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  • 1
    ISSN: 1573-5036
    Keywords: durum wheat ; leaf area ratio ; net assimilation rate ; developmental plasticity ; moisture stress ; relative growth rate ; shoot:root ratio
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Understanding how growth and development of durum wheat cultivars respond to drought could provide a basis to develop crop improvement programmes in drought-affected tropical and subtropical countries. A greenhouse experiment was conducted to study the responses of five durum wheat cultivars to moisture stress at different developmental phases. Phenology, total dry matter (TDM), relative growth rate (RGR), leaf area ratio (LAR), net assimilation rate (NAR), leaf weight ratio (LWR), specific leaf area (SLA) and shoot:root ratio were compared. Pre-anthesis moisture stress delayed phenological development, whereas post-anthesis moisture stress accelerated it. TDM accumulation rate was different between drought-resistant and susceptible cultivars. RGR and its components changed with age and moisture availability. Drought-resistant cultivars had a high RGR in favourable periods of the growing season and a low RGR during moisture stress. In contrast, the drought-susceptible cultivar (Po) showed an opposite trend. LAR explained the differences in RGR (r=0.788) best, whereas the relationship between NAR and RGR was not significant. Even though both LWR and SLA were important factors determining the potential growth rate, LWR was of major importance to describe cultivar differences in LAR, and consequently in RGR. The drought-resistant cultivars Omrabi-5 and Boohai showed vigorous root development and/or a low shoot:root ratio. It is concluded that biomass allocation is the major factor explaining variation in RGR among the investigated durum wheat cultivars.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-5060
    Keywords: drought susceptibility index ; moisture stress ; path analysis ; Triticum turgidum var. durum ; yield components ; yield potential
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary One main reason for the slow improvement of durum wheat in water-limited environments is the lack of clear understanding of the interrelationships among yield components and their compensatory changes under low and erratic moisture availability. Five cultivars, varying in many physiological attributes, were tested under different drought-stress conditions in field and greenhouse experiments. The cause-effect relationships of duration of vegetative period, duration of grain-filling period, number of spikes per m2, kernels per spike, kernel weight and grain yield per m2 were assessed. Furthermore, yield stability was evaluated. Yield reduction was largest under mid-season stress (58%), followed by terminal stress (30%) and early stress (22%). Cultivar Po was very sensitive to terminal stress. Path-coefficient analysis revealed a complex pattern of relationships among the six variables. An increase in vegetative period reduced the grain-filling period under all conditions. It increased number of kernels per spike under non-stress conditions. The direct effect of spikes per m2 on grain yield was significantly positive. However, more spikes per m2 resulted in fewer kernels per spike and a low kernel weight and, as a result, a negative relationship with grain yield under early stress. Grain-filling period had a strong influence on grain yield via kernel weight. Kernels per spike had the largest direct effect on grain yield. However, it was negatively correlated with kernel weight, especially under terminal stress. Grain yield heavily depended on kernels per spike under early stress and grain-filling period and kernels per spike under terminal stress. Variation in drought susceptibility index among cultivars was significant under early and terminal stress conditions, but not under mid-stress conditions. Yield potential and stability were not correlated for the different drought-stress conditions. Longer grain-filling period, increased number of kernels per spike and limited spike number per m2 can be used as selection criteria for sustainable yield in water-limited environments.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 77 (1971), S. 337-352 
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Intracellular microelectrode studies of passive membrane properties and action potential generation were carried out on cloned and uncloned mouse neuroblastoma cells in tissue culture. The cloned cells were studied between the eighth and tenth months and the uncloned cells between the third and fifth weeks after primary dissociation. Electrophysiologic measurements of cell membrane properties were made by passing stimulating current pulses across the cell membrane from an intracellular microelectrode and recording simultaneously from the same electrode, by means of a bridge circuit, the changes in membrane potential. The range of responses to electrical stimulation varied from passive increases in membrane potential to repetitive firing of action potentials. A 20 fold range in spike generating capability was found. Passive membrane properties (membrane potential, specific membrane resistivity, and specific membrane capacitance) were similar to those of sympathetic neurons in intact preparations. Seventy-nine percent of the cloned cell line compared to 94% of the uncloned line were capable of generating action potentials. Less than 2% of the cloned cells showed repetitive firing whereas 23% of the uncloned cells had this property. As in several types of normal neurons, the action potential mechanism was largely, although not completely, blocked by iontophoretic and bath applied tetrodotoxin.
    Additional Material: 7 Ill.
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  • 4
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 77 (1971), S. 353-362 
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Dissociated mouse neuroblastoma cells were studied in vitro by using intracellular microelectrodes for electrical stimulation and recording. Some, but not all cells, which exhibited well developed action potentials to electrical stimulation also showed changes in membrane potential to iontophoretically applied acetylcholine (ACh). The types of responses to ACh varied. Short latency depolarizing responses to pulses of ACh (similar to those obtained with skeletal muscle) as well as sustained depolarization to steady ACh application (D response) occurred. A longer latency prolonged hyperpolarizing response (H response) and bi- and triphasic combinations of H and D responses were also seen.Pairs of cells showing morphologic contact were tested for the occurrence of effective synaptic coupling by placing intracellular microelectrodes in each cell. In none of 95 cases tested did spike activity produced by direct electrical stimulation of one cell elicit a synaptic potential of 200 μv or more in the other.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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