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  • Biochemistry and Biotechnology  (43)
  • Polymer and Materials Science  (42)
  • 1
    Electronic Resource
    Electronic Resource
    Nuclear magnetic resonance and optical spectroscopic studies of block copolymers of polypeptides. I. Block copoly[(benzyl-L-glulamate)n: (benzyl-L-aspartate)m] (1971)
    New York : Wiley-Blackwell
    Biopolymers 10 (1971), S. 2555-2568 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: In a study of A-B type block copolymers of γ benzyl-L-gultamate and β-benzyl-L-aspartate use has been made of the observations: (1) that for poly aspartate esters the chemical shifts of the α-CH and NH resonances are sensitive to the helix sense, (2) that in both helical and random coil conformations the same resonances of poly-γ-benzyl-L-glutamate are well separated from those of poly aspartates. Since the sense of poly-β-benzyl-L-aspartate is very sensitive to the inclusion of γ-benzyl-L-glutamate residues, the degree of overlap between the blocks can be studied by monitoring the helix sense of the aspartate. The ability of the NMR method to make separate observation of the two blocks removes the necessity of relying on an overall ORD parameter such as b0. The copolymers studied include those having lefthanded, righthanded, and mixed-sense aspartate, corresponding to differing degrees of overlap.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.360101215
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  • 2
    Electronic Resource
    Electronic Resource
    Conformation and folding in histones H1 and H5 (1983)
    New York : Wiley-Blackwell
    Biopolymers 22 (1983), S. 113-118 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Denatured histones H1 and H5 can be readily refolded on salt addition. Their digestion by trypsin leads to limit peptides of about 80 residues having the same nmr and CD spectra as those of the intact parent histones. Scanning microcalorimetry shows that (1) the folded structures of H1 and H5 are located entirely in their limit peptides; (2) both have values of the specific denaturation enthalpy typical for small globular proteins; and that (3) both exhibit a classic “2-state” transition (ΔHdcal = ΔHdvan't Hoff). The heat-denaturation profiles of H5 measured using intrinsic and extrinsic Cotton effect and side-chain nmr peaks do not coincide at all. Only the intrinsic Cotton effects give a Tm and ΔHdvan't Hoff close to that from microcalorimetry. We conclude that these proteins exhibit large-scale side-chain motions that precede the macroscopic cooperative transition.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.360220118
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  • 3
    Electronic Resource
    Electronic Resource
    A study of methods for measuring the tacticity of poly(vinyl chloride) by proton magnetic resonance spectroscopy (1978)
    New York : Wiley-Blackwell
    Die Makromolekulare Chemie 179 (1978), S. 2895-2912 
    Details
    ISSN: 0025-116X
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: 60 MHz undecoupled α-, 90 MHz undecoupled β-, and 90 MHz decoupled α- and β-proton spectra were measured for seven poly(vinyl chloride) samples, four of these being commercial type polymers, the others were prepared at +75°C, +5°C and -30°C. The results were used to assess the preferred approach to the determination of tacticity. The reproducibility of the values from different solutions of a given polymer is significantly worse than that between a series of runs on a particular solution, showing that sampling errors are larger than those from instrumental sources. Although the 60 MHz spectra were found to be unsatisfactory when interpreted on a first order basis, curve fitting methods, which were similar to those used for the other types of spectra and involved the use of reasonable assumptions for chemical shifts and coupling constants, gave results of comparable precision to those from the two types of decoupled spectra and superior to those from the undecoupled 90 MHz spectra. The preferred approach is to combine the results from α- and β-proton spectra and to examine more than one solution of a given material. The applicability of Bernoullian propagation statistics was assumed in the majority of the curve fitting calculations; in the cases where this was not so the results were used to test this assumption and it has proved to be valid.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/macp.1978.021791209
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  • 4
    Electronic Resource
    Electronic Resource
    Rate of crosslinking of gelatin in aqueous solutionPresented at the 11th Canadian High Polymer Forum, Windsor, Ontario, Canada, September 1962. (1964)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 8 (1964), S. 1903-1918 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The rate of crosslinking of concentrated gelatin solutions in water has been measured by means of a gelation timer. Some effects of temperature, pH, crosslinking agent, and gelatin concentration have been measured. The crosslinking agents have varied from fast-acting chrome alum, glutaraldehyde, and a carbodiimide to slow-acting mucobromic acid and dialdehyde starches. Detailed crosslinking mechanisms are discussed for dimethylol urea, mucobromic acid, and several dialdehyde crosslinking agents. Some effects of urea and other additives on the dimethylol urea crosslinking rate are described.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1964.070080436
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  • 5
    Electronic Resource
    Electronic Resource
