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  • Biochemistry and Biotechnology  (9)
  • General Chemistry  (7)
  • 1
    Electronic Resource
    Electronic Resource
    Periodic operation of immobilized live cell bioreactor for the production of candicidin (1991)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 37 (1991), S. 1010-1020 
    Details
    ISSN: 0006-3592
    Keywords: immobilized cells ; periodic operation ; secondary metabolites ; simulation ; candicidin ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A lumped model for cell growth and secondary metabolite production in an immobilized live cell bioreactor has been developed. This model is applied here to simulate the performance of an immobilized bioreactor under steady-state conditions and under conditions of periodically varying concentration of a growth-limiting substrate. The results of the simulation study were experimentally verified in the case of the production of the antibiotic candicidin by Streptomyces griseus in an immobilized bioreactor with forced periodic operation. The results of the studies suggest that periodically operated immobilized live cell bioreactors can provide a potent alternative for the production of non-growth-associated biochemicals, as compared to free cell fermentations, pulsed fermentations with process cycle regeneration, and nonregenerated bioreactors. This work has demonstrated that by frequent pulsing of the growth limiting nutrient, stable extended production can be obtained at high specific cellular productivities.
    Additional Material: 18 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260371105
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  • 2
    Electronic Resource
    Electronic Resource
    Effect of wall growth on continuous biological oxidation of ferrous iron (1974)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 16 (1974), S. 559-563 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260160414
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  • 3
    Electronic Resource
    Electronic Resource
    Kinetic study of sulfide leaching by galvanic interaction between chalcopyrite, pyrite, and sphalerite in the presence of T. ferrooxidans(30°C) and a thermophilic microorganism (55°C) (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 24 (1982), S. 919-940 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A systematic, kinetic study and comparison of the leaching of mixed metal sulfides by galvanic conversion and in the presence of bacteria has been carried out for the first time using both powder (-100 to -400 mesh) and larger (bulk) specimen systems. The rates of dissolution of copper from chalcopyrite and zinc from sphalerite as single, electrically isolated (separate) systems were compared with electrically contacting (galvanically coupled) systems involving CuFeS2/FeS2 and ZnS/FeS2, with and without bacteria and at temperatures of 30 and 55°C. The dissolution of Cu was observed to increase by a factor of 4.6 when the galvanic leaching of CuFeS2/FeS2 was compared to CuFeS2 leaching at 30°C. When bacteria were present, Cu dissolution increased by an additional factor of 2.1 in the CuFeS2/FeS2 system. At 55°C, the corresponding ratios for Cu were 4.3 and 2.7, respectively. The galvanic leaching of Zn in the ZnS/FeS2 system compared to ZnS leaching increased by a factor of 2 at 30°C; in the presence of bacteria the dissolution of Zn from the ZnS/FeS2 system increased by an additional factor of 1.3 at the same temperature. By comparison, the ratio of Cu dissolution from CuFeS2 in acid-bacterial medium and sterile controls (without bacteria) was 5.5. The corresponding ratio for Zn from ZnS was 2.2 at both 30 and 55°C. The order of reaction was found to be essentially first order for the leaching of powder systems at both 30 and 55°C (with T. Ferrooxidans and thermophilic microorganisms, respectively). The corresponding reaction rate constants were observed to be 12.6 and 22.9 for T. ferrooxidans and the thermophilic microorganisms, respectively. Activation energies for the various systems were also determined.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260240413
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  • 4
    Electronic Resource
    Electronic Resource
    Coal desulfurization by leaching involving acidophilic and thermophilic microorganisms (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 24 (1982), S. 2293-2293 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260241020
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  • 5
    Electronic Resource
    Electronic Resource
    Methanol biosynthesis by covalently immobilized cells of Methylosinus trichosporium: Batch and continuous studies (1991)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 37 (1991), S. 551-556 
    Details
    ISSN: 0006-3592
