Publication Date:
2024-06-12
Description:
Seawater samples were collected with 10-L Niskin bottles in four stations along a cross-shore transect in front of the Port of Mazatlán (Mexico) during the oceanographic cruises Maz IV (April 2018) and Maz V (April 2019) on board the R/V "El Puma" (UNAM). Samples for molecular analyses (abundance of N-cycling genes) were collected at different depths based on the oxygen and chlorophyll-a profiles: surface (5 m depth); mixed layer, deep chlorophyll maximum (at 10–45 m depth); oxycline, base of the oxycline (at 60–125 m depth); upper OMZ (only for Maz V); OMZ core (at 250 m depth); and bottom (between 23 m depth in S1 and 670 m depth in S4). Water samples were filtered on board through polycarbonate membranes (0.22-μm pore, Merck Millipore). Genomic DNA was extracted with the DNeasy PowerWater Kit (Qiagen). The abundance of N-cycling genes was assessed using the gene copy number of bacterial and archaeal amoA, anammox bacterial 16S rRNA, nirK, nirS, and nrfA, using a StepOnePlus real-time PCR system (Applied Biosystems).
Keywords:
Campaign; CTD, Sea-Bird, SBE 19plus; Date/Time of event; Depth, description; DEPTH, water; Elevation of event; El Nino Southern Oscillation; El Puma; Event label; Gene copies per volume; Gene name; Latitude of event 2; Longitude of event; Maz_IV_S1; Maz_IV_S2; Maz_IV_S3; Maz_IV_S4; Maz_V_S1; Maz_V_S2; Maz_V_S3; Maz_V_S4; Maz IV; Maz V; Mexican Pacific; nitrogen genes; oxygen minimum zone; Port of Mazatlán; prokaryotic community; Real-Time PCR, Applied Biosystems, StepOnePlus; Sample code/label; Station label
Type:
Dataset
Format:
text/tab-separated-values, 1620 data points
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