ISSN:
1615-6102
Keywords:
Cell wall formation
;
Calcofluor White
;
Nicotiana tabacum
;
Protoplasts
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary The successful culture of isolated protoplasts of tobacco in the presence of a vital fluorescence stain, Calcofluor White (CW), is reported. The method can be used in both liquid culture and with cells immobilized in agar. Wall formation seems to start at distinct points and can be observed as early as one hour after protoplast isolation. The material either spreads from these points over the entire surface, or the synthesizing apparatus moves in a manner which allows the formation of a continuous layer. The crystallization of the newly synthesized material seems to be hindered by CW, indicating that a rigid cell wall is not a prerequisite for cell division. On the other hand, no cytokinesis occurred unless the protoplasts were surrounded by material showing CW-fluorescence.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01279812
Permalink
