Publication Date:
1985-07-19
Description:
In experiments designed to study the mechanism by which peptide hormones binding to their plasma membrane receptors stimulate the expression of specific genes, the transcription of two neuroendocrine genes, prolactin and growth hormone, was analyzed in a rat pituitary cell line. The results showed that cyclic adenosine monophosphate (cyclic AMP) stimulates the transcription of discrete subsets of eukaryotic genes by at least two independent molecular mechanisms. Cyclic AMP stimulated growth hormone gene transcription and phosphorylation of a 19,000-dalton nuclear protein; this appears to reflect direct nuclear actions of the cyclic AMP-dependent protein kinase. In contrast, the stimulation by cyclic AMP of prolactin gene transcription appears to reflect activation of a discrete calcium-dependent event.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Waterman, M -- Murdoch, G H -- Evans, R M -- Rosenfeld, M G -- New York, N.Y. -- Science. 1985 Jul 19;229(4710):267-9.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2990047" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Cell Line
;
Cell Membrane/metabolism
;
Cobalt/pharmacology
;
Colforsin
;
Cyclic AMP/*physiology
;
Diterpenes/pharmacology
;
Growth Hormone/biosynthesis/genetics
;
Phosphorylation
;
Pituitary Gland/cytology
;
Prolactin/biosynthesis/genetics
;
Protein Kinases/physiology
;
Rats
;
Thyrotropin-Releasing Hormone/pharmacology
;
*Transcription, Genetic/drug effects
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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