Publication Date:
1993-10-15
Description:
Glutamate receptors mediate excitatory neurotransmission in the brain and are important in the formation of memory and in some neurodegenerative disorders. A complementary DNA clone that encoded a 33-kilodalton protein (GR33) was obtained by screening a library with an antibody generated against glutamate binding proteins. The sequence of GR33 is identical to that of the recently reported presynaptic protein syntaxin. When GR33 was expressed in Xenopus oocytes, it formed glutamate-activated ion channels that are pharmacologically similar to those of N-methyl-D-aspartate receptors but with different electrophysiological properties. Mutation of the leucine 278 residue in the single putative transmembrane segment of GR33 affects the properties of the channel. Thus, in vivo GR33 may be a presynaptic glutamate receptor.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Smirnova, T -- Stinnakre, J -- Mallet, J -- New York, N.Y. -- Science. 1993 Oct 15;262(5132):430-3.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Laboratoire de genetique moleculaire de la neurotransmission et des processus neurodegeneratifs, Centre National de la Recherche Scientifique (CNRS), Gif sur Yvette, France.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8105537" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Antigens, Surface/chemistry
;
Brain/embryology
;
Brain Chemistry
;
Calcium/metabolism
;
Cells, Cultured
;
Cloning, Molecular
;
Glutamates/pharmacology
;
Glutamic Acid
;
Humans
;
Membrane Potentials
;
Mutagenesis, Site-Directed
;
N-Methylaspartate/pharmacology
;
Nerve Tissue Proteins/chemistry
;
Neurons/chemistry
;
Oocytes
;
Rats
;
Rats, Wistar
;
Receptors, Glutamate/chemistry/genetics/*metabolism
;
Receptors, N-Methyl-D-Aspartate/metabolism
;
Receptors, Presynaptic/chemistry/genetics/*metabolism
;
Syntaxin 1
;
Xenopus
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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