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  • 1
    Electronic Resource
    Electronic Resource
    Nocodazole pretreatment in anaphase selectively reduces anaphase B in PtK1 cells (1983)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 3 (1983), S. 79-91 
    Details
    ISSN: 0886-1544
    Keywords: mitosis ; anaphase ; microtubules ; nocodazole ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: During early anaphase PtK1 cells were briefly treated with the rapidly reversible microtubule (MT) poison nocodazole. This treatment abruptly stopped chromosome motion and effected a large decrease in spindle birefringence. On removal of the drug, chromosome to pole motion (anaphase A) returned, though at a lesser rate but not extent than untreated cells. In most cases elongation of the pole-pole distance (anaphase B) also occured, at both a rate and to an extent less than in untreated cells. During the recovery period following drug arrest spindle birefringence did not return to pretreatment levels. Electron microscopic analysis of nocodazole arrested, or arrested and released, cells revealed extensive disassembly of the nonkinetochore class of MTs (nkMTs), particularly evident in the astral region. Microtubules seen in the interzone region were largely fragments of midbody precursors. Kinetochore MTs (kMTs) appeared to be unaffected by the brief drug treatment chosen for these experiments. Analysis of MT profiles seen in transverse sections of the interzone region indicated in treated and released cells approximately 60% fewer MTs. This may suggest that chromosome motion during anaphase is not dependent on interactions between kMTs and nkMTs and separation of the spindle poles can occur in the presence of disrupted interzonal MTs.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cm.970030107
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  • 2
    Electronic Resource
    Electronic Resource
    Effect of chronic isoproterenol exposure on insulin binding and insulin-stimulated hexose transport in isolated rat adipocytes
    Amsterdam : Elsevier
    Biochemical and Biophysical Research Communications 148 (1987), S. 1093-1097 
    Details
    ISSN: 0006-291X
    Keywords: [abr] BSA; bovine serum albumin ; [abr] HEPES; N-2-hydroxyethyl-piperazine-N'-2-ethane sulfonicacid ; [abr] KRH; Kreb's Ringer HEPES buffer
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/S0006-291X(87)80244-9
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  • 3
    Electronic Resource
    Electronic Resource
    The determination of non-steroidal antiinflammatory drugs in pharmaceuticals by capillary zone electrophoresis and micellar electrokinetic capillary chromatography
    Amsterdam : Elsevier
    Journal of Pharmaceutical and Biomedical Analysis 12 (1994), S. 21-26 
    Details
    ISSN: 0731-7085
    Keywords: MEKC. ; NSAIDs ; capillary electrophoresis ; pharmaceutical formulations
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology , Medicine
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0731-7085(94)80005-7
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  • 4
    Electronic Resource
    Electronic Resource
    Capillary zone electrophoresis and micellar electrokinetic capillary chromatography of some nonsteroidal antiinflammatory drugs (NSAIDs)
    Amsterdam : Elsevier
    Journal of Pharmaceutical and Biomedical Analysis 11 (1993), S. 197-201 
    Details
    ISSN: 0731-7085
    Keywords: Non-steroidal antiinflammatory drugs ; capillary electrophoresis ; micellar electrokinetic capillary chromatography.
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology , Medicine
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0731-7085(93)80196-8
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  • 5
    Electronic Resource
    Electronic Resource
    Effect of chronic isoproterenol exposure on insulin binding and insulin-stimulated hexose transport in isolated rat adipocytes
    Amsterdam : Elsevier
    Biochemical and Biophysical Research Communications 148 (1987), S. 1093-1097 
    Details
    ISSN: 0006-291X
    Keywords: [abr] BSA; bovine serum albumin ; [abr] HEPES; N-2-hydroxyethyl-piperazine-N'-2-ethane sulfonicacid ; [abr] KRH; Kreb's Ringer HEPES buffer
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/S0006-291X(87)80244-9
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  • 6
    Electronic Resource
    Electronic Resource
    Determination of ions, amino acids/amines in the haemolymph of fairy shrimps by capillary electrophoresis with indirect UV and laser-induced fluorescence detection (1996)
    Springer
    Hydrobiologia 319 (1996), S. 103-109 
    Details
    ISSN: 1573-5117
    Keywords: fairy shrimps ; haemolymph ; capillary electrophoresis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We identified and quantified the major inorganic ions present in a haemolymph of four species of fairy shrimp, to determine its ionic strength, using capillary electrophoresis, non-destructive micro-technique for the analysis of small amounts of body fluids. This is the first speciation and quantitative analysis of inorganic ions in the haemolymph of fairy shrimps.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00016878
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  • 7
    Electronic Resource
    Electronic Resource
    Regional and age variations in growing tendon (1994)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 221 (1994), S. 309-320 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Gastrocnemius tendons of 10 White Leghorn chickens at 6, 8, and 12 weeks of age were divided into proximal, middle, and distal portions to assess regional variability in composition and growth. Body weight increases ∼ 150% during the period examined, whereas the lateral gastrocnemius muscle and tendon increase ∼ 193% and 227%, respectively. No significant changes in cellularity (DNA concentration) or hydroxypyridinium (OHP) crosslinks occur with increasing age. Hydroxyproline (HYP) concentration increases by 12 weeks of age, as hexuronate, glucosamine, and galactosamine decrease. Composition shows some regional variation: the distal region of the tendon has a lower HYP concentration, and increased GAGs and OHP crosslinks compared to either the proximal or middle regions, which do not differ from each other. The mean collagen fibril diameter increases with age, but the oldest tendons also contain more small diameter fibrils (〈40 nm). There is a unimodal fibril distribution at all three ages, although this has broadened by 12 weeks. The data from this study suggest that rapid tendon growth occurs throughout the time period examined and that changes characteristic of mature tendon, such as increased OHP crosslink concentration, have not yet developed in hatchlings because of the large amount of new tissue being produced. Whereas all three regions of the tendon are similar in size, composition of the distal region differs from that of the proximal and middle regions, suggesting that this portion of the tendon should be avoided when sampling a tendon. © 1994 Wiley-Liss, Inc.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1052210306
