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  • 1
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Journal of Morphology 192 (1987), S. 27-42 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The fate and possible roles of the cytoskeleton in the process of conjugation in the hyptrich ciliate Euplotes aediculatus were investigated. Following the coalescence of the plasma membranes of the conjugant cells, a fusion zone or bridge of cytoplasm contributed by both partners is constructed. The sub-alveolar microtubule layers of the vegetative cell cortex remain in place to define the fusion zone boundaries after cell union. The initial fusion zone consists primarily of featureless ground cytoplasm; soon the ground plasm becomes crowded with microtubules and anastomosing smooth endoplasmic reticulum, which become displaced only late in conjugation as the migratory pronuclei are exchanged between partners. Fusion zone microtubules, functioning in some undetermined way, may be involved in the nuclear migration. Resorption of the posterior portion of each partner's buccal apparatus results in the degradation of the component cilia within acid phosphatase-positive autophagic bodies. Silver staining for light microscopy shows that the late fusion zone contracts forward from the posterior border, then constricts to separate the conjugants. In some separating pairs remnants of a microfilamentous assembly are seen at the posterior edge of the fusion zone; the full extent of this system may be masked by partial degradation due to osmium tetroxide fixation. Treatment of conjugants for 6 hours with cytochalasin B prevents separation, possibly through inhibition of the actin-like microfilament assembly in the fusion zone. The observations and experiments favor a model of cell separation following conjugation in which the fusion zone is resorbed by motile or contractile processes occurring within or around the fusion bridge itself.
    Additional Material: 21 Ill.
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Journal of Morphology 192 (1987), S. 43-61 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The formation and subsequent dissolution of a common bridge of cytoplasm between conjugating ciliated protozoan cells provides an excellent opportunity to follow the dynamics of the cellular membrane systems involved in this process. In particular, separation of conjugant partners offers the chance to observe, at a fixed site on the cell surface, how the ciliate surface complex of plasma and alveolar membranes (collectively termed the “pellicle”) is constructed. Consequently, cortical and cellular membranes of Euplotes aediculatus were studied by light and electron microscopy through the conjugation sequence. A conjugant fusion zone of shared cytoplasm elaborates between the partner cells within their respective oral fields (peristomes) to include microtubules, cytosol, and a concentrated endoplasmic reticulum (heavily stained by osmium impregnation techniques) that may also be continuous with cortical ER of each cell. Cortical membranes displacd by fusion are autolyzed in acid phosphatase-positive lysosomes in the fusion zone. As conjugants separate, expansion of the plasma membrane may occur through the fusion of vesicles with the plasma membrane, presumably at bare membrane, presumably at bare membrane patches near the fusion zone. The underlying cortical alveolar membranes and their plate-like contents are reconstructed beneath the plasma membrane, apparently by multiple fusions of dense-cored alveolar precursor vesicles (APVs). These precursor vesicles themselves appear to condense directly from the smooth ER present in the fusion zone. No Golgi apparatus was visible in the fusion zone cytoplasm, and no step of APV maturation that might involve the Golgi complex was noted.
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  • 3
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 124 (1985), S. 391-396 
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The addition of human platelet-derived growth factor (PDGF) to confluent, quiescent cultures of human diploid fibroblasts induced the rapid breakdown of cellular polyphosphoinositides. The levels of 32P-labeled phosphatidylinositol 4,5-bisphosphate (PIP2), phosphatidylinositol 4-phosphate (PIP), and phosphatidylinositol (PI) decreased by 30 to 40% within 1 min after exposure of the cells to PDGF. The levels of PIP and PIP2 returned to their initial values within 3 and 10 min, respectively, after PDGF addition. The level of PI continued to increase after it had returned to control values and was up threefold within 30 min after PDGF addition. In cells prelabeled with myo-[3H]inositol PDGF caused an eightfold increase in the levels of inositol trisphosphate (IP3) within 2 min. Lesser increases, twofold and 1.3-fold, respectively, were seen in levels of inositol bisphosphate (IP2) and inositol monophosphate (IP). Within 10 min after PDGF addition the levels of all three inositol phosphates had decreased to control values. The levels of IP3 measured 2 min after PDGF addition depended on the PDGF concentration and were maximal at 5-10 ng/ml of PDGF. Similar concentrations of PDGF stimulate maximal cell growth and DNA synthesis in these cells.
