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  • 1
    Electronic Resource
    Electronic Resource
    Amplification of nuclear DNA sequences during induced plant cell dedifferentiation
    Amsterdam : Elsevier
    Cell Differentiation 18 (1986), S. 157-161 
    Details
    ISSN: 0045-6039
    Keywords: DNA amplification ; Vicia faba ; cell differentiation
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0045-6039(86)90081-3
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  • 2
    Electronic Resource
    Electronic Resource
    Effect of light and gibberellic acid on cell division in the first foliage leaf of durum wheat (Triticum durum Desf.) (1988)
    Springer
    Planta 173 (1988), S. 257-262 
    Details
    ISSN: 1432-2048
    Keywords: Blue light ; Cell division (GA3 light) ; Leaf growth ; Gibberellin ; Light (GA3, cell division) ; Phytochrome ; Triticum (GA3 light)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The present paper is part of a research program which aims at a quantitative analysis of the effects of light and gibberellic acid (GA3) on growth of the first foliage leaf in durum wheat (Triticum durum Desf.). Since leaf growth is the combined result of the increase in cell number (cell division) and cell enlargement, the influence of light and GA3 treatment on cell division in the basal meristem of the first leaf in two cultivars, Cappelli and Creso, was investigated. Creso is a short-strawed cultivar carrying the Gai 1 gene which influences both plant height and insensitivity to applied GA3. Cell division, as measured by mitotic index, was similar in darkness, continuous red light and dichromatic irradiation (far-red plus red), while lower mitotic rates were observed under continuous far-red light: this indicates that the response of cell division is modulated by a high-irradiance reaction of phytochrome in both cultivars. The two cultivars showed different responses to blue light. In Cappelli, blue light and dichromatic irradiation (blue plus red) gave lower mitotic indices than the dark control, indicating the action of a specific blue-light-absorbing photoreceptor, whereas in Creso the response kinetics to all light regimes which included blue light were more complex. On the basis also of the results obtained with GA3 application in Cappelli, it appears that (i) the hormonal treatment is able to change the pattern of mitotic index only in the presence of the action of a blue-light receptor and (ii) the different responses of the two cultivars could be the result of different endogenous hormonal levels. The importance of the observations in relation to the data for first-leaf longitudinal growth reported in a previous paper (Baroncelli et al. 1984, Planta 160, 298–304) is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00403018
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  • 3
    Electronic Resource
    Electronic Resource
    Effect of light and gibberellic acid on coleoptile and first-foliage-leaf growth in durum wheat (Triticum durum Desf.) (1984)
    Springer
    Planta 160 (1984), S. 298-304 
    Details
    ISSN: 1432-2048
    Keywords: Blue light ; Coleoptile growth ; Elongation growth (GA, light) ; Gibberellin and light ; Leaf growth ; Light, gibberellin and growth ; Triticum (GA, light)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A comparison has been made of the relative effectiveness of light quality and quantity and gibberellic acid (GA3) treatment on the elongation growth of the coleoptile and the first foliage leaf in durum wheat (Triticum durum Desf. cvs. Cappelli and Creso). The cultivar Creso is a shortstrawed variety carrying the Gai 1 gene on chromosome 4A, which influences both plant height and insensitivity to applied gibberellins. The main conclusions are as follows: 1) coleoptile elongation growth appears to be modulated via the fluencerate-dependent action of a blue-light receptor and via a low energy response of phytochrome; 2) the inhibition of first-foliage-leaf growth depends on the operation of a single blue-light-responsive photoreceptor; 3) high energy blue light produces the same inhibitory effect on the two wheat cultivars, whereas at relatively low fluences of white and blue light, the cultivar Creso is more sensitive; 4) the insensitivity to applied GA3 exerted by the gene Gai 1 in Creso is independent of light; 5) in Cappelli, the action of light on coleoptiles appears to be independent of the applied GA3, whereas the hormone is able to change the pattern of growth inhibition of the first-foliage-leaf.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00393410
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  • 4
    Electronic Resource
    Electronic Resource
    Extra DNA synthesis in the dedifferentiating cells ofVicia faba roots (1985)
    Springer
    Protoplasma 124 (1985), S. 213-218 
    Details
    ISSN: 1615-6102
    Keywords: Cell dedifferentiation ; Extra DNA synthesis ; Root ; Vicia faba
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Cell dedifferentiation was induced inVicia faba root tissues by removing the whole root meristem (decapitation) and the behaviour of the nuclear DNA in the dedifferentiating cells was studied by means of cytophotometric and autoradiographic analyses. Cytophotometric determination after Feulgen-staining showed that: 1. the vast majority of nuclei in differentiated cells were in the DNA postsynthetic phase, but their Feulgen absorption was lower than that of DNA postsynthetic nuclei (G2, 4 C) in the meristem; 2. such a Feulgen absorption was detected in certain nuclei after root decapitation; 3. all the mitoses in the dedifferentiating tissues were diploid, fully matching the Feulgen absorption of mitoses in the meristem. After3H-thymidine (3H-T) feeding of the decapitated roots and autoradiography, the following results were obtained: 1. two populations of labeled nuclei, characterized by two different levels of scattered labeling occurred in dedifferentiating tissues, slightly labeled nuclei being much more numerous than heavily labeled nuclei; 2. the percentage of labeled nuclei was much greater than that of DNA presynthetic nuclei in the root tissues; 3. almost all the mitoses were labeled after a 16-hour3H-T feeding; 4. the percentage of slightly labeled nuclei paralleled that of dedifferentiating cells; 5. the duration of the DNA synthesis phase and that of the gap between completion of DNA synthesis and mitosis differed in heavily and slightly labeled nuclei; 6. all nuclei which entered DNA synthesis also entered mitosis. These results are interpreted to mean that: 1. after decapitation, two different DNA syntheses occur in the dedifferentiating root tissues ofV. faba: DNA reduplication in cells which dedifferentiate starting from a DNA presynthetic nuclear condition (heavily labeled nuclei) and extra DNA synthesis in cells which dedifferentiate starting from a DNA postsynthetic nuclear condition (slightly labeled nuclei); 2. extra DNA synthesis is required in these dedifferentiating cells for entry into mitosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01290772
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  • 5
    Electronic Resource
    Electronic Resource
    Cytological localization of fast renaturing and satellite DNA sequences inVicia faba (1985)
    Springer
    Protoplasma 124 (1985), S. 106-111 
    Details
    ISSN: 1615-6102
    Keywords: Cell differentiation ; Cytological hybridization ; Quinacrine banding ; Repetitive DNA ; Satellite DNA ; Vicia faba
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary DNA sequences reassociating within a Cot value of 1.8×10−1 and those producing a light satellite in a CsCl density gradient were isolated fromVicia faba DNA and hybridizedin situ on squashes of roots of the same species. Silver grains were seen to be scattered over both the interphase nuclei and the metaphase chromosomes after hybridization with fast renaturing DNA sequences, indicating these are fairly regularly interspersed in theV. faba genome. Clustered labeling occurred after hybridization with satellite DNA sequences, indicating these are clustered in the genome. The localization of satellite DNA in chromosomes appeared to correspond closely to the position of the bright bands detectable after staining with quinacrine mustard. After hybridization with both DNA probes, labeling intensity over the nuclei of meristematic cells was higher than that over the nuclei of differentiating and/or differentiated cells. These results are discussed in relation to the structure of the cell nucleus, the mechanism of quinacrine banding and to previous data suggesting underrepresentation of nuclear repeated DNA sequences in differentiatingV. faba root cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01279729
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