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  • 1
    Electronic Resource
    Electronic Resource
    Differential transcription of multiple copies of a silk worm gene encoding tRNA"1^G^l^y
    Amsterdam : Elsevier
    Gene 134 (1993), S. 183-190 
    Details
    ISSN: 0378-1119
    Keywords: Bombyx mori ; RNA polymerase III transcription ; gene family ; negative regulation ; positive regulation ; regulatory elements ; tissue specificity ; transcription factors ; transcription regulation
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0378-1119(93)90092-H
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  • 2
    Electronic Resource
    Electronic Resource
    Determination of trace amounts of 5-methylcytosine in DNA by reverse-phase high-performance liquid chromatography
    Amsterdam : Elsevier
    Analytical Biochemistry 164 (1987), S. 164-169 
    Details
    ISSN: 0003-2697
    Keywords: 5-methylcytosine ; Bombyx mori ; Drosophila melanogaster ; HPLC techniques ; trace analysis
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0003-2697(87)90381-2
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  • 3
    Electronic Resource
    Electronic Resource
    Requirement for calcium ions in mycobacteriophage I3 DNA injection and propagation (1980)
    Springer
    Archives of microbiology 124 (1980), S. 249-254 
    Details
    ISSN: 1432-072X
    Keywords: Calcium ions ; Phage propagation ; Phage DNA injection ; Mycobacteriophage 13
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Ca2+ ions are absolutely necessary for the propagation of mycobacteriophage I3 in synthetic medium. These ions are required for successful infection of the host and during the entire span of the intracellular development of the phage. A direct assay of the phage DNA injection using 32[P] labelled phage, showns that Ca2+ ions are necessary for the injection process. The injection itself is a slow process and takes 15 min to complete at 37°C. The bacteria infected in presence of Ca2+ tend to abort if the ions are subsequently withdrawn from the growth medium. The effect of calcium withdrawal is maximally felt during the early part of the latent period; however, later supplementation of Ca2+ ions salvage phage production and the mature phage progeny appear after a delayed interval, proportional to the time of addition of Ca2+.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00427734
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  • 4
    Electronic Resource
    Electronic Resource
    Localization of calcium in murine epidermis following disruption and repair of the permeability barrier (1992)
    Springer
    Cell & tissue research 270 (1992), S. 503-512 
    Details
    ISSN: 1432-0878
    Keywords: Epidermis ; Permeability ; Calcium ions ; Ionic localization ; Mouse (hr/hr)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Perturbation of the cutaneous permeability barrier results in rapid secretion of epidermal lamellar bodies, and synthesis and secretion of new lamellar bodies leading to barrier repair. Since external Ca2+ significantly impedes the repair response, we applied ion capture cytochemistry to localize Ca2+ in murine epidermis following barrier disruption. In controls, the numbers of Ca2+ precipitates in the basal layer were small, increasing suprabasally and reaching the highest density in the stratum granulosum. Barrier disruption with acetone produced an immediate, marked decrease in Ca2+ in the stratum granulosum, accompanied by secretion of lamellar bodies. Loss of this pattern of Ca2+ distribution was associated with the appearance of large Ca2+ aggregates within the intercellular spaces of the stratum corneum. The Ca2+-containing precipitates progressively reappeared in parallel with barrier recovery over 24 h. Disruption of the barrier with tape stripping also resulted in loss of Ca2+ from the nucleated layers of the epidermis, but small foci persisted where the stratum corneum was not removed; in these sites the Ca2+ distribution did not change and accelerated secretion of lamellar bodies was not observed. Following acetone-induced barrier disruption and immersion in isoosmolar sucrose, the epidermal Ca2+ gradient did not return, and both lamellar body secretion and barrier recovery occurred. However, with immersion in isoosmolar sucrose plus Ca2+, the epidermal Ca2+ reservoir was replenished, and both secretion of lamellar bodies and barrier recovery were impeded. These results demonstrate that barrier disruption results in loss of the epidermal Ca2+ reservoir, which may be the signal that initiates lamellar body secretion leading to barrier repair.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00645052
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  • 5
    Electronic Resource
    Electronic Resource
    Breeding systems and pollination inVigna minima (Leguminosae, Papilionoideae) (1987)
    Springer
    Plant systematics and evolution 156 (1987), S. 117-126 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Leguminosae ; Papilionoideae ; Vigna minima ; Polymorphism ; floral breeding systems ; pollination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Four types of floral breeding systems—(i) chasmogamy, (ii) aerial pseudocleistogamy, (iii) subterranean pseudocleistogamy and (iv) obligate subterranean true cleistogamy—are observed in the populations ofVigna minima inhabiting the Western Ghats (India). Five categories of phenotypes are recognized based on the number and kinds of floral breeding systems found in a given individual. The frequencies of different categories of phenotypes not only show intra- and interpopulation variation, but also fluctuate from generation to generation suggesting differences in the genetic structure of populations. This polymorphism in the breeding system of a single species is unique and may be adaptive. Obligate subterranean true cleistogamy and amphicarpy appear to be adaptations to jungle fires and soil erosion.—The flowers are of the flag-blossom type and insect visitors act as tripping agents. The tripping mechanism together with the polymorphic floral breeding system result in a balanced mixture of selfing and outcrossing. Such a recombination system may enhance the fitness ofV. minima which is essentially a colonizing species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00936066
