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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 59 (1981), S. 25-31 
    ISSN: 1432-2242
    Keywords: Seed proteins ; Wheat ; Barley ; Rye ; Ae. umbellulata ; Ag. elongatum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The seed proteins of ‘Chinese Spring’ wheat stocks which possess single chromosomes from other plant species related to wheat have been separated by gel electrophoresis in the presence of sodium dodecyl sulphate. Marker protein bands have been detected for both arms of barley chromosome 5, chromosome E (= 1R) and B (= 2R) of rye, chromosomes A,B (= 1Cu) and C (= 5Cu) of Aegilops umbellulata and chromosomes I and III of Agropyron elongatum. These studies, and previous findings, indicate that chromosome 5 of barley, chromosome 1R of rye, chromosome I of Ag. elongatum and possibly chromosome 1Cu of Ae. umbellulata are similar to chromosomes 1A, 1B and 1D in hexaploid wheat in that they carry genes controlling prolamins on their short arms and genes controlling high-molecular-weight (apparent molecular weight greater than 86,000) seed protein species on their long arms. These findings support the idea that all these chromosomes are derived from a common ancestral chromosome and that they have maintained their integrity since their derivation from that ancestral chromosome.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 71 (1985), S. 79-92 
    ISSN: 1432-2242
    Keywords: Wheat ; Disulphide-linked proteins ; LMW glutenin subunits ; Genetic Control ; 2-D electrophoresis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) of unreduced total protein extracts from the endosperm of hexaploid wheat revealed three high molecular weight protein bands (triplet bands) in a zone of heavy background streaking. Electrophoretic examination of 135 hexaploid cultivars showed at least five different patterns of these triplet bands. Nine durum wheat cultivars showed a single band only. Analysis of nullisomic-tetrasomic and ditelocentric lines of ‘Chinese Spring’ wheat revealed that the slowest moving band (Tri-1) of the triplet was controlled by gene(s) on chromosome arm 1DS and the fastest moving band (Tri-3) by 1AS. The band with intermediate mobility (Tri-2) was found to be a hybrid aggregate of the subunits controlled by 1DS and 1AS. Using a non-reducing/reducing form of 2-dimensional (2-D) electrophoresis, these triplet bands were shown to be heterotetramers of four subunits designated D (M.W. 58,000), δ (22,000), A (52,000) and α (23,000) where Tri-1=DδDδ, Tri-2 = DδAα and Tri-3 = AαAα. With very low concentrations of 2-mercaptoethanol (ME), the tetramers dissociated into dimeric subunit pairs (Dδ, Aα), the monomers being observed with higher concentrations of ME. The structure of these subunit pairs resembles that of the subunit pairs in the globulin storage proteins of oats and some legumes. The 2-D method employed in this study was useful also for separating low molecular weight (LMW) subunits of glutenin from the monomeric gliadins which have similar electrophoretic mobility in 1-D separation. It was shown that at least four of these LMW glutenin subunits are controlled by genes on 1DS and 1AS and at least one subunit is controlled by gene(s) on 1BS. This electrophoretic separation method has proven useful in understanding the aggregation behaviour of the seed proteins of wheat.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 74 (1987), S. 459-465 
    ISSN: 1432-2242
    Keywords: Wheat ; Glutenins ; Genetic interaction ; Linkage mapping ; SDS-PAGE
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary One-dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) of reduced total protein extracts from the endosperm of hexaploid wheat revealed a new set of faintly-stained bands, having slower electrophoretic mobility than the high-molecular-weight (HMW) glutenin subunits. These new bands have been termed the E group of glutenin subunits. Analysis of aneuploid stocks of Chinese Spring wheat has shown that three of the E bands, in order of increasing electrophoretic mobility, are controlled by genes on the short arms of chromosomes 1B, 1A and 1D, respectively. The E bands are expressed only in the presence of the long arm of chromosome 1B indicating an interaction between two or more genes involved in their production in wheat endosperm. The gene on the short arm of chromosome 1D controlling an E subunit recombined freely with Tri-D1 and the centromere but not at all with Gli-D1, indicating additional complexity at the Gli-DI locus in wheat.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 75 (1988), S. 642-650 
    ISSN: 1432-2242
    Keywords: Wheat ; HMW glutenin subunits ; Linkage mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A translocation mapping procedure was used to map the Glu-1 genes, controlling high-molecular-weight (HMW) glutenin subunits on the long arms of group 1 wheat chromosomes, with respect to their centromeres. The Glu-A1 genes on chromosome arm 1AL were linked with the centromere but gave very different map distances (in cM) of 10.2 ± 3.5 and 33.9 ± 5.4 with test-cross and F2 progeny, respectively. The centromere-Glu-B1 and centromere-Glu-D1 map distances were estimated to be 28.1 ± 2.8 and 30.9 ± 2.7, respectively, using test-cross seeds. A telocentric mapping procedure gave a reduced map distance of 22 ± 3.5 for the centromere-Glu-D1 interval. Possible reasons for the differences between the mapping results obtained with test-cross and F2 seeds and with the translocation and telocentric procedures are discussed.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 60 (1981), S. 333-337 
    ISSN: 1432-2242
    Keywords: Wheat ; Seed proteins ; Glutenin protein ; subunits
