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  • Bioluminescence  (3)
  • Induction  (2)
  • Swarming  (2)
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  • 1
    Digitale Medien
    Digitale Medien
    Factors affecting the cellular expression of bacterial luciferase (1981)
    Springer
    Archives of microbiology 129 (1981), S. 67-71 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Bioluminescence ; Marine bacteria ; Electron transport ; Low oxygen ; Long-chain aldehyde ; Luciferase expression quotient
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The in vivo expression of cellular bacterial luciferase has been defined as the luciferase expression quotient, measured as the ratio of the bioluminescence intensity in vivo to the in vitro activity of luciferase in crude cell extracts. The expression is greater in the presence of inhibitors of the electron transport system such as cyanide and N-heptyl-4-hydroxyquinoline and also at lower oxygen tensions. The higher expression of the cellular luciferase under these conditions is postulated to be due to an increase in the intracellular levels of reduced coenzymes which enhance both the reduction of flavin and the reduction of fatty acid to aldehyde. Both FMNH2 and aldehyde are substrates in the light emitting reaction.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00417183
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    The mechanism of swarming of Vibrio alginolyticus (1975)
    Springer
    Archives of microbiology 104 (1975), S. 67-71 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Vibrio alginolyticus ; Swarming ; Chemotaxis ; Flagella
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Factors leading to swarming of Vibrio alginolyticus cells on solid media were studied. Polar flagellated rods from liquid medium develop into small colonies on solid medium. Byproducts, accumulating in the colony area, induce at certain critical concentrations, the formation of peritrichous flagella and development of long heavily flagellated filaments which swarm away from the high by-product concentrations. Several types of nonswarming mutants were isolated, among them, mutants which lack the capacity to form swarming-inducing byproducts, but can be induced to swarm by byproducts of other mutants incapable of swarming. Different compounds could replace the natural metabolic byproducts; at very low concentration these compounds induce peritrichous flagella and swarming in some of the nonswarming mutants mentioned above. The natural metabolic byproducts accumulating in yeast-extract-peptone medium are suggested to be volatile acids belonging to the valine and isoleucine pathway. Wild-type V. alginolyticus cells cannot swarm on certain substrates but its mutants, able to swarm on many substrates in minimal media, are easily selected.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00447301
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Induction of luciferase synthesis in Beneckea harveyi by other marine bacteria (1979)
    Springer
    Archives of microbiology 120 (1979), S. 87-91 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Luminous bacteria ; Marine bacteria ; Induction ; Beneckea ; Photobacterium
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract It has been previously demonstrated that luciferase synthesis in the luminous marine bacteria, Beneckea harveyi and Photobacterium fischeri is induced only when sufficient concentrations of metabolic products (autoinducers) of these bacteria accumulate in growth media. Thus, when cells are cultured in liquid medium there is a lag in luciferase synthesis. A quantitative bioassay for B. harveyi autoinducer was developed and it was shown that many marine bacteria produce a substance that mimics its action, but in different amounts, (20–130% of the activity produced by B. harveyi) depending on the species and strain. This is referred to as alloinduction. None of the bacteria tested produced detectable quantities of inducer for P. fischeri luciferase synthesis. These findings may have significance with respect to the ecology of B. harveyi and P. fischeri.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00409093
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Induction of swarming in Vibrio parahaemolyticus (1974)
    Springer
    Archives of microbiology 101 (1974), S. 357-363 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Swarming ; Vibrio parahaemolyticus
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Vibrio parahaemolyticus, the flagellated nonswarming marine bacteria were induced to swarm on solid media under three different conditions: growth at 20–26°C on medium containing 1% NaCl, growth on a medium in a sealed Petridish and growth on H2O2-treated medium. The morphological transformations observed in cells during swarming of V. parahaemolyticus are similar to those found jor the naturally swarming Vibrio alginolyticus. The mechanism of swarming in both species involves massive formation of peritrichous flagella and a negative chemotactive response to metabolic byproducts.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00455952
    Standort Signatur Erwartet Verfügbarkeit
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  • 5
    Digitale Medien
    Digitale Medien
