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  • 1
    ISSN: 1612-1112
    Schlagwort(e): Capillary gel electrophoresis ; Laser-induced fluorescence ; DNA sequencing ; Capillary-scanning
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Summary A capillary-scanning system for increasing the total throughput of DNA sequencing has been developed. The DNA sequencing method using the system features a spatial and temporal separation of laser-induced fluorescence detection from capillary gel electrophoresis. Fluorescently labelled adenine base fragments of pBluescript SK(−) were, produced in enzymatic sequencing reactions, and separated by capillary gel electrophoresis using UV-visible transparent capillaries filled with 8% T, 0% C polyacrylamide gel. The capillary containing all bands of the fragments was then scanned longitudinally with a 488 nm argon ion laser beam in 2.6 min. The adenine base sequence of the DNA was determined out to 400 bases by detecting fluorescence signals generated from the bands during the scan. The present scan speed is essentially limited by a slow strip-chart recorder and could be greatly increased by employing a fast data acquisition system.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 18 (1978), S. 849-854 
    ISSN: 0032-3888
    Schlagwort(e): Chemistry ; Chemical Engineering
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Maschinenbau , Physik
    Notizen: An experimental study was carried out to investigate the effects of titanate coupling agents on the rheological properties of p articulate-filled polyolefin melts. Inorganic fillers used were CaCO3, talc, and fiberglass, and polyolefins used were high-density polyethylene (HOPE) and polypropylene (PP). It was found that the addition of the coupling agent TTS to the PP-CaCO3 and PP-fiberglass systems reduced the melt viscosity considerably, whereas the addition of the coupling agent ETDS-201 to the PP-talc and HDPE-talc systems affected the melt viscosity very little. Also carried out was an injection molding study to investigate the effects of different inorganic fillers and the titanate coupling agents used on the mechanical properties of the injection molded specimens. It was found that addition of titanate coupling agents generally resulted in reduced modulus and tensile strength, and increased elongation and impact strength of the filled systems. The PP-CaCO3-TTS and HDPE-talc-ETDS 201 systems were found to have impact strength improved by approximately 100 percent with the addition of a titanate coupling agent. The PP-CaCO3-TTS samples have ultimate tensile elongation approaching that of virgin PP.
    Zusätzliches Material: 4 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 27 (1987), S. 192-201 
    ISSN: 0032-3888
    Schlagwort(e): Chemistry ; Chemical Engineering
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Maschinenbau , Physik
    Notizen: An attempt is made to gain better physical insight on the draw resonance phenomenon occurring during melt spinning of polypropylene (PP). An experimental study is first carried ouit to determine effects of spinning variables and material parameters on draw resonance, using two PRO-FAX PP resins and one PRO-FAX/LDPE blend. Based on the experimental observations, our view on the cause of draw resonance is presented. With this new concept, we are able to explain various aspects of the draw resonance phenomenon observed experimentally during melt spinning of PRO-FAX PP resins. It is concluded that the extensional viscosity versus strain relationship, as determined by an extensional rheometer, is one of the most valuable rheological measurements in analyzing flow instability occurring during polymer processing operations that utilize extensional flows.
    Zusätzliches Material: 12 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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