The effect of solvent on crystal growth has been analyzed in some model systems. The experimental results are complemented by atom-atom potential energy calculations.

Additional Material: 31 Ill.