ISSN:
1432-072X
Keywords:
Lysoamidase bacteriolytic complex
;
Staphylococcus aureus protoplasts
;
α-Glycerophosphate oxidation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The action of the lysoamidase bacteriolytic complex on Staphylococcus aureus VKM B-209P cells has been studied to obtain protoplasts. The cells in the midlogarithmic phase were the most sensitive to lysoamidase action. It led to local destruction of cell wall due to hydrolysis of the peptidoglycan. Protoplast formation occurred in two steps in the presence of 1 M sucrose. First, osmotically fragile spheroplasts were formed. Then, the protoplasts were released from the destructed cell wall. The protoplast yield was about 80%. The protoplasts preserved the intact ultrastructure and were able to synthesize peptidoglycan fibrillae. Mainly the spheroplasts that maintained the cell-wall residues reversed into bacterial forms. The protoplasts had respiratory activity similar to cells. Respiration of cells and protoplasts was stimulated by various substrates. High rates of oxygen consumption were observed with α-glycerophosphate and ethanol as substrates.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00245348
Permalink
