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  • Articles  (3)
  • Biochemistry and Biotechnology
  • Process Engineering, Biotechnology, Nutrition Technology  (2)
  • Medicine  (1)
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  • Articles  (3)
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  • 1
    Electronic Resource
    Electronic Resource
    A method of automatic pH control of a bicarbonate-CO2 buffer system for the submerged culture of hamster kidney cells (1964)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 6 (1964), S. 147-158 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The equipment and operational techniques are described which were found suitable to control pH in the range 6.8-7.8 pH, within ±0.03 pH units of the desired value, at cell concentrations up to a maximum of 2.5 × 106/ml. The results of batch growth of a suspension strain of the BHK cell (clone 13) under conditions of controlled pH are given and the significance of these results is discussed.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260060204
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Sterilization of tissue-culture medium by filtration on a large laboratory scale (1966)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 8 (1966), S. 153-165 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The design and operation of a permanently-piped plant for sterilizing 10-200 l. of tissue-culture media is described. The plant is sterilized in situ by steam injection, the filtration rate is 2-3 l./min., and the turn-around time for volumes of 100 l. is about 3 hr.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260080113
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Quantitative cytochemistry of blood neutrophils in myelodysplastic syndromes and chronic granulocytic leukaemia (1983)
    New York, NY [u.a.] : Wiley-Blackwell
    Cell Biochemistry and Function 1 (1983), S. 92-96 
    Details
    ISSN: 0263-6484
    Keywords: Blood cells ; leukaemia ; myelodysplasia ; cytochemistry ; neutrophils ; microdensitometer ; lysosomes ; preleukaemia ; maturation ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Quantitative cytochemistry of components of blood neutrophil azurophilic granules (myeloperoxidase, chloroacetate esterase, β-glucuronidase, and acid phosphatase) and specific granules (lactoferrin) has been performed by scanning and integrating micro-densitometry in 13 patients with a myelodysplastic syndrome and 11 patients with chronic granulocytic leukaemia. Both patient groups showed a reduction of enzyme activity in azurophilic granules, and also of lactoferrin, consistent with abnormal development of neutrophil granules. These cytochemical changes in blood neutrophils are similar to those found in acute myeloid leukaemia, are consistent with a leukaemic maturation defect, and may be of diagnostic value.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cbf.290010209
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    Paper (German National Licenses)
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