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Low-Cost Methods for Molecular Characterization of Mutant Plants : Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols (2015)

Jankowicz-Cieslak, Joanna ; Huynh, Owen A ; Beshir, Mayada M ; [weitere]

Cham : Springer

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Schlagwort(e): Life sciences ; Nucleic acids ; Plant breeding ; Biology ; Technique ; Life Sciences ; Plant Breeding/Biotechnology ; Biological Techniques ; Nucleic Acid Chemistry

Beschreibung / Inhaltsverzeichnis: Introduction --- Health and Safety Considerations --- Sample Collection and Storage --- Low-Cost DNA Extraction --- PCR Amplification for Low-Cost Mutation Discovery --- Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples --- Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes form Common Weedy Plants --- Example Data --- Conclusions.

Seiten: Online-Ressource (X, 35 pages) , 9 illustrations, 3 illustrations in color

ISBN: 9783319162591

URL: https://link.springer.com/openurl?genre=book&isbn=978-3-319-16259-1

Sprache: Englisch

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Low-Cost Methods for Molecular Characterization of Mutant Plants (2021)

Till, Bradley J. ; Jankowicz-Cieslak, Joanna ; Huynh, Owen A. ; [weitere]

Springer Nature

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Publikationsdatum: 2023-03-03

Beschreibung: Plant Breeding/Biotechnology; Biological Techniques; Nucleic Acid Chemistry

Schlagwort(e): Plant Breeding/Biotechnology ; Biological Techniques ; Nucleic Acid Chemistry ; bic Book Industry Communication::K Economics, finance, business & management::KN Industry & industrial studies::KND Manufacturing industries ; bic Book Industry Communication::K Economics, finance, business & management::KN Industry & industrial studies::KND Manufacturing industries::KNDP Pharmaceutical industries ; bic Book Industry Communication::T Technology, engineering, agriculture::TC Biochemical engineering

Sprache: Englisch

Format: image/jpeg

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