ISSN:
1432-2048
Keywords:
Canavalia
;
Seed (urease localization)
;
Urease
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Urease (EC 3.5.1.5) is abundantly present in the seeds of many species of Leguminosae. There is at present conflicting information in the literature about its subcellular location and status as a glycoprotein. We have made a study of the subcellular location of urease in jack-bean cotyledons using an immunocytochemical approach; in addition, we studied the biosynthesis and glycoprotein nature of the enzyme using several biochemical approaches. All the results are in agreement with the interpretation that the seed urease is not a glycoprotein, is synthesized on free polysomes, and is present in the cytosol of the storage parenchyma cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00392279
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