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1

Digitale Medien

Elicitor-mediated induction of phenylalanine ammonia lyase and tryptophan decarboxylase: Accumulation of phenols and indole alkaloids in cell suspension cultures of Catharanthus roseus (1989)

Seitz, H. U. ; Eilert, U. ; Luca, V. ; [weitere]

Springer

Plant cell, tissue and organ culture 18 (1989), S. 71-78

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ISSN: 1573-5044

Schlagwort(e): Catharanthus ; elicitors ; PAL ; TDC ; phenols ; alkaloids

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Abstract Cell suspension cultures (cell line No 615) of Catharanthus roseus cv. Little Delicata responded to elicitor treatment by accumulating monoterpenoid indole alkaloids and phenolic compounds. The excretion of phenols into the culture medium resulted from the induction of the branch-point enzyme phenylalanine ammonia lyase. The accumulation of alkaloids, however, occurred several hours earlier than the elicitor-mediated induction of tryptophan decarboxylase through which shikimate pathway intermediates are channelled into tryptamine and related indole alkaloids. The results indicate that both pathways for phenol and indole alkaloid biosynthesis responded to elicitor treatment and that no obvious causal relationship between pathways could be deduced from this study.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00033466

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2

Digitale Medien

Production of indole alkaloids by surface immobilized C. roseus cells (1990)

Archambault, J. ; Volesky, B. ; Kurz, W. G. W.

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 35 (1990), S. 660-667

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ISSN: 0006-3592

Schlagwort(e): Chemistry ; Biochemistry and Biotechnology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: A technique was developed to surface immobilize plant cells and was scaled up in laboratory size bioreactors. This technique was shown not to hinder the biosynthetic potential of Catharanthus roseus immobilized cells and to induce a partial release (300 μg/L) of serpentine into the culture medium contrary to suspension cultured cells. The release pattern seemed to follow the biosynthesis trends of the product. This release mechanism could be stimulated by a factor of 10 within 2 h by increasing the pH of the culture from 5.0 to 5.5.

Zusätzliches Material: 5 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/bit.260350703

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3

Digitale Medien

Surface immobilization of plant cells (1989)

Archambault, J. ; Volesky, B. ; Kurz, W. G. W.

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 33 (1989), S. 293-299

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ISSN: 0006-3592

Schlagwort(e): Chemistry ; Biochemistry and Biotechnology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: A novel technique has been developed to immobilize plant cells. The cells are deposited on a surface of manmade fibrous material that provides for strong binding of the plant tissue biomass growing in the submerged culture. The immobilized plant cells remain fully viable. Relatively uniform biomass loadings of up to 20 mg d.w. plant cells/cm2 support material have been attained. All plant cells from the inoculum suspension became attached within the first 24-48 h depending on the support matrix configuration and hydraulic culture conditions. The advantages and scale-up potential of this technique are discussed and compared to other culturing modes.

Zusätzliches Material: 5 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/bit.260330307

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4

Digitale Medien

Microbial production of amino acids. I. The synthesis of lysine and threonine by ustilago species (1962)

Ericson, L.-E. ; Kurz, W. G.

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 4 (1962), S. 23-36

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ISSN: 0006-3592

Schlagwort(e): Chemistry ; Biochemistry and Biotechnology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: The ability of a number of Ustilago species, especially Ustilago maydis (DC.) Cda., to produce lysine and threonine was investigated. The organisms were grown in shake flasks or in 10-l. fermentors. Lysine and threonine were found to be excreted into the medium both in the free and bound form. The bound amino acids could be released by acid hydrolysis or by enzymes from autolyzed cells. The optimal conditions for the release by autolysis were, in the case of Ustilago maydis (DC.) Cda., pH 4.3 and 45°C. An enzyme that could liberate lysine from the bound form(s) occurring in the broth was extracted from cells of Ustilago maydis (DC.) Cda. It exhibited an apparent pH optimum near 4.0. The effect of pH and temperature during the growth phase on the yield of lysine and threonine was studied in 10-l. fermentations.

Zusätzliches Material: 5 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/bit.260040105

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5

Digitale Medien

Microbial production of amino acids. II. The influence of carbon and nitrogen sources and metal ions on growth of Ustilago maydis (DC.) Cda. and on lysine and threonine production (1962)

Kurz, W. G. ; Ericson, L.-E.

