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  • chitinase  (2)
  • small subunit ribulose 1,5 biphosphate carboxylase  (2)
  • Calcium  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Calcium increases the yield of somatic embryos in carrot embryogenic suspension cultures (1990)
    Springer
    Plant cell reports 9 (1990), S. 221-223 
    Details
    ISSN: 1432-203X
    Keywords: Calcium ; Daucus carota ; Somatic embryogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An upward shift in the concentration of calcium present in the medium during somatic embryogenesis increased the number of embryos produced approximately two-fold. This was observed when embryogenic suspension cells grown in 2,4-D medium with the normal calcium concentration of 10−3 M were transferred to hormone-free medium containing 10−2 M calcium and when embryogenic suspension cells grown in 2,4-D medium containing 10−4 M calcium were transferred to hormone-free medium with 10−3 M calcium. At calcium concentrations between 6·10−3 and 10−2 M globular stage somatic embryos were found in cultures supplemented with 2·10−6 M of 2,4-D indicating that elevated calcium counteracts the inhibitory effect of 2,4-D on somatic embryogenesis. No qualitative changes were found in the pattern of extracellular polypeptides as a result of growth and embryogenesis in media with different calcium concentrations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232184
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  • 2
    Electronic Resource
    Electronic Resource
    Molecular cloning of pea mRNAs encoding a shoot-specific polypeptide and light-induced polypeptides (1983)
    Springer
    Plant molecular biology 2 (1983), S. 295-303 
    Details
    ISSN: 1573-5028
    Keywords: cDNA cloning ; light-harvesting chlorophyll a/b protein ; Pisum ; shoot-specific polypeptide ; small subunit ribulose 1,5 biphosphate carboxylase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The molecular cloning of cDNA corresponds to pea seedling mRNA sequences encoding a shoot-specific polypeptide, the small subunit of the ribulose 1,5 biphosphate carboxylase and a component of the light-harvesting chlorophyll a/b complex is described. cDNA prepared from polysomal poly(A)RNA of light-grown shoots was enriched for shoot-specific and light-induced sequences by heterologous liquid hybridization with mercurated polysomal poly(A)RNA of dark-grown roots, followed by sulfhydryl chromatography. Cloned shoot-specific sequences were identified by 2D electrophoretic analysis of hybrid release translation products. The cloned shoot-specific sequence corresponded to a mRNA of 850 nt present both in light-and dark-grown shoots, and produced anin vitro translation product of Mr27 500 and isoelectric point of 4.7.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01578590
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  • 3
    Electronic Resource
    Electronic Resource
    Signal molecules involved in plant embryogenesis (1994)
    Springer
    Plant molecular biology 26 (1994), S. 1305-1313 
    Details
    ISSN: 1573-5028
    Keywords: zygotic embryogenesis ; somatic embryogenesis ; chitinase ; nod factor ; signal molecules
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In plant embryogenesis, inductive interactions mediated by diffusable signal molecules are most likely of great importance. Evidence has been presented that at late globular stages in plant embryogenesis, perturbation of the polar auxin transport results in abberrant embryo morphology. Rhizobium lipooligosaccharides or Nod factors are a newly discovered class of bacterial molecules that are able to trigger initial steps in root nodule development in legumes. Part of the activity of Nod factors may be directed towards alteration of endogenous plant growth regulator balance. The same bacterial Nod factors promoted the formation of globular embryos in the carrot cell line ts11. Whether there exist plant analogues of the Nod factors and whether these molecules are active as a more universal control system perhaps designed to initiate and or mediate gradients in auxin and cytokinin remains to be determined.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00016476
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  • 4
    Electronic Resource
    Electronic Resource
    A shoot-specific mRNA from pea: nucleotide sequence and regulation as compared to light-induced mRNAs (1985)
    Springer
    Plant molecular biology 4 (1985), S. 95-102 
    Details
    ISSN: 1573-5028
    Keywords: light-harvesting chlorophyll a/b protein ; nucleotide sequence ; Pisum ; shoot-specific mRNA ; small subunit ribulose 1,5 biphosphate carboxylase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The regulation of a mRNA encoding a shoot-specific polypeptide from developing pea seedlings was studied and compared to the regulation of mRNAs encoding two major light-induced nuclear-encoded polypeptides, the small subunit of the ribulose 1,5 biphosphate carboxylase (ssRuBPCase) and a polypeptide of the light-harvesting chlorophyll a/b complex (LHCP). By using cDNA clones as probes in Northern blottings of total cellular RNA it was found that both ssRuBPCase and LHCP mRNA could be induced in shoots by white and red light but to lower levels in roots and cotyledons. In contrast, the mRNA for the shoot-specific polypeptide was only found in shoots, and was present approximately two days after the start of germination. The shoot-specific mRNA sequence was predominantly found in stem tissue, irrespective of illumination, both in the young seedlings and adult plants. Only very low amounts could be detected in plumule and leaf. The shoot-specific sequence could also be detected in RNA isolated from developing shoots of another pea cultivar but not in those of other legumes and of cereals. The primary sequence of the complete coding portion and the deduced amino acid sequence of the mRNA encoding the shoot-specific polypeptide was determined. The observed codon usage is non-random and is consistent with data from other high plant genes. Possible polyadenylation signal sequences (AATAAG and AATAAT) were present at 55 and 124 bases 5′ of the poly(A) tail. The polypeptide encoded by the shoot-specific mRNA consists of 196 amino acids with a calculated molecular weight of 21 898. It contains a four times reiterated highly conserved unit of 26 amino acids. The NH2-terminal end is highly hydrophobic and resembles a signal polypeptide.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02418755
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  • 5
    Electronic Resource
    Electronic Resource
    Transient reduction in secreted 32 kD chitinase prevents somatic embryogenesis in the carrot (Daucus carota L.) variant ts11 (1995)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 16 (1995), S. 332-343 
    Details
    ISSN: 0192-253X
    Keywords: Somatic embryogenesis ; temperature-sensitive mutant ts11 ; chitinase ; carrot (Daucus carota L.) ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: At the nonpermissive temperature, somatic embryos of the temperature-sensitive (ts) carrot (Daucus carota L.) cell variant ts11 only proceed beyond the globular embryo stage in the presence of medium conditioned by wild-type cells. The causative component in the conditioned medium has been identified as an acidic 32 kD endochitinase. An antiserum raised against the 32 kD chitinase detected this protein in culture medium from ts11 embryo cultures grown at the permissive temperature as well as at the nonpermissive temperature. No difference in biochemical characteristics or in effect on ts11 embryo development could be detected between the 32 kD chitinase purified from wild-type cultures and the chitinase from ts11 cultures grown at the permissive or at the nonpermissive temperature. Compared to the amount present in a ts11 embryo culture at the permissive temperature, a reduction in the amount of 32 kD chitinase was observed during the temperature-sensitive period at the nonpermissive temperature. These results imply that the arrested embryo phenotype of ts11 is not the result of a structural difference in its 32 kD chitinase, but is the result of a transient decrease in the amount of 32 kD chitinase present. Morphological observations indicate that the ts11 phenotype is pleiotropic and also affects the cell wall of nonembryogenic cells. © 1995 Wiley-Liss, Inc.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020160406
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