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  • 1
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 0006-3592
    Keywords: sialic acid ; in vivo clearance ; electrophoresis of carbohydrates ; adhesion molecules ; CTLA4 ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: CTLA4 is a membrane receptor on cytotoxic T cells whose interaction with the B7 counterreceptor on B cells is important in alloantigen responses. Soluble recombinant human and murine CTLA4 were produced using either Chinese hamster ovary or NS-0 cell lines. Expression vectors were constructed containing the gene coding for the extracellular domain of CTLA4 fused to either human lgG1 hinge, CH2, and CH3 domains or murine lgG2a hinge, CH2, and CH3 domain genes. These glycoproteins were produced in hollow-fiber or packed-bed-type bioreactors and purified from conditioned media by protein A affinity chromatography. Batches of purified CTLA4lg were analyzed for size, composition, and isoelectric point (pl) patterns by standard protein methods; oligosaccharide and monosaccharide profiles using several carbohydrate specific techniques; and in vivo clearance profiles using a murine model. Significant differences were observed between lots in their pl, clearance, and crbohydrate profiles. Higher overall pl values correlated with accelerated α-phase clearance and changes in oligosaccharide composition as determined by lectin binding analysis and electrophoresis of fluorophore-conjugated carbohydrates. Preparations exhibiting slower clearance profiles had oligosaccharides with higher quantities of N-acetylneuraminic acid and were predominantly of an N-linked biantennary complex-type. Conversely, batches with accelerated clearance profiles had less detectable N-acetylneuraminic acid. Oligosaccharides from murine CTLA4lg produced in NS-0 cells had terminal N-glycolylneuraminic acid but no detectable N-acetylneuraminic acid and had concomitant accelerated clearance. These data suggest that the presence and quantity of N-acetylneuraminic acid is an important component in predicting CTLA4lg plasma clearance rates and that production lots can be analyzed for oligosaccharide heterogeneity and sialic acid content by electrophoresis of fluorophore-conjugated carbohydrates. © 1995 John Wiley & Sons, Inc.
    Additional Material: 9 Ill.
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  • 3
    ISSN: 1075-2617
    Keywords: modes of action ; anti-bacterial ; cecropins ; morphology ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Natural anti-bacterial peptides cecropin B (CB) and its analogs cecropin B-1 (CB-1), cecropin B-2 (CB-2) and cecropin B-3 (CB-3) were prepared. The different characteristics of these peptides, with amphipathic/hydrophobic α-helices for CB, amphipathic/amphipathic α-helices for CB-1/CB-2, and hydrophobic/hydrophobic α-helices for CB-3, were used to study the morphological changes in the bacterial cell, Klebsiella pneumoniae and the leukemia cancer cell, HL-60, by scanning and transmission electron microscopies. The natural and analog peptides have comparable secondary structures as shown by circular dichroism measurements. This indicates that the potency of the peptides on cell membranes is dependent of the helical characteristics rather than the helical strength. The microscopic results show that the morphological changes of the cells treated with CB are distinguishably different from those treated with CB-1/CB-2, which are designed to have enhanced anti-cancer properties by having an extra amphipathic α-helix. The morphological differences may be due to their different modes of action on the cell membranes resulting in the different potencies with lower lethal concentration and higher concentration of 50% inhibition (IC50) of CB on bacterium and cancer cell, respectively, as compared with CB-1/CB-2 (Chen et al. 1997. Biochim. Biophys. Acta 1336, 171-179). In contrast, CB-3 has little effect on either the bacterium or the cancer cell. These results provide microscopic evidence that different killing pathways are involved with the peptides. © 1998 European Peptide Society and John Wiley & Sons, Ltd.
    Additional Material: 6 Ill.
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  • 4
    Publication Date: 2022-05-25
    Description: Author Posting. © The Authors, 2005. This is the author's version of the work. It is posted here by permission of Science in China Press for personal use, not for redistribution. The definitive version was published in Chinese Science Bulletin 51 (2006): 327-330, doi:10.1007/s11434-006-0327-x.
    Description: Mechanical energy input to the oceans is one of the most important factors controlling the oceanic general circulation. The atmosphere transports mechanical energy to the oceans primarily through wind stress, plus changes of the sea level pressure (the so-called atmospheric loading). The rate of mechanical energy transfer into the ocean due to atmospheric loading is calculated, based on TOPEX/POSEIDON data over ten-year period (1993-2002). The rate of total energy input for the world oceans is estimated at 0.04TW (1TW=1012W), and most of this energy input is concentrated in the Southern Oceans and the Storm Tracks in the Northern Hemisphere. This energy input varied greatly with time, and the amplitude of the interannual variability over the past ten years is about 15%.
    Description: WW and CCQ were supported by the National Nature Science Foundation of China through grant 40476010 and Research Fund for the Doctoral Program of Higher Education through grant 20030423011. RXH was supported by the National Aero-Space Administration through Contract No. 1229833 (NRA-00-OES-05).
    Keywords: Mechanical energy ; Atmospheric loading ; Ocean
    Repository Name: Woods Hole Open Access Server
    Type: Preprint
    Format: 157120 bytes
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  • 5
    Publication Date: 2022-05-25
    Description: Author Posting. © American Geophysical Union, 2007. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research 112 (2007): C05036, doi:10.1029/2006JC004046.
