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  • Chemistry  (10)
  • Cell & Developmental Biology  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Use of anti-idiotypic antibodies as probes for the interaction of TSH subunits with its receptor (1987)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 34 (1987), S. 151-162 
    Details
    ISSN: 0730-2312
    Keywords: anti-idiotypic antibodies ; thyrotropin subunits ; thyrotropin receptor ; monoclonal antibody ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: TSH is a heterodimeric glycoprotein hormone, whose dissociated subunits are without biological activity. This has precluded the assessment of the relative contribution of each subunit to hormone action. We have raised anti-idiotypes to monoclonal antibodies specific, respectively, for the α and β hTSH subunits. The anti-β anti-idiotype inhibited l25I-hTSH binding to the β subunit-specific monoclonal quantitatively, whereas 125I-hTSH binding to the α subunit-specific monoclonal was not inhibited by anti-α anti-idiotypes, suggesting that only the former is an “internal image” anti-idiotype. Neither of the two anti-idiotypes nor equimolar mixtures thereof inhibited 125I-bTSH binding to thyroid membranes, even though radiolabelled anti-idiotypes showed saturable binding to thyroid plasma membrane which was inhibited 41-65% by bTSH. Each anti-idiotype alone caused 9% inhibition (compared to 50% by NRIgG) of thyroid plasma membrane adenylate cyclase. Equimolar mixtures (125 μg/ml IgG of each anti-idiotype) induced enzyme activity equivalent to 85% of that of 250 mU/ml of TSH. The TSH-like action of the two anti-idiotypes was also reflected in their capacity to increase (450% by 250 μg/ml IgG compared to normal rabbit IgG) the uptake of 131I into isolated thyrocytes and to promote the organization of such cells into follicular structures. At 250 μg/ml, anti-β anti-idiotype promoted the organization of small follicles and only at a concentration of 500 μg/ml did it enhance 131I uptake. Anti α idiotype was without effect in both assays. Lastly, mixture of anti-idiotypes bound to the ∼ 197,000 Mr band (TSH holoreceptor) on protein blots of thyroid plasma membranes resolved on NaDSO4-polyacrylamide gel electrophoresis under non-reducing conditions. Individual anti-idiotypes were without effect.The TSH α and β subunits apparently deliver two cooperative signals to the receptor and that specificity is associated with the β subunit, while the α subunit is important in enhancing receptor affinity for the heterodimer and in stablizing TSH-receptor complex.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcb.240340302
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  • 2
    Electronic Resource
    Electronic Resource
    Biologic activity of anti-thyrotropin anti-idiotypic antibody (1982)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 19 (1982), S. 305-313 
    Details
    ISSN: 0730-2312
    Keywords: anti-iodiotypic antibody ; thyrotropin ; receptor ; thyroid stimulating antibody ; Graves disease ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: We raised an antihuman thyrotropin anti-idiotypic antibody and showed that it was active at the thyrotropin receptor. Thus this antibody inhibited 125I b-TSH binding to thyroid plasma membranes, stimulated adenylate cyclase activity through a guanyl nucleotide-dependent mechanism, increased radioiodide entry rate into isolated porcine thyroid follicular cells, and induced such cultured cells to organize into follicles. All these parameters are typical of thyrotropin action. This work raises the possibility that thyroid stimulating antibodies that cause the hyperthyroidism of Graves disease may be, at least in some patients, anti-thyrotropin anti-idiotypic antibodies. It also offers a novel method whereby antireceptor antibodies used in the isolation and characterization of the receptor may be raised from ligands.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcb.240190402
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  • 3
    Electronic Resource
    Electronic Resource
    Filler effects on the structure and properties of semipermeable polyethylene membranes (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 41 (1990), S. 2513-2516 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1990.070410950
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  • 4
    Electronic Resource
    Electronic Resource
    A mechanical model for the deformational behavior of the polymeric membranes operating in pressure-driven processes (1992)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 46 (1992), S. 1215-1219 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: A mechanical model consisting of three elements (elastic, viscoelastic, and plastic) is proposed to describe the deformational behavior of a polymeric membrane operating in a pressure-driven process. The changes in the membrane thickness are proposed to be calculated from the experimentally obtained flux data. The advantages of a mechanical model over the conventional method of hysteresis area measurement as the mechanical stability of the polymeric membranes are discussed. © 1992 John Wiley & Sons, Inc.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1992.070460711
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  • 5
    Electronic Resource
    Electronic Resource
    Degradable property of UV-cured Hessian cloth (jute) (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 58 (1995), S. 31-39 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Twelve different formulations were developed with urethane acrylate oligomer in combination with a number of functional monomers in the presence of different additives, fillers, and plasticizers. Hessian cloth (jute product) was coated with these formulated solutions and cured under UV radiation. Enhancement of tensile strength and elongation was determined. Loss of these properties due to simulating weathering was also measured. The treated Hessian cloths were buried in soil, water, and mud for a long period and it was found that the samples put in mud were completely degradable while those treated in water and soil retained their tensile properties. © 1995 John Wiley & Sons, Inc.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1995.070580104
