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  • 1
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    Development of disease and virus recovery in transgenic mice containing HIV proviral DNA (1988)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1988-12-23
    Description: Transgenic mice containing intact copies of the human immunodeficiency virus (HIV) proviral DNA were constructed. Founder animals were not viremic for HIV and remained healthy during a 9-month observation period. After being mated with nontransgenic animals, one founder mouse (No. 13) gave rise to F1 progeny that developed a disease syndrome characterized by marked epidermal hyperplasia, lymphadenopathy, splenomegaly, pulmonary lymphoid infiltrates, growth retardation, and death by day 25 of life. Infectious HIV, indistinguishable from parental virus by immunoblot analysis, was recovered from the spleen, lymph nodes, and skin of five of five affected animals.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Leonard, J M -- Abramczuk, J W -- Pezen, D S -- Rutledge, R -- Belcher, J H -- Hakim, F -- Shearer, G -- Lamperth, L -- Travis, W -- Fredrickson, T -- New York, N.Y. -- Science. 1988 Dec 23;242(4886):1665-70.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3201255" target="_blank"〉PubMed〈/a〉
    Keywords: Acquired Immunodeficiency Syndrome/immunology/*microbiology/pathology ; Animals ; DNA Probes ; *DNA, Viral/analysis ; *Disease Models, Animal ; Epidermis/pathology ; HIV/*genetics/immunology/isolation & purification ; HIV Antibodies/analysis ; Lung/pathology ; Lymph Nodes/microbiology/pathology ; Mice ; Mice, Transgenic ; Nucleic Acid Hybridization ; Skin/microbiology/pathology ; Spleen/microbiology/pathology
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 2
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    Genomic heterogeneity of AIDS retroviral isolates from North America and Zaire (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1985-11-22
    Description: In an analysis of the genomic variation of AIDS retroviral isolates from patients living in New York, Alabama, and Zaire, restriction maps were constructed by using seven enzymes, each known to cleave the proviral DNA more than once, in conjunction with Southern blot analysis. The maps of LAV, HTLV-III, and ARV-2 as deduced from their published nucleotide sequences were included in this analysis. The results demonstrated that (i) several "signature" restriction sites were common to all isolates; (ii) with the exception of LAV and HTLV-III, the North American and European isolates were all different from one another and showed no geographical specificity; (iii) the African isolates as a group were more diverse than those from North America and Europe; and (iv) the genomic variability was concentrated within the env gene.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Benn, S -- Rutledge, R -- Folks, T -- Gold, J -- Baker, L -- McCormick, J -- Feorino, P -- Piot, P -- Quinn, T -- Martin, M -- New York, N.Y. -- Science. 1985 Nov 22;230(4728):949-51.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2997922" target="_blank"〉PubMed〈/a〉
    Keywords: Acquired Immunodeficiency Syndrome/*microbiology ; Base Sequence ; Chromosome Mapping ; Cloning, Molecular ; DNA Restriction Enzymes ; DNA, Viral/genetics ; Deltaretrovirus/*genetics ; Democratic Republic of the Congo ; Genes, Viral ; Humans ; North America ; Viral Proteins/genetics
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 3
    Electronic Resource
    Electronic Resource
    Hygromycin resistance is an effective selectable marker for biolistic transformation of black spruce (Picea mariana) (2000)
    Springer
    Plant cell reports 19 (2000), S. 358-362 
    Details
    ISSN: 1432-203X
    Keywords: Key words Picea mariana ; Conifer transformation ; Biolistics ; Hygromycin selection ; Transgenic trees
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Using particle bombardment of mature somatic embryos followed by the induction of secondary embryogenesis in the presence of hygromycin, we produced over 90 lines of transgenic embryonal masses expressing β-glucuronidase from two genotypes of black spruce. Transformation efficiencies of up to 7% (1 transgenic line per 14 embryos bombarded) were achieved by extending the period of selection from 8 to 12 weeks. Proliferation of transformed embryonal masses in the presence of hygromycin had no effect on either embryogenicity or embryo maturation. Southern blot hybridization and PCR amplification confirmed the presence of the hygromycin phosphotransferase gene in genomic DNA. The expression of the β-glucuronidase gene in the needles of regenerated seedlings support the potential for long-term transgene expression in spruce.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050740
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