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  • 4
    Publication Date: 2022-05-25
    Description: Author Posting. © American Meteorological Society, 2018. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in he balance of salinity variance in a partially stratified estuary: Implications for exchange flow, mixing, and stratification. Journal of Physical Oceanography, 48(12), (2018) 2887-2899., doi: 10.1175/JPO-D-18-0032.1.
    Description: Salinity variance dissipation is related to exchange flow through the salinity variance balance equation, and meanwhile its magnitude is also proportional to the turbulence production and stratification inside the estuary. As river flow increases, estuarine volume-integrated salinity variance dissipation increases owing to more variance input from the open boundaries driven by exchange flow and river flow. This corresponds to the increased efficient conversion of turbulence production to salinity variance dissipation due to the intensified stratification with higher river flow. Through the spring–neap cycle, the temporal variation of salinity variance dissipation is more dependent on stratification than turbulence production, so it reaches its maximum during the transition from neap to spring tides. During most of the transition time from spring to neap tides, the advective input of salinity variance from the open boundaries is larger than dissipation, resulting in the net increase of variance, which is mainly expressed as vertical variance, that is, stratification. The intensified stratification in turn increases salinity variance dissipation. During neap tides, a large amount of enhanced salinity variance dissipation is induced by the internal shear stress near the halocline. During most of the transition time from neap to spring tides, dissipation becomes larger than the advective input, so salinity variance decreases and the stratification is destroyed.
    Description: TW was supported by the National Key R&D Program of China (Grant 2017YFA0604104), National Natural Science Foundation of China (Grant 41706002), Natural Science Foundation of Jiangsu Province (Grant BK20170864), and MEL Visiting Fellowship (MELRS1617). WRG was supported by NSF Grant OCE 1736539. Part of this work is finished during TW’s visit in MEL and WHOI. We would like to acknowledge John Warner for providing the codes of the Hudson estuary model, and Parker MacCready, the editor, and two reviewers for their insightful suggestions on improving the manuscript.
    Description: 2019-06-06
    Keywords: Estuaries ; Dynamics ; Mixing ; Density Currents
    Repository Name: Woods Hole Open Access Server
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  • 5
    Publication Date: 2022-05-25
    Description: Author Posting. © American Meteorological Society, 2007. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 37 (2007): 1859-1877, doi:10.1175/jpo3088.1.
    Description: A series of dye releases in the Hudson River estuary elucidated diapycnal mixing rates and temporal variability over tidal and fortnightly time scales. Dye was injected in the bottom boundary layer for each of four releases during different phases of the tide and of the spring–neap cycle. Diapycnal mixing occurs primarily through entrainment that is driven by shear production in the bottom boundary layer. On flood the dye extended vertically through the bottom mixed layer, and its concentration decreased abruptly near the base of the pycnocline, usually at a height corresponding to a velocity maximum. Boundary layer growth is consistent with a one-dimensional, stress-driven entrainment model. A model was developed for the vertical structure of the vertical eddy viscosity in the flood tide boundary layer that is proportional to u2*/N∞, where u* and N∞ are the bottom friction velocity and buoyancy frequency above the boundary layer. The model also predicts that the buoyancy flux averaged over the bottom boundary layer is equal to 0.06N∞u2* or, based on the structure of the boundary layer equal to 0.1NBLu2*, where NBL is the buoyancy frequency across the flood-tide boundary layer. Estimates of shear production and buoyancy flux indicate that the flux Richardson number in the flood-tide boundary layer is 0.1–0.18, consistent with the model indicating that the flux Richardson number is between 0.1 and 0.14. During ebb, the boundary layer was more stratified, and its vertical extent was not as sharply delineated as in the flood. During neap tide the rate of mixing during ebb was significantly weaker than on flood, owing to reduced bottom stress and stabilization by stratification. As tidal amplitude increased ebb mixing increased and more closely resembled the boundary layer entrainment process observed during the flood. Tidal straining modestly increased the entrainment rate during the flood, and it restratified the boundary layer and inhibited mixing during the ebb.
