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  • Biosensor  (2)
  • Biosurfactant  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Journal of industrial microbiology and biotechnology 10 (1992), S. 87-93 
    ISSN: 1476-5535
    Keywords: Availability ; Biosurfactant ; Biodegradation ; Bioemulsifier ; Hydrocarbon ; Persistence ; Pseudomonas aeruginosa ; Solubility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A laboratory study was undertaken to assess the effect of adding eitherPseudomonas aeruginosa UG2 cells or the biosurfactants produced by this m microorganism on the biodegradation of a hydrocarbon mixture in soil at 20°C over a 2-month incubation period. The addition of 100 μg of UG2 biosurfactants per g soil significantly enhanced the degradation of tetradecane, hexadecene and pristane but not 2-methylnaphthalene, the most water-soluble of the hydrocarbons. Addition of UG2 cells at densities of 106, 107, and 108 per g soil did not have a significant effect on biodegradation of the hydrocarbon mixture.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Journal of industrial microbiology and biotechnology 11 (1993), S. 163-170 
    ISSN: 1476-5535
    Keywords: Acinetobacter calcoaceticus ; Bioemulsifier ; Biosurfactant ; Hydrophobic ; Hydrocarbon ; Pseudomonas aeruginosa ; Partitioning ; Recovery ; Remediation ; Soil
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Several microbially produced biosurfactants were evaluated for their ability to remove hydrophobic compounds from soil. The biosurfactants produced byPseudomonas aeruginosa UG2 andAcinetobacter calcoaceticus RAG-1 displayed the best results, with recovery of [14C]hexachlorobiphenyl from soil slurries of 48.0 and 41.9%, respectively.P. aeruginosa UG2 produced higher levels of extracellular biosurfactants than four otherP. aeruginosa strains.P. aeruginosa UG2 culture filtrate containing biosurfactants enhanced the recovery of several other individual hydrocarbons and polychlorinated biphenyl compounds, as well as several hydrocarbons in a mixture, from soil. The results, suggest that biosurfactants produced byP. aeruginosa UG2 have the potential for remediation of hydrophobic pollutants in soil environments.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1040-0397
    Keywords: Biosensor ; Aldose ; Ferrocene ; Carbon paste electrode ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The aim of this work was to improve an aldose-detecting biosensor based on quinoprotein aldose dehydrogenase (ALDH). Polymer-bound ferrocene derivatives with four different structures were studied as electron transfer mediators between the coenzyme of ALDH, pyrroloquinoline qninone (PQQ), and the conducting electrode material. The ferrocene polymers were mixed into carbon paste, which was used for the preparation of aldose-detecting electrodes by immobilizing ALDH on the carbon paste surface by adsorption. The current responses of the ferrocene polymer modified electrodes were studied and the effect of the polymer structure on the response was examined. The electrodes modified with all four polymers showed maximum response at the working potential around 300 mV (vs. Ag/AgCl). The operational stability of the ferrocene polymer modified electrodes was better than that of corresponding dimethylferrocene modified electrodes. The ferrocene polymer modified electrodes seemed to be suitable for the measurement of real samples.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1040-0397
    Keywords: Biosensor ; NADH ; Meldola Blue ; Redox polymer ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A water-insoluble modified methyl-siloxane polymer which held 9-dimethylaminobenzo[a]phenoxazine-7-ium (Meldola Blue) units, was synthesized, characterized, and used to drop-coat solid graphite electrodes. The electrocatalytic oxidation of the nicotinamide adenine dinucleotide (NADH) in the polymer could be described by the Michaelis-Menten type of kinetics as seen from steady-state measurements in 0.25 M phosphate buffer, pH7, at 0 mV (vs. SCE). The electrocatalytic properties of the redox polymer was evaluated using the film thickness as a major variable. The theoretical model found to describe the NADH oxidation at the redox polymer modified electrode, is presented. A maximum NADH sensitivity of 40 μA cm-2 mM-1 at a coverage of 0.5 μg cm-2 was indicated.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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