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  • 6
    Electronic Resource
    Electronic Resource
    Nutritional potential ofVigna minima (Roxb.) Ohwi and Ohashi: (1987)
    Springer
    Plant foods for human nutrition 36 (1987), S. 357-365 
    Details
    ISSN: 1573-9104
    Keywords: quantitative variation ; fractions of seed proteins ; amino acid composition ; Vigna minima ; Vigna umbellata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Quantitative variation in different fractions of seed proteins and their amino acid levels in populations ofVigna minima (Roxb.) Ohwi and Ohashi and inV. umbellata cv. IC 1568 — the rice bean — were investigated. Globulin I fraction, together with globulin II, constitutes 38 to 54 per cent of the total seed protein. The alkali soluble (glutelin) fraction is the second largest fraction. Both these fractions show broad range of variation, suggesting a broad genetic base. The profiles are population specific; the coastal population, which contains higher seed protein also possesses maximum levels of globulin I and glutelin fraction suggesting its potentiality for breeding lines with high protein content, high nutritive value, and salt tolerance. Protein content is positively correlated with globulin I and glutelin fractions, which are in turn positively correlated with each other. The amino acid profiles are specific not only to the fractions but also to the populations. The range of variation in the levels of all amino acids in different fractions is broad suggesting substantial genetic diversity. The average levels of lysine and sulphur amino acids are high in globulin I and glutelin fractions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01892357
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  • 7
    Electronic Resource
    Electronic Resource
    Nutritional potential ofVigna minima (Roxb.) Ohwi and Ohashi: (1987)
    Springer
    Plant foods for human nutrition 36 (1987), S. 345-355 
    Details
    ISSN: 1573-9104
    Keywords: seeds ; protein content ; amino acid composition ; yield components ; populations ; Vigna minima ; Vigna umbellata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The seed protein content and amino acid composition of 14 natural populations and their three-generation progenies (grown in different locations) belonging toVigna minima (Roxb.) Ohwi & Ohashi and ofV. umbellata cv IC 1568 have been investigated. The populations ofV. minima were sampled from different ecozones of Western Ghats of Kerala and Tamil Nadu (India). The range of variation in protein levels is narrow, but the protein content of the coastal population is higher than the rice bean suggesting its breeding potential for high protein and salt tolerant lines of rice bean. Although the seed protein content shows genotype × environment interaction, there is a substantial genetic variability among the populations. The tenuous relationship between protein content and yield conponents suggest the presence of correlation breakers which can be utilized in breeding programmes of rice bean. There is a broad genetic base in the levels of essential amino acids, and the range of variation observed is higher than that recorded for different species ofVigna andPhaseolus. The wild relative is nutritionally as good as or superior to the cultigen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01892356
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  • 8
    Electronic Resource
    Electronic Resource
    Interspecific hybridization between rice bean (Vigna umbellata) and its wild relative (V. minima): Fertility-sterility relationships (1986)
    Springer
    Euphytica 35 (1986), S. 1017-1022 
    Details
    ISSN: 1573-5060
    Keywords: Vigna umbellata ; rice bean ; Vigna minima ; interspecific hybridization ; crossability relationships
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The pre-and post-fertilization barriers in the interspecific crosses between Vigna umbellata and V. minima were investigated. In the reciprocal crosses (V. minima as the ♂ parent) the entry of pollen tubes into the ovary was delayed by about 4 h, and no seed set was observed. However, no pre-fertilization barriers were encountered in crosses involving V. minima as the ⧫ parent and V. umbellata as the ♂ parent (normal cross). The delay/absence of divisions in the endosperm and the failure of embryo to divide were the post-fertilization barriers responsible for somatoplastic sterility in normal crosses which yielded a few hybrid seeds. The hybrid seeds showed poor germinability. The F1 hybrids were intermediate between the parents in most morphological characters, and are completely sterile for pollen. Backcrossing of F1 hybrid with both the parents did not restore fertility in the progenies. V. minima is considered as the tertiary gene pool of the rice bean.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00028613
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