ISSN:
0192-253X
Keywords:
Dictyostelium
;
glycosylation
;
cell adhesion
;
Life and Medical Sciences
;
Genetics
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
Notes:
The monoclonal antibodies MUD1 and MUD50 recognize peptide and carbohydrate epitopes, respectively, on the Dictyoste-lium discoideum developmentally regulated cell surface glycoprotein PsA. PsA is a putative pre-spore cell adhesion molecule during the slug stage and is anchored to the cell membrane via a phos-phatidylinositol glycan. Here we report on the presence of a PsA-like homolog in several Dictyo-stelium species. The MUD1 epitope is mostly confined to the D. discoideum complex, and only one non-D. discoideum strain reacted strongly with this antibody. By contrast, the mAb MUD50 recognises a homologous molecule in several Dictyostelium species that do not react with MUD1. With mAb MUD50 immunoaffinity chromatography, it was possible to purify and obtain an amino-terminal sequence for the PsA homolog from the Dictvoste-lium sp. strain ZA3A and from one strain of D. pur-pureum.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/dvg.1020110525
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