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  • 1
    Electronic Resource
    Electronic Resource
    Binding and activity ofBacillus thuringiensis delta-endotoxin to invertebrate cells (1986)
    Springer
    Archives of microbiology 146 (1986), S. 7-11 
    Details
    ISSN: 1432-072X
    Keywords: Bacillus thuringiensis ; Delta-endotoxin ; Mode of action ; Binding specificity ; FITC-labelling ; Antibodies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Fluorescein isothiocyanate was used as a label to detect delta-endotoxin ofBacillus thuringiensis subsp.thuringiensis andisraelensis in binding studies with different in vitro cell systems. Protoxin of the subspeciesthuringiensis could be labelled directly whereas the activated toxin had to be traced indirectly with labelled antibodies. Both protoxin and activated toxin bound to primary midgut cell cultures ofPieris brassicae larvae as well as to cells of an established culture ofDrosophila melanogaster. No binding with either toxin form could be observed with hemocytes ofP. brassicae. Biological activity as shown by the trypan blue viability assay was obtained only with the activated toxin against the midgut cells. Toxin of the subspeciesisraelensis reacted very unspecifically. Binding followed by rapid destruction was obtained with all the tested cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00690150
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  • 2
    Electronic Resource
    Electronic Resource
    Introduction of plasmid pC194 into Bacillus thuringiensis by protoplast transformation and plasmid transfer (1984)
    Springer
    Archives of microbiology 139 (1984), S. 213-217 
    Details
    ISSN: 1432-072X
    Keywords: Bacillus thuringiensis ; Plasmid pC194 ; Transformation ; Plasmid transfer ; Protoplasts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Staphylococcus aureus plasmid pC194 which codes for resistance to chloramphenicol was introduced into six Bacillus thuringiensis strains representing five varieties by protoplast transformation. Six other varieties could not be transformed. pC194 could be identified in transformed strains as autonomous plasmid. The transformed clones contained in addition a new extrachromosomal element of somewhat lower electrophoretic mobility hybridizing with pC194, and pC194 in multimeric forms. pC194 was also transferred from one B. thuringiensis variety to another and from Bacillus thuringiensis to Bacillus subtilis and vice versa by a conjugation-like process, requiring close cell-to-cell contact.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00402002
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    Paper (German National Licenses)
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