    Pilot scale affinity chromatography: Purification of β-galactosidase (1974)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 16 (1974), S. 1103-1112 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: β-Galactosidase has been purified from an ammonium sulfate precipitate of E. coli strain ML308 by biospecific adsorption on a column of agarose gel substituted with p-aminophenyl-β-D-thiogalactopyranoside. The system described using a 1.8 liter column has a useful processing capacity of 3.8 × 106 units of β-galactosidase per 2 hr cycle. This corresponds to about 5 g of pure enzyme. An electromechanical timing device operates a set of six solenoid valves and carries out a preset program consisting of sample application, washing, and elation operations.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260160810
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  • 6
    Electronic Resource
    Electronic Resource
    The properties of magnetic supports in relation to immobilized enzyme reactors (1973)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 15 (1973), S. 603-606 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260150318
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  • 7
    Electronic Resource
    Electronic Resource
    Measurement of aerobic batch culture maximum specific growth rate and respiration coefficient using a dissolved oxygen probe (1974)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 16 (1974), S. 531-538 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260160409
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  • 8
    Electronic Resource
    Electronic Resource
    Pulp and paper mill solid wastes as substrates for single-cell protein production (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 21 (1979), S. 561-573 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: We have investigated the upgrading of some typical pulp and paper mill solid wastes into protein-enriched animal feed using the cellulolytic fungus Chaetomium cellulolyticum. The waste residues used were six different primary clarifier sludges and a sample of tertiary centricleaner rejects. These were obtained from mills whose modes of operation spanned the range typically in present-day usage: groundwood, sulfite, semichemical, Kraft, and thermomechanical pulping, with and without bleaching. Crude protein production from the solid waste residues is compared to that obtainable from fermentation of untreated or caustic-pretreated sawdusts. Some of these waste residues, especially the Kraft pulp mill rejects, appear to be promising sources of substrate for single-cell protein production. In these preliminary findings, up to 28% dry weight crude protein content of the product has been obtained at specific growth rates of up to 0.12hr-1 on direct utilization of the wastes.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260210404
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  • 9
    Electronic Resource
    Electronic Resource
    Oxygen transfer in mechanically agitated aqueous systems containing dispersed hydrocarbon (1977)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 19 (1977), S. 661-682 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The effect of dispersed n -dodecane or n -hexadecane on the air-to-aqueous phase overall volumetric oxygen transfer coefficient in a simulated (cell-free) stirred-tank fermentor is described. The oil volume fraction ranged from zero to 0.10; the ionic strength of the aqueous phases was varied from 0 to 0.45. The air-to-aqueous phase coefficients in both oil-free (KLa) and oil-bearing (KLa*) systems were evaluated from unsteady-state experiments using a membrane-covered probe to follow the aqueous phase dissolved oxygen tension.For all systems studied, KLa*/KLa was found to be independent of P/V and vs for all practical purposes. However, for a particular aqueous phase and at a given P/V and vs, the ratio KLa*KLa generally differed from unity. Depending on the combination of hydrocarbon type and volume fraction and the aqueous-phase ionic strength employed, the dispersed hydrocarbon may, in some cases, reduce the rate of oxygen transfer and in others enhance it relative to that of the corresponding oil-free gas-liquid dispersion. Enhancement of the air-to-aqueous transfer rate by such negative spreading coefficient hydrocarbons has not been reported previously.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260190505
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  • 10
    Electronic Resource
    Electronic Resource
    Improved method for the dynamic measurement of mass transfer coefficient for application to solid-substrate fermentation (1981)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 23 (1981), S. 1611-1622 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A new method has been developed for the measurement of overall volumetric mass transfer coefficient (KLa) in gas-liquid-solid systems. This method is based on the examination of gas phase dynamics in a three-phase contactor and consists of measuring continuously the response of the outlet gas composition to a step input change of CO2 in the inlet gas stream. The advantages and limitations of the new method are presented and its sensitivity is discussed on the basis of model predictions. Preliminary results on the implementation of the CO2 method are also reported. Experimental data obtained in a nonviscous electrolyte solution show that the proposed method compares favorably with the conventional dissolved oxygen technique, provided that a correction is made to take account of the difference in diffusivity of oxygen and carbon dioxide.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260230718
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