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  • Articles  (2)
  • Cell & Developmental Biology  (1)
  • somatic cell genetics  (1)
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  • Articles  (2)
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  • 1
    Electronic Resource
    Electronic Resource
    Bioautography: A general method for the visualization of isozymes (1977)
    Springer
    Biochemical genetics 15 (1977), S. 1193-1211 
    Details
    ISSN: 1573-4927
    Keywords: isozymes ; biological assay ; somatic cell genetics ; electrophoresis ; histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A new method has been developed for visualization of isozymes which are difficult or impossible to detect with standard histochemical or autoradiographic methods. The principle of this method, bioautography, is the use of a microbial reagent to locate an enzyme after gel electrophoresis. When bioautography was compared to other staining procedures, the bioautographic method yielded identical results to those observed by the histochemical method for lactate dehydrogenase (LDH) or by the autoradiographic method for the adenine phosphoribosyl-transferase (APRT). Using the bioautographic method, stains for enzymes which could not be visualized by any other procedure have been developed: argininosuccinate lyase and branched-chain aminotransferase. By employing appropriately genetically marked bacterial strains, it should be possible to develop new isozyme stains for a large number of unstudied isozymes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00484509
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  • 2
    Electronic Resource
    Electronic Resource
    Structure and chromosomal localization of the human lymphotoxin gene (1985)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 29 (1985), S. 171-181 
    Details
    ISSN: 0730-2312
    Keywords: lymphotoxin ; cytotoxic factor ; gene structure ; chromosome 6 ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: We have isolated, sequenced, and determined the chromosomal localization of the gene encoding human lymphotoxin (LT). The single copy gene was isolated from a human genomic library using a 32P-labeled 116 bp synthetic DNA fragment whose sequence was based on the NH2-terminal amino acid sequence of LT. The gene spans 3 kb of DNA and is interrupted by three intervening sequences. The LT gene is located on human chromosome 6, as determined by Southern blot analysis of human-murine hybrid DNA. Putative transcriptional control regions and areas of homology with the promoters of interferon and other genes are identified.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcb.240290302
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