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  • Cell Analyzer  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Time lapse records of cells in vitro using optical memory disk and cell analyzer (1988)
    Springer
    Methods in cell science 11 (1988), S. 19-22 
    Details
    ISSN: 1573-0603
    Keywords: time lapse ; optical disk ; image recording ; Cell Analyzer ; image cytometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A system has been developed for making automated cinematographic records, using the Cell Analyzer imaging apparatus and an optical memory disk recorder (OMDR). The system is particularly useful when numerous individual cells must be followed over an extended period in a single experiment where the cells are growing in a multichamber tissue culture vessel, or are sparsely distributed within a single flask. The system is programmed to revisit each cell location at a prescribed time interval and to record a video frame on the OMDR. Each frame is stored on a preselected position on the optical memory disk under computer control so that individual records of each cell can be played back in sequence at a selected speed immediately after the experiment. The system can also make use of the Cell Analyzer imaging functions, such as the tracking of cell positions and quantitative measurements of morphologic cell features.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01404409
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  • 2
    Electronic Resource
    Electronic Resource
    3T3 cell motility in the temperature range 33°C to 39°C (1987)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 7 (1987), S. 361-367 
    Details
    ISSN: 0886-1544
    Keywords: Cell Analyzer ; cell motility ; temperature ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The phenomena of mammalian cell motility in tissue culture is an integrated function of many cellular components. As such, cell motility is very sensitive to external stimuli and perturbation. In this article we report the effect of temperature in the range 33°C to 39°C on cell motility. For this 3T3 cells were plated in plastic tissue culture flasks. A large number of individual cells (60 per experiment) were tracked as a function of time by means of an automated device, the Cell Analyzer. The data show a peak in the average cell speed in the range 36.5°C to 38.5°C, falling off sharply at lower and higher temperatures. The average rate of cell motility closely correlates to the average cell proliferation rate in the range 33°C to 39°C.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cm.970070408
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