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The inheritance of the high-molecular-weight (HMW) glutenin protein subunits in hexaploid wheat has been investigated by using sodium dodecyl sulphate-polyacrylamide gel electrophoresis to examine the segregation of these subunits in 496 test-cross seeds. The parents of the f1 hybrid were chosen so that the test-cross seeds segregated for all the HMW glutenin bands. Two glutenin subunits from one parent, believed to be controlled by genes on chromosome 1D, segregated as alternatives to two glutenin subunits from the other parent, a result that supports the assumption that these subunits are controlled by allelic genes at each of two loci that are very closely linked. Similar results were obtained for glutenin subunits believed to be controlled by chromosome IB, which suggests that these subunits are controlled also by allelic genes at each of two loci that are very closely linked. A single glutenin subunit band, believed to be controlled by chromosome 1A, segregated as an alternative to a single glutenin band from the other parent, except that one seed did not possess either band. It was concluded that these bands are controlled either by allelic genes or by nonallelic genes that are very closely linked.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 83 (1985), S. 327-330 
    ISSN: 1573-5036
    Keywords: Amphiploid ; Elytrigia ; Genome ; Salinity ; Triticum aestivum ; Wheat ; Wheatgrass
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Triticum aestivum cv. Chinese Spring wheat,Elytrigia elongatum (tall wheatgrass), and theTriticum-Elytrigia amphiploid were grown in complete nutrient culture containing, in addition, 0, 40, 80 and 120 mM NaCl. The 3 genotypes responded quite differently to increasing salinity; the Na concentration of wheat shoots increased in direct proportion to the increase in salinity of the external medium whereas the Elytrigia response was interpreted as showing high affinity for Na at low external Na (40 mM) but comparative exclusion of Na at high salinities (120 mM). In contrast, Na levels of the amphiploid were less than those of either wheat or Elytrigia under both low and high salinities. Thus the amphiploid behaved like wheat at 40 mM NaCl but more like Elytrigia at 120 mM NaCl because Na transport to the amphiploid shoot was restricted over the whole salinity range. The K concentration of the amphiploid shoot at high salinities was significantly greater than the K concentrations of either wheat or Elytrigia.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 75 (1988), S. 628-641 
    ISSN: 1432-2242
    Keywords: Wheat ; Triplet proteins ; Gliadins ; Glutenins ; Linkage mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A translocation mapping procedure was used to map gene-centromere distances for the genes controlling endosperm proteins on the short arm of each of the chromosomes 1A, 1B and 1D in wheat. The genes controlling triplet proteins (tentatively designated Tri-1) were found to be closely linked to the centromere on chromosome arms 1AS and 1DS and loosely linked to the gliadin genes (Gli-1) on the same arms. The Gli-1 genes segregated independently or were very loosely linked to their respective centromeres. The Gli-B1-centromere map distance on 1BS was also estimated using conventional telocentric mapping and the result was similar to that obtained with the translocation mapping. A simple two-step one-dimensional electrophoretic procedure is described which allows the low-molecular-weight (LMW) glutenin subunits to be separated from the gliadin bands, thus facilitating the genetic analysis of these LMW subunits. No recombination was observed between the genes (designated Glu-3) controlling some major LMW glutenin subunits and those controlling gliadins on chromosome arms 1AS and 1DS. However, in a separate experiment, the genes controlling LMW glutenin subunits on 1BS (Glu-B3) showed a low frequency of recombination with the gliadin genes.
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  • 8
    Publication Date: 2011-11-10
    Description: Author(s): Arvind Rajaraman, William Shepherd, Tim M. P. Tait, and Alexander M. Wijangco [Phys. Rev. D 84, 095013] Published Wed Nov 09, 2011
    Keywords: Beyond the standard model
    Print ISSN: 0556-2821
    Electronic ISSN: 1089-4918
    Topics: Physics
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  • 9
    Publication Date: 2012-06-23
    Description: Author(s): Elena Accomando, Diego Becciolini, Stefania De Curtis, Daniele Dominici, Luca Fedeli, and Claire Shepherd-Themistocleous Interference effects are widely neglected in searches for new physics. This is the case in recent publications on searches for W ′ bosons using leptonic final states. We examine the effects of interference on distributions frequently used to determine mass limits for possible W ′ bosons and show that ... [Phys. Rev. D 85, 115017] Published Fri Jun 22, 2012
    Keywords: Beyond the standard model
    Print ISSN: 0556-2821
    Electronic ISSN: 1089-4918
    Topics: Physics
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  • 10
    Publication Date: 2011-08-06
    Description: Author(s): Vikram Rentala, William Shepherd, and Shufang Su We discuss same-sign dilepton resonances in the simplified model approach. The relevant SU(3) Q J quantum numbers are 1 2 0,1,2 . For simplicity, we only consider a spin 0 scalar, which is typically referred to as a doubly charged Higgs in the literature. We consider the three simplest cases where the do... [Phys. Rev. D 84, 035004] Published Fri Aug 05, 2011
    Keywords: Beyond the standard model
    Print ISSN: 0556-2821
    Electronic ISSN: 1089-4918
    Topics: Physics
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