ISSN:
1432-072X
Keywords:
Bacillus thuringiensis
;
Delta-endotoxin
;
Mode of action
;
Binding specificity
;
FITC-labelling
;
Antibodies
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Fluorescein isothiocyanate was used as a label to detect delta-endotoxin ofBacillus thuringiensis subsp.thuringiensis andisraelensis in binding studies with different in vitro cell systems. Protoxin of the subspeciesthuringiensis could be labelled directly whereas the activated toxin had to be traced indirectly with labelled antibodies. Both protoxin and activated toxin bound to primary midgut cell cultures ofPieris brassicae larvae as well as to cells of an established culture ofDrosophila melanogaster. No binding with either toxin form could be observed with hemocytes ofP. brassicae. Biological activity as shown by the trypan blue viability assay was obtained only with the activated toxin against the midgut cells. Toxin of the subspeciesisraelensis reacted very unspecifically. Binding followed by rapid destruction was obtained with all the tested cultures.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00690150
Permalink
