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Digitale Medien

Thermodynamics of the Binding of Chymotrypsin with the Black-eyed Pea Trypsin and Chymotrypsin Inhibitor (BTCI) (1999)

de Freitas, Sonia M. ; Ikemoto, Hiroaki ; Ventura, Manuel M.

Springer

The protein journal 18 (1999), S. 307-313

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ISSN: 1573-4943

Schlagwort(e): Bowman-Birk inhibitor ; microcalorimetry ; enthalpy ; fluorescence polarization

Quelle: Springer Online Journal Archives 1860-2000

Thema: Chemie und Pharmazie

Notizen: Abstract The binding of α-chymotrypsin to black-eyed pea trypsin/chymotrypsin inhibitor (BTCI) has been studied using the inhibitory activity against the enzyme and the formation of the complex enzyme/inhibitor followed by measurements of fluorescence polarization. Apparent equilibrium constants were estimated for several temperatures and the values obtained range from 0.32 × 107 to 1.36 × 107 M−1. The following values were found from van't Hoff plots: Δ H vh ° = 10.8 kcal mol-1 (from inhibitory assays) and 11.1 kcal mol−1 (from fluorescence polarization); ΔS° = 67.9 and = 67.8 kcal K−1 mol−1, respectively. Calorimetric binding enthalpy was determined (corrected for the ionization heat of the buffer) and the resulting value was ΔH cal ° = 4.9 kcal mol-1. These results indicate that the binding of chymotrypsin to BTCI is an entropically driven process.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1023/A:1021039429014

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Portable microwave test packages for beam-waveguide antenna performance evaluations (1992)

Stewart, Scott R. ; Franco, Manuel M. ; Otoshi, Tom Y.

In: Other Sources

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Publikationsdatum: 2011-08-24

Beschreibung: Portable microwave test packages used to evaluate a new 34-m-diameter beam-waveguide (BWG) antenna are described. The experimental methodology involved transporting test packages to different focal points of the BWG system and making noise temperature, antenna efficiency, and holography measurements. Comparisons of data measured at the different focal points enabled determinations of performance degradations caused by various mirrors in the BWG system. It is shown that, due to remarkable stabilities and accuracies of radiometric data obtained through the use of the microwave test packages, degradations caused by the BWG system were successfully determined.

Schlagwort(e): ELECTRONICS AND ELECTRICAL ENGINEERING

Materialart: IEEE Transactions on Microwave Theory and Techniques (ISSN 0018-9480); 40; 6, Ju

Format: text

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