    Control of luciferase synthesis in a newly isolated strain of Photobacterium leiognathi (1977)
    Springer
    Archives of microbiology 115 (1977), S. 347-351 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Bacterial bioluminescence ; Photobacterium leiognathi ; Induction ; Luciferase
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract In previous studies with luminous bacteria of all different species it has been reported that the synthesis of luciferase is autoinducible: during growth at low cell densities synthesis is effectively repressed while after induction, at higher cell densities, the rate of synthesis of enzyme is up to five times the growth rate. In this paper we report on newly isolated strains of Photobacterium leiognathi which show continued luciferase synthesis irrespective of the cell density. The specific synthesis rate may nevertheless differ from the rate of growth and depends on the luciferase content of the inoculated cells. A ratio of 1 was established for cells having a maximum luciferase content varying to a ratio of about 2 for cells that contained only 1% of the maximum.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00446462
    Standort Signatur Erwartet Verfügbarkeit
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  • 6
    Digitale Medien
    Digitale Medien
    Detection of genotoxicity of metallic compounds by the bacterial bioluminescence test (1988)
    New York : Wiley-Blackwell
    Journal of Bioluminescence and Chemiluminescence 2 (1988), S. 95-99 
    Details
    ISSN: 0884-3996
    Schlagwort(e): Bioluminescence ; genotoxicity ; Photobacterium fischeri ; Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Twenty metallic compounds were assayed for their genotoxic mutagenic activity by the bioluminescence test restoration of the luminescence of dark mutant of the luminous bacterium Photobacterium fischeri). The activity of the metals was tested in a liquid medium as well as on a solid medium. K2Cr2O7, MnCl2, BeCl2, KH2AsO4, ZnCl2 and Na2WO4 showed strong activity in liquid medium while AgNO3, Cd(OOCCH3)2, CoCl2, CuCl2, HgCl2, Na2SeO3 and Pb(NO3)2 were more active in the solid medium test. BaCl2, Na2MoO4, NaAsO2, NiSO4, Na2SeO4, RbCl, and SnCl2 were not active in the bioluminescence test. The correlation between the genotoxic activity of the tested metallic compounds in the bioluminescence test and other bacterial tests for genotoxic agents as well as the correlation between these results and the carcinogenicity of these compounds is discussed.
    Zusätzliches Material: 2 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/bio.1170020206
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  • 7
    Digitale Medien
    Digitale Medien
    The transcription of bacterial luminescence is regulated by sigma 32 (1988)
    New York : Wiley-Blackwell
    Journal of Bioluminescence and Chemiluminescence 2 (1988), S. 81-93 
    Details
    ISSN: 0884-3996
    Schlagwort(e): Bioluminescence ; Sigma 32 ; luciferase ; Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Luminescence in the marine bacterium, Vibrio fischeri, is regulated by a small molecule, the autoinducer. The transcription of the V. fischeri lux genes also requires a regulatory protein, (luxR), cAMP and CRP. We show that, apart from these components, the transcription of the PR lux operon is also controlled by the activity of σ32 (htpR protein). In luminescent Escherichia coli (E. coli/pChv1), as well as in different marine luminous bacteria and their naturally occurring dark (K) variants, the luminescence system can be induced by starvation under microaerophilic conditions. Heat shock also induces luminescence in htpR+ but not in htpR- strains of E. coli/pChv1.An htpR- mutant of E. coli containing pChv1 is very dim and its luminescence is not induced by starvation or heat shock. The addition of a plasmid bearing the gene for htpR+ into such cells restores their response to starvation and heat shock. Cells of wild type E. coli/pChv1 that have been starved or heat shocked respond to lower concentrations of V. fischeri inducer than untreated cells. These cultures also produce more extracellular inducer than untreated cells. Starvation, heat shock and the presence of σ32 do not induce luminescence in luxl deleted E. coli/pChv1 cells.SOS-inducing agents advance the onset of luminescence in both htpR+ and htpR- strains but not in luxl deleted E. coli/pChvi cells.DNA sequencing of the luxR-luxl region reveals the presence of a promoter region of the kind typical for σ32 at the beginning of the luxl gene. In addition we find a LexA protein-DNA binding site in the non-consensus sequence for the -35 region of the PR operon. It is proposed that the regulatory protein-inducer complex displaces the LexA protein and allows the transcription of the right operon. SOS-inducing agents result in proteolysis of LexA protein and advance the onset of luminescence. σ32 enhances the transcription from the PR operon and thus initiates a positive control circuit. It seems that σ32 is the major controlling element in determining the onset of luminescence both in vivo and in vitro.
    Zusätzliches Material: 14 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/bio.1170020205
    Standort Signatur Erwartet Verfügbarkeit
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