Publication Date:
2000-12-09
Description:
Genetic engineering of non-beta cells to release insulin upon feeding could be a therapeutic modality for patients with diabetes. A tumor-derived K-cell line was induced to produce human insulin by providing the cells with the human insulin gene linked to the 5'-regulatory region of the gene encoding glucose-dependent insulinotropic polypeptide (GIP). Mice expressing this transgene produced human insulin specifically in gut K cells. This insulin protected the mice from developing diabetes and maintained glucose tolerance after destruction of the native insulin-producing beta cells.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Cheung, A T -- Dayanandan, B -- Lewis, J T -- Korbutt, G S -- Rajotte, R V -- Bryer-Ash, M -- Boylan, M O -- Wolfe, M M -- Kieffer, T J -- New York, N.Y. -- Science. 2000 Dec 8;290(5498):1959-62.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Medicine, University of Alberta, Edmonton, AB T6G 2S2, Canada.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11110661" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Blood Glucose/metabolism
;
Cell Line
;
Cloning, Molecular
;
Diabetes Mellitus, Experimental/metabolism/*therapy
;
Enteroendocrine Cells/*cytology/*metabolism
;
Gastric Inhibitory Polypeptide/biosynthesis/genetics
;
Gene Expression
;
Genetic Engineering
;
*Genetic Therapy
;
Glucose/administration & dosage/*metabolism
;
Glucose Tolerance Test
;
Humans
;
Insulin/biosynthesis/genetics/*metabolism
;
Mice
;
Mice, Transgenic
;
Proinsulin/genetics
;
Promoter Regions, Genetic
;
Protein Precursors/genetics
;
Stem Cells/cytology/metabolism
;
Streptozocin
;
Transfection
;
Transgenes
;
Tumor Cells, Cultured
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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