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  • 8
    Electronic Resource
    Electronic Resource
    Cytophysiological basis of disruptive pigmentary patterns in the leopard frog Rana pipiens. I chromatophore densities and cytophysiologyContribution Number 50 from the Amphibian Facility of the University of Michigan. (1973)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 140 (1973), S. 271-284 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Statistical analyses of chromatophore densities and morphological states in wild type, burnsi, and kandiyohi: adult Rana pipiens integument yielded the conclusion that disruptive pigmentary pattern is permanently defined by regional chromatophore densities. Spatial chromatophore patterning is enhanced and rendered more visible by the morphological and physiological differences among chromatophores within the various pattern regions. Specifically, (1) chromatophores were differentialy distributed among pattern regions, (2) greater complexity of gross disruptive patterning had greater underlying melanophores densities, (3) there were significant density differences among non-sib individual animals, among fertilization, and among shipment batches, but not among full-sibs, and (4)kandiyohi individuals had more while burnsi individuals had fewer total melanophores than wild type. A stellate morphology was significantly correlated with high melanophore density, although the relationship of melanophore density to melanophore morphology differed among pattern regions and among genotypes. The functional interrelationships of density and morphology, and their role in disruptive patterning and coloration, are discussed.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051400303
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  • 9
    Electronic Resource
    Electronic Resource
    Mechanics of the syrinx in Gallus gallus. II. Electromyographic studies of Ad Libitum vocalizations (1977)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 152 (1977), S. 1-19 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Records of electrical activity in the tracheal muscles of domestic chickens were obtained for a variety of ad libitum vocalizations. Primary attention was given to an analysis of events during the most complex call, crowing.Three pairs of muscles, Mm. tracheohyoideus, tracheolateralis, and sternotrachealis, can affect the configuration of a chicken's syrinx. The firing patterns of the three muscle pairs are related to their different abilities to affect the tension of the syringeal membranes. The influence of M. tracheohyoideus is most indirect and imprecise, and its role the least clearly defined. It appears to adjust the position of the trachea so that the syrinx is isolated from unpredictable and/or undesirable consequences of nuchal position and tracheal elasticity, and also helps draw the glottis caudad, thereby deepening the pharyngeal chamber. The other two muscles interact to control the tension of the vocal membranes. M. sternotrachealis relaxes the membranes by drawing the drum of the trachea caudad, or, via the syringeal ligament, by rotating the pessulus cranioventrad, or both. M. tracheolateralis tenses the membranes and/or prevents caudal movement of the origin of M. sternotrachealis, a necessity if the syringeal ligament is to rotate the pessulus.Vocalization depends on both syringeal configuration and appropriate air flow. Hence, tracheal muscles, syrinx, air sacs, and ventilatory muscles cooperate to form a vocal system. Cooperation elicits a surprising degree of redundancy. At least one call, a high pitched wail, may be produced by two very different techniques.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051520102
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  • 10
    Electronic Resource
    Electronic Resource
    Ultrastructure of calcified cartilage in the endoskeletal tesserae of sharks (1979)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 160 (1979), S. 75-101 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The tesserate pattern of endoskeletal calcification has been investigated in jaws, gill arches, vertebral arches and fins of the sharks Carcharhinus menisorrah, Triaenodon obesus and Negaprion brevirostris by techniques of light and electron microscopy. Individual tesserae develop peripherally at the boundary between cartilage and perichondrium. An inner zone, the body, is composed of calcified cartilage containing viable chondrocytes separated by basophilic contour lines which have been called Liesegang waves or rings. The outer zone of tesserae, the cap, is composed of calcified tissue which appears to be produced by perichondrial fibroblasts more directly, i.e., without first differentiating as chondroblasts. Furthermore, the cap zone is penetrated by acidophilic Sharpey fibers of collagen. It is suggested that scleroblasts of the cap zone could be classified as osteoblasts. If so, the cap could be considered a thin veneer of bone atop the calcified cartilage of the body of a tessera. By scanning electron microscopy it was observed that outer and inner surfaces of tesserae differ in appearance. Calcospherites and hydroxyapatite crystals similar to those commonly seen on the surface of bone are present on the outer surface of the tessera adjacent to the perichondrium. On the inner surface adjoining hyaline cartilage, however, calcospherites of variable size are the predominant surface feature. Transmission electron microscopy shows calcification in close association with coarse collagen fibrils on the outer side of a tessera, but such fibrils are absent from the cartilaginous matrix along the under side of tesserae. Calcified cartilage as a tissue type in the endoskeleton of sharks is a primitive vertebrate characteristic. Calcification in the tesserate pattern occurring in modern Chondrichthyes may be derived from an ancestral pattern of a continuous bed of calcified cartilage underlying a layer of perichondral bone, as theorized by Ørvig (1951); or the tesserate pattern in these fish may itself be primitive.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051600106
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