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  • Biomolecule analysis  (1)
  • CZE-MS  (1)
  • Capillary isotachophoresis  (1)
  • Electrospray ionization  (1)
  • Fourier transform ion cyclotron  (1)
  • 1
    Electronic Resource
    Electronic Resource
    High performance fourier transform ion cyclotron resonance mass spectrometric detection for capillary electrophoresis (1996)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 19 (1996), S. 617-621 
    Details
    ISSN: 0935-6304
    Keywords: Capillary electrophoresis ; Mass spectrometry ; Electrospray ionization ; Fourier transform ion cyclotron ; Resonance ; Biomolecule analysis ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: We describe the current state of the on-line combination of capillary electrophoresis (CE) electrospray ionization (ESI) Fourier transform ion cyclotron resonance (FTICR) mass spectrometry (MS), and discuss aspects of the FTICR technique relevant to its use as a detection scheme for on-line separations. Aspects including sensitivity, mass resolution, duty cycle, and tandem mass spectrometric capabilities are discussed in the context of online separations with examples from the authors' laboratory.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240191105
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  • 2
    Electronic Resource
    Electronic Resource
    Capillary zone electrophoresis-mass spectrometry with electrospray ionization of peptides and proteins (1989)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Microcolumn Separations 1 (1989), S. 223-229 
    Details
    ISSN: 1040-7685
    Keywords: CZE-MS ; electrospray ionization ; peptides ; proteins ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Capillary zone electrophoresis (CZE) coupled with an on-line mass spectrometric (MS) detection system by an atmospheric pressure electrospray ionization (ESI) interface has been used successfully for Separations of peptide and protein mixtures. For CZE-MS, ESI produces multiply charged molecular ions and thus allows mas spectrometers with a limited mass-to-charge range to analyze proteins having molecular weights greater than 100,000. Various pH buffer systems have been investigated, including buffer systems in which pH 〉 pI (isoelectric point of analytes) and pH « pI are designed to reduce protein adsorption onto silica capillary surfaces and thus improve separation efficiencies. More than 125,000 theoretical plates have been obtained from a CZE-MS separation of less than 1 pmol per component of leucine enkephalin and horse heart myoglobin. CZE-MS of other large peptides and proteins are demonstrated.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mcs.1220010504
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  • 3
    Electronic Resource
    Electronic Resource
    On-line capillary electrophoresis-electrospray ionization mass spectrometry of nucleotides (1995)
    Weinheim : Wiley-Blackwell
    Electrophoresis 16 (1995), S. 389-395 
    Details
    ISSN: 0173-0835
    Keywords: Capillary zone electrophoresis ; Capillary isotachophoresis ; Mass spectrometry ; Tandem mass spectrometry ; Nucleotide separation ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Capillary zone electrophoresis (CZE) and reversed anionic capillary isotachophoresis (CITP) conditions have been developed for the separation of mixtures comprised of monophosphate nucleosides, pyridine and flavin dinucleotides, and monophosphate dinucleosides. Results for the on-line coupling of CZE and CITP with electrospray ionization mass spectrometry (MS) are presented. CITP-tandem MS is utilized to provide both molecular weight and structural information of monophosphate dinucleosides. The fragmentation pattern of dinucleotides in the low collision energy range is described. The resulting mass spectra are readily interpreted in terms of dinucleotide structures. These results demonstrate the new capability for applications for the study of DNA and RNA.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150160165
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