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Defect in polyamine metabolism in a BHK cell mutant temperature-sensitive for rRNA maturation (1979)

Levin, Eugene G. ; Clark, Jeffrey L.

New York, NY [u.a.] : Wiley-Blackwell

Journal of Cellular Physiology 101 (1979), S. 361-368

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ISSN: 0021-9541

Keywords: Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Medicine

Notes: A mutant of BHK cells (ts422E) temperature-sensitive for processing 32S rRNA to 28S rRNA (Toniolo et al., '73) also loses the ability to synthesize polyamines and 5.8S rRNA when shifted to the non-permissive temperature (39°). The activity of several enzymes not involved with polyamine synthesis, methylation of 32S rRNA, and small nuclear RNA production are apparently unaffected after at least 24 hours at 39°. When cultures are returned to the permissive temperature (33°), polyamine synthesizing capacity returns to normal as mature rRNA production resumes.

Additional Material: 5 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/jcp.1041010303

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