ISSN:
1432-041X
Keywords:
Calliphorin
;
Larval serum proteins (arylphorin)
;
Cuticle
;
Sclerotization
;
Calliphora vicina
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary The stage-specific appearance of calliphorin in cuticles of Calliphora vicina was analysed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. The fate of the protein, injected into last instar larvae, was pursued by autoradiography of histological sections. Fractionation of sclerotized pupal cuticle in buffer-soluble, urea-soluble and NaOH-soluble fractions shows that calliphorin forms covalent and non-covalent links with other cuticle components. Calliphorin traverses the epidermal cells and enters the cuticle in an undegraded state and appears to be an important constituent of the sclerotizing system.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00376062
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