ISSN:
0006-3592
Keywords:
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
The copolymer styrene-maleic anhydride (SMA) was activated to various forms to create enzyme coupling groups. Carboxypeptidase A (CPA) was Immobilized on these supports to enhance their thermal and chemical stability. Immobilized enzyme retained 60-70% of the original activity. When kept at 60°C, while free enzyme was deactivated within 30 min, the immobilized enzyme retained 40% of initial activity at the end of 3 h. The half-life of free enzyme was only 21 min, while for immobilized enzyme it was enhanced up to 3 h. Also, the immobilized enzyme could be repeatedly used over 50 times retaining almost 50% of original activity.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bit.260270517
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