    Description: The work was supported by the National Science Foundation Grant OCE00-95972 (W. Geyer, J. Lerczak), OCE00-99310 (R. Houghton), and OCE00-95913 (R. Chant, E. Hunter).
    Keywords: Estuaries ; Boundary layer ; Mixing ; Tides ; Friction
    Repository Name: Woods Hole Open Access Server
    Type: Article
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  • 6
    Publication Date: 2022-05-25
    Description: © The Author(s), 2014. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Geophysical Research Letters 41 (2014): 8987–8993, doi:10.1002/2014GL062274.
    Description: Observations at the Columbia River plume show that wave breaking is an important source of turbulence at the offshore front, which may contribute to plume mixing. The lateral gradient of current associated with the plume front is sufficient to block (and break) shorter waves. The intense whitecapping that then occurs at the front is a significant source of turbulence, which diffuses downward from the surface according to a scaling determined by the wave height and the gradient of wave energy flux. This process is distinct from the shear-driven mixing that occurs at the interface of river water and ocean water. Observations with and without short waves are examined, especially in two cases in which the background conditions (i.e., tidal flows and river discharge) are otherwise identical.
    Description: This work was supported by the Office of Naval Research, as part of the Data Assimilation and Remote Sensing for Littoral Applications (DARLA) project and in coordination with the Rivers and Inlets (RIVET) program.
    Keywords: Wave breaking ; Turbulence ; Mixing ; Wave-current interaction ; River plume
    Repository Name: Woods Hole Open Access Server
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  • 7
    Publication Date: 2022-05-25
    Description: Author Posting. © American Geophysical Union, 2017. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research: Oceans 122 (2017): 4743–4760, doi:10.1002/2016JC012455.
    Description: Estuarine mixing is often intensified in regions where topographic forcing leads to hydraulic transitions. Observations in the salt-wedge estuary of the Connecticut River indicate that intense mixing occurs during the ebb tide in regions of supercritical flow that is accelerated by lateral expansion of the channel. The zones of mixing are readily identifiable based on echo-sounding images of large-amplitude shear instabilities. The gradient Richardson number (Ri) averaged across the mixing layer decreases to a value very close to 0.25 during most of the active mixing phase. The along-estuary variation in internal Froude number and interface elevation are roughly consistent with a steady, inviscid, two-layer hydraulic representation, and the fit is improved when a parameterization for interfacial stress is included. The analysis indicates that the mixing results from lateral straining of the shear layer, and that the rapid development of instabilities maintains the overall flow near the mixing threshold value of Ri = 0.25, even with continuous, active mixing. The entrainment coefficient can be estimated from salt conservation within the interfacial layer, based on the finding that the mixing maintains Ri = 0.25. This approach leads to a scaling estimate for the interfacial mixing coefficient based on the lateral spreading rate and the aspect ratio of the flow, yielding estimates of turbulent dissipation within the pycnocline that are consistent with estimates based on turbulence-resolving measurements.
    Description: NSF Grant Number: OCE 0926427; Devonshire Scholars program
    Description: 2017-12-12
    Keywords: Internal hydraulics ; Mixing ; Gradient Richardson number ; Estuary
    Repository Name: Woods Hole Open Access Server
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  • 8
    Publication Date: 2022-05-25
    Description: Author Posting. © American Meteorological Society, 2018. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 48 (2018): 607-623, doi:10.1175/JPO-D-17-0189.1.
    Description: The roles of straining and dissipation in controlling stratification are derived analytically using a vertical salinity variance method. Stratification is produced by converting horizontal variance to vertical variance via straining, that is, differential advection of horizontal salinity gradients, and stratification is destroyed by the dissipation of vertical variance through turbulent mixing. A numerical model is applied to the Changjiang estuary in order to demonstrate the salinity variance balance and how it reveals the factors controlling stratification. The variance analysis reveals that dissipation reaches its maximum during spring tide in the Changjiang estuary, leading to the lowest stratification. Stratification increases from spring tide to neap tide because of the increasing excess of straining over dissipation. Throughout the spring–neap tidal cycle, straining is almost always larger than dissipation, indicating a net excess of production of vertical variance relative to dissipation. This excess is balanced on average by advection, which exports vertical variance out of the estuarine region into the plume. During neap tide, tidal straining shows a general tendency of destratification during the flood tide and restratification during ebb, consistent with the one-dimensional theory of tidal straining. During spring tide, however, positive straining occurs during flood because of the strong baroclinicity induced by the intensified horizontal salinity gradient. These results indicate that the salinity variance method provides a valuable approach for examining the spatial and temporal variability of stratification in estuaries and coastal environments.
