Publication Date:
2011-11-26
Description:
B-type lamins, the major components of the nuclear lamina, are believed to be essential for cell proliferation and survival. We found that mouse embryonic stem cells (ESCs) do not need any lamins for self-renewal and pluripotency. Although genome-wide lamin-B binding profiles correlate with reduced gene expression, such binding is not directly required for gene silencing in ESCs or trophectoderm cells. However, B-type lamins are required for proper organogenesis. Defects in spindle orientation in neural progenitor cells and migration of neurons probably cause brain disorganizations found in lamin-B null mice. Thus, our studies not only disprove several prevailing views of lamin-Bs but also establish a foundation for redefining the function of the nuclear lamina in the context of tissue building and homeostasis.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3306219/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3306219/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kim, Youngjo -- Sharov, Alexei A -- McDole, Katie -- Cheng, Melody -- Hao, Haiping -- Fan, Chen-Ming -- Gaiano, Nicholas -- Ko, Minoru S H -- Zheng, Yixian -- R01 AR060042/AR/NIAMS NIH HHS/ -- R01 AR060042-02/AR/NIAMS NIH HHS/ -- R01 GM 56312/GM/NIGMS NIH HHS/ -- Howard Hughes Medical Institute/ -- Intramural NIH HHS/ -- New York, N.Y. -- Science. 2011 Dec 23;334(6063):1706-10. doi: 10.1126/science.1211222. Epub 2011 Nov 24.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Embryology, Carnegie Institution for Science, Baltimore, MD 21218, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/22116031" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Body Size
;
Brain/cytology/embryology
;
Cell Cycle
;
Cell Differentiation
;
Cell Movement
;
Cells, Cultured
;
Chromatin/metabolism
;
Embryonic Development
;
Embryonic Stem Cells/cytology/*physiology
;
Female
;
Gene Expression Regulation, Developmental
;
Gene Silencing
;
Lamin Type B/genetics/metabolism/*physiology
;
Male
;
Mice
;
Mice, Knockout
;
Neural Stem Cells/cytology
;
Neurons/cytology
;
Nuclear Lamina/physiology
;
Organ Size
;
*Organogenesis
;
Pluripotent Stem Cells/cytology/physiology
;
Promoter Regions, Genetic
;
Spindle Apparatus/physiology/ultrastructure
;
Transcription, Genetic
;
Trophoblasts/cytology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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