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  • 6
    Electronic Resource
    Electronic Resource
    Sulfonation of polyethylene membranes (1991)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 42 (1991), S. 1285-1287 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The polyethylene membranes are hydrophilized under the action of chlorosulfonic acid. Such a hydrophilized membrane shows much better water permeability in comparison with the unmodified one. The ion-exchange capacity of the sulfonated membrane is taken as a measure for the hydrophilicity. The influences of the concentration of the sulfonating agent, temperature, and reaction time, on the ion-exchange capacity and water permeability, are investigated. It is shown that the increase in the degree of sulfonation does not always lead to an increase in the water permeability of the membrane. A lower concentration of the sulfonating agent and a lower temperature are to be chosen, so that only the surface and the inner walls of the pores are sulfonated. Sulfonation in the bulk leads to the deterioration of mechanical properties of the membrane without having any contribution to the increase in the water permeability of the membrane.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1991.070420511
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  • 7
    Electronic Resource
    Electronic Resource
    Direct high-performance liquid chromatographic separation of penbutolol enantiomers on a cellulose tris-3,5-dimethylphenyl carbamate chiral stationary phase (1989)
    New York, NY [u.a.] : Wiley-Blackwell
    Chirality 1 (1989), S. 301-304 
    Details
    ISSN: 0899-0042
    Keywords: β-adrenergic blocker ; chiral resolution ; Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Penbutolol is a β-adrenoceptor blocking agent, and it contains the clinically relevant (-)-S-enantiomer. It was reported that the (+)-R-enantiomer of penbutolol is pharmacologically 50 times less active than the (-)-S-isomer in β-sympatholysis and without intrinsic sympathomimetic activity and refractory period in the heart muscle. Furthermore, the (+)-R-enantiomer does possess mutagenic activity.A high-performance liquid chromatographic (HPLC) method is described for direct identification, stereochemical separation, and quantitation of (+)-R-enantiomer in the clinically used (-)-S-isomer. The method involves the use of cellulose tris-3,5-dimethylphenyl carbamate chiral stationary phase coated on silica gel (OD-Chiralcel column). The capacity factors (k′) for the first eluted enantiomer and stereochemical separation factor (α) obtained were 1.32 and 1.98, respectively. The maximum stereochemical resolution factor (R) was 5.05. The method could be applied for optical purity determination of (-)-(S)-penbutolol in pharmaceutical formulation to detect for the presence of the undesirable (+)-R-enantiomer.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/chir.530010409
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  • 8
    Electronic Resource
    Electronic Resource
    On the deformational characteristics of porous polymeric tubes (1992)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 44 (1992), S. 1899-1903 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The deformational behavior of the porous polymeric tube in the operational period has been studied. The tubes are prepared from a polymeric composition consisting of low-density polyethylene and vulcanized rubber particles. It is established that during operation the tubes undergo only radial elastic deformation. A threshold pressure is necessary to initiate the radial deformation. At a constant pressure, the radial deformation increases with an increase in the polyethylene content in the tube material. The stress necessary to break the tubes during operation is much lower than the tensile strength.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1992.070441104
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  • 9
    Electronic Resource
    Electronic Resource
    On the mechanism of the formation of porous structure in filled polyethylene film by thermomechanical deformation (1992)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 45 (1992), S. 1035-1040 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The pore-formation mechanism in filled polyethylene film during the process of thermomechanical deformation has been studied. The film material consists of a high density polyethylene and SiO2. The rate of deformation as well as the initial thickness of the polyethylene film play a vital role in the formation of the porous structure. With an increase in the deformation rate, the contribution of crazes in the film decreases. Keeping all the parameters constant, with the increase in the initial film thickness, the porosity of the membranes decreases. The mechanism of pore formation is observed by scanning electron microscopy. It is established that the formation of crazes in the deformation process starts from the zones with lower adhesion of the filler with polymer and in the course of the process propagates throughout the mass. The final pore-size distributions as well as the permeability data provide additional indirect evidence in favor of the observed mechanism.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1992.070450612
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  • 10
    Electronic Resource
    Electronic Resource
    Response of filled polyethylene membranes to the changes in the environmental conditions (1992)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 45 (1992), S. 1485-1490 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The environmentally sensitive properties of polyethylene membranes filled with SiO2 have been studied. The porous characteristics of the membranes is investigated with the aqueous solutions of pH 1-8 and aqueous electrolyte solutions with concentrations of 10-5-10-1 mol/L. The experimental results are explained by the concept of coagulation-swelling behavior of the filler under different environmental conditions. The relaxation of membrane resistance with a change in pH is also studied.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1992.070450817
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