    Description: X. Li was supported by the China Scholarship Council. W. R. Geyer was supported by NSF Grants OCE 1736539 and OCE 1634480. J. Zhu was supported by the National Natural Science Foundation of China (41476077 and 41676083). H. Wu was supported by the National Natural Science Foundation of China (41576088 and 41776101).
    Description: 2018-09-08
    Keywords: Ocean ; Estuaries ; Freshwater ; Mixing ; Numerical analysis/modeling ; Regional models
    Repository Name: Woods Hole Open Access Server
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  • 9
    Publication Date: 2022-05-25
    Description: Author Posting. © American Geophysical Union, 2008. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research 113 (2008): C07023, doi:10.1029/2007JC004644.
    Description: A sequence of dye releases in the Hudson River estuary provide a quantitative assessment of horizontal dispersion in a partially mixed estuary. Dye was released in the bottom boundary layer on 4 separate occasions, with varying tidal phase and spring-neap conditions. The three-dimensional distribution of dye was monitored by two vessels with in situ, profiling fluorometers. The three-dimensional spreading of the dye was estimated by calculating the time derivative of the second moment of the dye in the along-estuary, cross-estuary and vertical directions. The average along-estuary dispersion rate was about 100 m2/s, but maximum rates up to 700 m2/s occurred during ebb tides, and minimum rates occurred during flood. Vertical shear dispersion was the principal mechanism during neap tides, but transverse shear dispersion became more important during springs. Suppression of mixing across the pycnocline limited the vertical extent of the patch in all but the maximum spring-tide conditions, with vertical diffusivities in the pycnocline estimated at 4 × 10−5 m2/s during neaps. The limited vertical extent of the dye patch limited the dispersion of the dye relative to the overall estuarine dispersion rate, which was an order of magnitude greater than that of the dye. This study indicates that the effective dispersion of waterborne material in an estuary depends sensitively on its vertical distribution as well as the phase of the spring-neap cycle.
    Description: This research was supported by National Science Foundation Grant OCE04-52054 (W. Geyer), OCE00-99310 (R. Houghton), and OCE00-95913 (R. Chant).
    Keywords: Dispersion ; Mixing ; Spring-neap variations
    Repository Name: Woods Hole Open Access Server
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  • 10
    Publication Date: 2022-05-25
    Description: Author Posting. © American Meteorological Society, 2008. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 38 (2008): 418-434, doi:10.1175/2007JPO3372.1.
    Description: Stratification and turbulent mixing exhibit a flood–ebb tidal asymmetry in estuaries and continental shelf regions affected by horizontal density gradients. The authors use a large-eddy simulation (LES) model to investigate the penetration of a tidally driven bottom boundary layer into stratified water in the presence of a horizontal density gradient. Turbulence in the bottom boundary layer is driven by bottom stress during flood tides, with low-gradient (Ri) and flux (Rf) Richardson numbers, but by localized shear during ebb tides, with Ri = ¼ and Rf = 0.2 in the upper half of the boundary layer. If the water column is unstratified initially, the LES model reproduces periodic stratification associated with tidal straining. The model results show that the energetics criterion based on the competition between tidal straining and tidal stirring provides a good prediction for the onset of periodic stratification, but the tidally averaged horizontal Richardson number Rix has a threshold value of about 0.2, which is lower than the 3 suggested in a recent study. Although the tidal straining leads to negative buoyancy flux on flood tides, the authors find that for typical values of the horizontal density gradient and tidal currents in estuaries and shelf regions, buoyancy production is much smaller than shear production in generating turbulent kinetic energy.
    Description: This work is supported by Grants OCE-0451699 and OCE-0451740 from the National Science Foundation.
    Keywords: Tides ; Mixing ; Large eddy simulations
    Repository Name: Woods Hole Open Access Server
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