    Keywords: Methylosinus trichosporium ; methanol biosynthesis ; immobilization ; batch and continuous studies ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The DEAE-cellulose linked cells of Methylosinus trichosporium displaying high specific methane mono-oxygenase activity (66 μmol methane oxidized/h mg cells) were used for methanol biosynthesis from biogas derived methane in a batch and a continuous cell reactor. The optimum cell-to-carrier ratio was determined to be 0.5 g cells/g dry weight cellulose. Batch experiments indicated that 100 mM phosphate ion concentration was necessary to inhibit further oxidation of methanol; excess oxygen supply favored methanol accumulation with an increase in methane conversion efficiency to 27%. A pulse of 40 mM sodium formate at the end of 6 h resulted in restoration of methanol accumulation by regenerating NADH2 required for the sustained activity of methane mono-oxygenase. Maximum methanol level of 50 μmol/mg cells was obtained in the batch reactor. In a standard 50-mL ultrafiltration continuous reactor, the covalently linked cells produced methanol at a continuous rate of 100 μmol/h for the first 10 h, after which the methanol accumulation rate fell low due to the depletion of NADH2. The methanol accumulation could be stimulated by supplying sodium formate (40 mM) in either 20 or 100 mM phosphate buffer. Maximum methanol accumulation rate of 267 μmol/h was obtained when 20 mM formate was supplied in the feed stream containing 100 mM phosphate ions, and this level of biosynthesis was maintained for over 72 h. The stoichiometric balance made at various points of formate addition indicated that the molar amount of methanol generated at steady state is dependent on the equimolar addition of sodium formate to the feed. The half-life t1/2 and thermal denaturation rate constant Kd were computed to be 108 h and 6.42 × 10-3 h-1, respectively.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260370609
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  • 6
    Electronic Resource
    Electronic Resource
    Effect of environmental parameters on the efficiency of biodegradation of basalt rock by fungi (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 21 (1979), S. 875-885 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The biodegradation of an aluminum-bearing (basalt) rock by Penicillium simplicissimum has been investigated. This organism grows on a sugar substrate and releases organic acid compounds. These acids interact with the mineral matter and cause their partial decomposition. The dissolved metals are then complexed by the excess organic acids. The activity of the fungi was found to be optimum at an initial pH 7 and in the presence of 5% (w/v) substrate concentration. In 30 days of leaching almost 20% of the aluminum in the rock was solubilized and the pH was decreased from 7 to less than 3.5 in the inoculated flasks. The controls showed less than 1% of the aluminum solubilized and the final pH dropped to only 6.8. A surface characterization study performed by scanning electron microscopy indicated that the specific mineralogical phases containing aluminum and iron within this host rock were preferentially corroded. The mineral phases containing olivine and plagioclase were found to be least resistant, while phases containing titanium were most resistant to the acids released by the fungi.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260210510
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  • 7
    Electronic Resource
    Electronic Resource
    Coal desulfurization by leaching involving acidophilic and thermophilic microorganisms (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 24 (1982), S. 743-748 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260240317
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  • 8
    Electronic Resource
    Electronic Resource
    The role of iron in metal sulfide leaching by galvanic interaction (1983)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 25 (1983), S. 1175-1179 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260250424
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  • 9
    Electronic Resource
    Electronic Resource
    Computer-Aided Organic Synthesis - SESAM: A Simple Program to Unravel “Hidden” Restructured Starting Materials Skeleta in Complex Targets (1998)
    Weinheim : Wiley-Blackwell
    Liebigs Annalen 1998 (1998), S. 1409-1412 
    Details
    ISSN: 1434-193X
    Keywords: Computer-aided organic synthesis ; Retrosynthetic strategy ; Starting materials strategy ; Retrosynthetic disconnections ; Taxol ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: We describe the SESAM program whose aim is to help the chemist in exploring new synthetic strategies for complex structures by locating simple, non-obvious but effective starting materials. The program works at the skeletal level and attempts to map simply modified starting materials into the skeletal surface of the target. SESAM has been tested on taxol and steroid frameworks.
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  • 10
    Electronic Resource
    Electronic Resource
    Golcondan, nonacyclischer C20H24-Käfig-Kohlenwasserstoff mit D2d-SymmetrieDiese Arbeit wurde von der Indian National Science Academy (Verleihung der Ramanujan Research Professorship an G. M.) und dem CSIR (Forschungsstipendium für S.H.K.R.) gefördert. Wir danken Dr. D. S. K. Reddy and Dr. M. S. Reddy für ihr Interesse. (1993)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 105 (1993), S. 1230-1232 
    Details
    ISSN: 0044-8249
    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ange.19931050825
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