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  • Chemistry  (3)
  • Biosurfactant  (2)
  • 1
    Digitale Medien
    Digitale Medien
    Effect of addition ofPseudomonas aeruginosa UG2 inocula or biosurfactants on biodegradation of selected hydrocarbons in soil (1992)
    Springer
    Journal of industrial microbiology and biotechnology 10 (1992), S. 87-93 
    Details
    ISSN: 1476-5535
    Schlagwort(e): Availability ; Biosurfactant ; Biodegradation ; Bioemulsifier ; Hydrocarbon ; Persistence ; Pseudomonas aeruginosa ; Solubility
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Summary A laboratory study was undertaken to assess the effect of adding eitherPseudomonas aeruginosa UG2 cells or the biosurfactants produced by this m microorganism on the biodegradation of a hydrocarbon mixture in soil at 20°C over a 2-month incubation period. The addition of 100 μg of UG2 biosurfactants per g soil significantly enhanced the degradation of tetradecane, hexadecene and pristane but not 2-methylnaphthalene, the most water-soluble of the hydrocarbons. Addition of UG2 cells at densities of 106, 107, and 108 per g soil did not have a significant effect on biodegradation of the hydrocarbon mixture.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01583840
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Evaluation of microbial surfactants for recovery of hydrophobic pollutants from soil (1993)
    Springer
    Journal of industrial microbiology and biotechnology 11 (1993), S. 163-170 
    Details
    ISSN: 1476-5535
    Schlagwort(e): Acinetobacter calcoaceticus ; Bioemulsifier ; Biosurfactant ; Hydrophobic ; Hydrocarbon ; Pseudomonas aeruginosa ; Partitioning ; Recovery ; Remediation ; Soil
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Summary Several microbially produced biosurfactants were evaluated for their ability to remove hydrophobic compounds from soil. The biosurfactants produced byPseudomonas aeruginosa UG2 andAcinetobacter calcoaceticus RAG-1 displayed the best results, with recovery of [14C]hexachlorobiphenyl from soil slurries of 48.0 and 41.9%, respectively.P. aeruginosa UG2 produced higher levels of extracellular biosurfactants than four otherP. aeruginosa strains.P. aeruginosa UG2 culture filtrate containing biosurfactants enhanced the recovery of several other individual hydrocarbons and polychlorinated biphenyl compounds, as well as several hydrocarbons in a mixture, from soil. The results, suggest that biosurfactants produced byP. aeruginosa UG2 have the potential for remediation of hydrophobic pollutants in soil environments.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01583718
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Ferrocene-containing polymers as electron transfer mediators in carbon paste electrodes modified with PQQ-dependent aldose dehydrogenase (1995)
    Weinheim : Wiley-Blackwell
    Electroanalysis 7 (1995), S. 941-946 
    Details
    ISSN: 1040-0397
    Schlagwort(e): Biosensor ; Aldose ; Ferrocene ; Carbon paste electrode ; Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie
    Notizen: The aim of this work was to improve an aldose-detecting biosensor based on quinoprotein aldose dehydrogenase (ALDH). Polymer-bound ferrocene derivatives with four different structures were studied as electron transfer mediators between the coenzyme of ALDH, pyrroloquinoline qninone (PQQ), and the conducting electrode material. The ferrocene polymers were mixed into carbon paste, which was used for the preparation of aldose-detecting electrodes by immobilizing ALDH on the carbon paste surface by adsorption. The current responses of the ferrocene polymer modified electrodes were studied and the effect of the polymer structure on the response was examined. The electrodes modified with all four polymers showed maximum response at the working potential around 300 mV (vs. Ag/AgCl). The operational stability of the ferrocene polymer modified electrodes was better than that of corresponding dimethylferrocene modified electrodes. The ferrocene polymer modified electrodes seemed to be suitable for the measurement of real samples.
    Zusätzliches Material: 5 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/elan.1140071006
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  • 4
    Digitale Medien
    Digitale Medien
    Redox polymers for electrocatalytic oxidation of NADH - A random block methyl-siloxane polymer containing meldola blue (1995)
    Weinheim : Wiley-Blackwell
    Electroanalysis 7 (1995), S. 935-940 
    Details
    ISSN: 1040-0397
    Schlagwort(e): Biosensor ; NADH ; Meldola Blue ; Redox polymer ; Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie
    Notizen: A water-insoluble modified methyl-siloxane polymer which held 9-dimethylaminobenzo[a]phenoxazine-7-ium (Meldola Blue) units, was synthesized, characterized, and used to drop-coat solid graphite electrodes. The electrocatalytic oxidation of the nicotinamide adenine dinucleotide (NADH) in the polymer could be described by the Michaelis-Menten type of kinetics as seen from steady-state measurements in 0.25 M phosphate buffer, pH7, at 0 mV (vs. SCE). The electrocatalytic properties of the redox polymer was evaluated using the film thickness as a major variable. The theoretical model found to describe the NADH oxidation at the redox polymer modified electrode, is presented. A maximum NADH sensitivity of 40 μA cm-2 mM-1 at a coverage of 0.5 μg cm-2 was indicated.
    Zusätzliches Material: 4 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/elan.1140071005
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  • 5
    Digitale Medien
    Digitale Medien
    Alteration of the substrate range of haloalkane dehalogenase by site-directed mutagenesis (1998)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 59 (1998), S. 520-523 
    Details
    ISSN: 0006-3592
    Schlagwort(e): haloalkane dehalogenase ; protein engineering ; bioremediation ; Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: We attempted to expand the range of chlorinated solvents degraded by Xanthobacter autotrophicus GJ10 to include trichloroethylene by the rational modification of the enzyme haloalkane dehalogenase. The amino acids Phe164, Asp170, Phe172 and Trp175 were individually replaced with alanine by site-directed mutagenesis. All substitutions produced enzymes with lower than wild type activity with 1,2-dichloroethane. The Phe164Ala and Asp170Ala mutants were 3 and 2 times more active than was the wild type enzyme in dechlorinating 1,6-dichlorohexane. The Asp170Ala mutant resembled the wild type enzyme in its relative activity against longer chain substrates. No mutant was active with trichloroethylene. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 59: 520-523, 1998.
    Zusätzliches Material: 1 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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