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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 79 (1990), S. 390-396 
    ISSN: 1432-2242
    Keywords: Beta vulgaris ; Protoplasts ; Regeneration ; CMS ; mtDNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary For our program on the transfer of cytoplasmic male sterility (CMS) by cybridization inBeta vulgaris L. (sugar beet), we have developed a procedure for the isolation and culture of mesophyll protoplasts of sugar beet followed by shoot regeneration. A prerequisite proved to be the presence in the media of n-propylgallate (nPG), a lipoxygenase inhibitor. Sustained divisions were found in all accessions that were tested. Plating efficiencies and regeneration ability varied greatly from one experiment to the other and appeared to be accession-dependent. Shoots could be easily transferred to soil. A majority of the regenerants (72%) retained the diploid chromosome number. Somaclonar variation in phenotype was low (4.9%). Mitochondrial DNA probes, capable of discriminating different cytoplasms ofBeta spp. showed no rearrangements due to the protoplast and in vitro culture phase, indicating that these probes can be used to identify cybrids after asymmetric fusions. The data presented here open up possibilities for genetic engineering using protoplasts in one of the world's most important arable crops.
    Type of Medium: Electronic Resource
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  • 2
    Publication Date: 2019-06-27
    Description: Results are reported for an observation of nuclear gamma rays in the energy range between 0.033 and 12.25 MeV from Centaurus A using a balloon-borne actively collimated NaI(Tl) crystal scintillation counter. The observing procedure is outlined, no systematic errors are found in the data, and power-law fits to the source's energy spectrum are attempted. A power law of approximately 0.86E to the -1.9 power photon/sq cm/sec per keV is shown to give an acceptable fit to the continuum, and the detection of two gamma-ray lines at 1.6 and 4.5 MeV, respectively, is discussed. It is found that the low-energy gamma-ray luminosity of Cen A is 9.4 by 10 to the 43rd power erg/sec for a distance of 5 Mpc and that Cen A is apparently variable in low-energy gamma radiation. It is suggested that the broad feature detected at 1.6 MeV may be due to three blended lines (possibly excited Ne-20, Mg-24, and Si-28), the 4.5-MeV line is most likely due to deexcitation of excited C-12, and the nuclear excitation results from either cosmic-ray bombardment of Cen A's interstellar medium or nucleosynthesis within the source.
    Keywords: SPACE RADIATION
    Type: Astrophysical Journal; 210; Dec. 15
    Format: text
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