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 4 (1962), S. 37-52

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ISSN: 0006-3592

Schlagwort(e): Chemistry ; Biochemistry and Biotechnology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: The ability of Ustilago maydis (DC.) Cda. to grow and to produce lysine and threonine was investigated in shake flask cultures. Growth and production of lysine and threonine increased markedly when aeration was increased. The optimal ratio of glucose to diammonium phosphate in the medium seemed to be approximately 10:1. Ammonium phosphate, ammonium sulfate, ammonium chloride, ammonium nitrate, sodium nitrate, urea, aspartic acid, glutamic acid, and glycine were readily used as nitrogen sources. Growth and amino acid production was poor on ammonium carbonate, ammonium acetate, ammonium citrate, trimethylamine, and betaine.U. maydis (DC.) Cda. was found to grow on a number of different carbohydrates. Besides D-glucose the organism could utilize D-mannose, D-galactose, D-fructose, L-arabinose, D-xylose, D-ribose, sucrose, maltose, and the polyalcoholes D-sorbitol, D-mannitol, and i-inositol. Pectin, dextrin, and corn starch treated with α-amylase could also be used but not untreated corn starch or lactose, cellobiose, D-sedoheptulose, glycerol, or D-glucosamine. The formation of lysine and threonine was better with disaccharides and hexoses than with pentoses.No specific effects on the formation of lysine and threonine could be observed from changes in the concentration of calcium, sodium, potassium, magnesium, zinc, iron, copper, manganese, cobalt, or molybdenum. The requirement of metal ions for growth is discussed.

Zusätzliches Material: 2 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/bit.260040106

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6

Digitale Medien

Continuous phased culture - A technique for growing, analyzing, and using microbial cellsIssued as N.R.C. No. 10941. (1969)

Dawson, P. S. S. ; Kurz, W. O. W.

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 11 (1969), S. 843-851

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ISSN: 0006-3592

Schlagwort(e): Chemistry ; Biochemistry and Biotechnology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: Continuous phased growth produces a culture in which most of the cells in the population are in the same stage of their development. The cell, thereby amplified by the size of the synchronous population, may be examined in the phased culture at any desired growth rate. Changes taking place in the cell after the cell cycle, i.e., post-cycle changes, may be examined by a modification of the procedure. Further systematic applications of the method permit a rational approach to problems of cell growth and metabolism.The phasing technique recognizes the cells as the fundamental unit for experimental investigation, and offers a great potential in the analysis of the cell throughout its cycle, a relatively unexploited field in cell physiology and fermentation. Experiments with yeasts and bacteria illustrate some of the applications and progress made so far.

Zusätzliches Material: 7 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/bit.260110510

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7

Digitale Medien

Development of bioreactors for the culture of surface immobilized plant cells (1990)

Archambault, J. ; Volesky, B. ; Kurz, W. G. W.

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 35 (1990), S. 702-711

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ISSN: 0006-3592

Schlagwort(e): Chemistry ; Biochemistry and Biotechnology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: The scaleup of the technique of plant cell surface immobilization was performed successfully in specifically designed laboratory size bioreactors. The immobilizing matrix was formed into a vertically wound spiral providing for a high immobilizing area-to-volume ratio (0.8-1.2 cm-1). A modified airlift and a mechanically stirred vessel delivered a best bioreactor performance characterized by low biomass frothing and highly efficient plant cell attachment and retention (≥96%). The growth of Catharanthus roseus cells investigated in these bioreactors was found not to be mass transfer limited. It required mild mixing and aeration levels (kLa ∼ 10-15 h-1). The biomass formation pattern of surface immobilized plant cells generally exhibited a linear growth phase followed by a stationary phase characterized by the presence of residual carbohydrates in the medium, contrary to suspension cultures. This behavior was found to depend on the plant cell type and/or line cultured, as well as on the inoculum age. The space restriction and unidirectional growth of the SIPC biofilm combined with the limited availability of essential intracellular nutrients rapidly accumulated from the medium by the stationary phase inoculated plant cells all likely contributed to the culture behavior.

Zusätzliches Material: 6 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/bit.260350707

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