Publication Date:
1986-05-09
Description:
The human N-myc gene is related to the c-myc proto-oncogene, and has been shown to have transforming potential in vitro. Many studies have reported amplification of N-myc in human neuroblastoma and retinoblastoma cell lines. In primary tumors, amplification of the gene was found to correlate directly with behavior of the tumor. Specific restriction fragments of a partial complementary DNA clone of N-myc from LA-N-5 human neuroblastoma cells were placed into a bacterial expression vector for the purpose of producing antigens representative of the N-myc protein. Rabbits immunized with these antigens produced antisera that recognized a protein of 62-64 kilodaltons in neuroblastoma cells. By several criteria, this protein appears to be part of the same proto-oncogene family as the c-myc protein. Moreover, the antisera to fragments of this protein were capable of histochemically identifying malignant cells in clinical specimens.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Slamon, D J -- Boone, T C -- Seeger, R C -- Keith, D E -- Chazin, V -- Lee, H C -- Souza, L M -- CA 16042/CA/NCI NIH HHS/ -- CA 36827/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1986 May 9;232(4751):768-72.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3008339" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Base Sequence
;
Carcinoma, Small Cell/metabolism
;
Immune Sera/immunology
;
Immunoenzyme Techniques
;
Leukemia, Myeloid, Acute/metabolism
;
Lung Neoplasms/metabolism
;
Neoplasm Proteins/genetics/*isolation & purification/physiology
;
Neuroblastoma/metabolism
;
*Oncogenes
;
Proto-Oncogene Proteins/genetics/*isolation & purification/physiology
;
Proto-Oncogene Proteins c-myc
;
Proto-Oncogenes
;
Rabbits/immunology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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