ISSN:
1612-1112
Keywords:
Supercritical fluid chromatography
;
Carotenoids
;
β-Carotene
;
Wavelength shift
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary A preliminary investigation of the behaviour of the electronic absorption spectra of selected carotenoids in supercritical fluid chromatography (SFC) with CO2 on a C18 column has been undertaken. A significant hypsochromic shift in the visible (1Bu+) λmax of β-carotene (β,β-carotene), lycopene (ψ,ψ-carotene), zeaxanthin (β,β-carotene-3,3′-diol), canthaxanthin (β,β-carotene-4,4′-dione) and astaxanthin (3,3′-dihydroxy-β,β-carotene-4,4′-dione) in supercritical CO2 was observed in comparison to that in hexane. In addition, the effects of variations in pressure (1500–6000 psi) and temperature (35–50 °C) on the wavelength shift of all-trans-β-carotene have been assessed. Parallel studies were also performed on 15-cis-β-carotene, and a similar hypsochromic shift in the main, visible, λmax was observed. Thecis-peak (1Ag+) of this carotenoid also exhibited a significant, but smaller, hypsochromic shift in comparison to that measured in hexane. However, in contrast to the behaviour of the visible maxima, subsequent alterations to both the pressure and temperature of supercritical CO2 did not further alter the λmax of thecis-peak of 15-cis-β-carotene.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02268278
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