    Description: The mixed layer is an important component of the oceanic circulation system. Recent progress in energetics of the oceanic circulation suggests that the amount of external mechanical energy available for mixing is directly linked to the strength of the meridional overturning circulation. Using an analytical two-dimensional model and a three-dimensional numerical model, it is shown that the meridional distribution of mixed layer depth plays an important role in regulating the meridional overturning circulation and poleward heat flux. In fact, if the mixed layer at low and middle latitudes is deeper because of increase in mechanical energy input to the turbulence in the upper ocean, the meridional overturning circulation and poleward heat flux are enhanced in a steady circulation system, and at the same time, it may take less mechanical energy to support the subsurface diapycnal mixing.
    Description: RXH was supported by the National Oceanic and Atmospheric Administration through CICOR Cooperative Agreement NA17RJ1223, CJH was supported by the National Key Basic Research Program of China through grant 2006CB403605, andWWwas supported by the National Natural Science Foundation of China through grant 40476010. This study is also supported through the Chinese 111 Project under Contract B07036.
    Keywords: Mixed layer ; Meridional overturning circulation ; Poleward heat flux ; Mechanical energy
    Repository Name: Woods Hole Open Access Server
    Type: Article
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  • 6
    Publication Date: 2015-04-23
    Description: Author(s): Jian Wang, Wei Xie, Long Zhang, Dan Xu, Wenhui Liu, Jian Lu, Yinglei Wang, Jie Gu, Yan Chen, Xuechu Shen, and Zhanghai Chen We realize the evaporative cooling of a polariton gas and observe the formation of polariton condensate in a potential trap based on a ZnO microwire at room temperature. Due to the three-dimensional confinement, the system is characterized by a well-defined discrete spectrum of polariton states whic... [Phys. Rev. B 91, 165423] Published Wed Apr 22, 2015
    Keywords: Surface physics, nanoscale physics, low-dimensional systems
    Print ISSN: 1098-0121
    Electronic ISSN: 1095-3795
    Topics: Physics
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  • 7
    Publication Date: 2011-12-30
    Description: Author(s): Jing Wang, Wei Li, Peng Cheng, Canli Song, Tong Zhang, Peng Deng, Xi Chen, Xucun Ma, Ke He, Jin-Feng Jia, Qi-Kun Xue, and Bang-Fen Zhu [Phys. Rev. B 84, 235447] Published Thu Dec 29, 2011
    Keywords: Surface physics, nanoscale physics, low-dimensional systems
    Print ISSN: 1098-0121
    Electronic ISSN: 1095-3795
    Topics: Physics
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  • 8
    Publication Date: 2018-06-23
    Description: Author(s): Yong Zhong, Sha Han, Yang Wang, Zhiling Luo, Ding Zhang, Lili Wang, Wei Li, Ke He, Can-Li Song, Xu-Cun Ma, and Qi-Kun Xue We report the atomic-scale structure of epitaxial films of the parent infinite-layer compound SrCu O 2 prepared on SrTiO 3 by molecular beam epitaxy. In situ scanning tunneling microscopy study reveals a stoichiometric copper oxide ( CuO 2 ) -terminated surface featured by 2 × 2 reconstruction, caused prima... [Phys. Rev. B 97, 245420] Published Fri Jun 22, 2018
    Keywords: Surface physics, nanoscale physics, low-dimensional systems
    Print ISSN: 1098-0121
    Electronic ISSN: 1095-3795
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  • 9
    Publication Date: 2017-10-07
    Description: Author(s): Si-Yu Li, Ke-Qin Liu, Long-Jing Yin, Wen-Xiao Wang, Wei Yan, Xu-Qin Yang, Jun-Kai Yang, Haiwen Liu, Hua Jiang, and Lin He A variety of new and interesting electronic properties have been predicted in graphene monolayer doped to Van Hove singularities (VHSs) of its density of state. However, tuning the Fermi energy to reach a VHS of graphene by either gating or chemical doping is prohibitively difficult, owing to their ... [Phys. Rev. B 96, 155416] Published Fri Oct 06, 2017
    Keywords: Surface physics, nanoscale physics, low-dimensional systems
    Print ISSN: 1098-0121
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  • 10
    Publication Date: 2015-11-12
    Description: Author(s): Long-Jing Yin, Jia-Bin Qiao, Wen-Xiao Wang, Wei-Jie Zuo, Wei Yan, Rui Xu, Rui-Fen Dou, Jia-Cai Nie, and Lin He Currently there is a lively discussion concerning Fermi velocity renormalization in twisted bilayers and several contradicted experimental results are reported. Here we study electronic structures of the twisted bilayers by scanning tunneling microscopy (STM) and spectroscopy (STS). The interlayer c… [Phys. Rev. B 92, 201408(R)] Published Tue Nov 10, 2015
    Keywords: Surface physics, nanoscale physics, low-dimensional systems
    Print ISSN: 1098-0121
    Electronic ISSN: 1095-3795
    Topics: Physics
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