Publication Date:
2000-03-04
Description:
The synergistic response of cells to the stimulation of multiple receptors has been ascribed to receptor cross talk; however, the specific molecules that mediate the resultant signal amplification have not been defined. Here a 24-kilodalton single transmembrane protein, designated calcyon, we functionally characterize that interacts with the D1 dopamine receptor. Calcyon localizes to dendritic spines of D1 receptor-expressing pyramidal cells in prefrontal cortex. These studies delineate a mechanism of Gq- and Gs-coupled heterotrimeric GTP-binding protein-coupled receptor cross talk by which D1 receptors can shift effector coupling to stimulate robust intracellular calcium (Ca2+i) release as a result of interaction with calcyon. The role of calcyon in potentiating Ca2+-dependent signaling should provide insight into the D1 receptor-modulated cognitive functions of prefrontal cortex.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Lezcano, N -- Mrzljak, L -- Eubanks, S -- Levenson, R -- Goldman-Rakic, P -- Bergson, C -- MH56608/MH/NIMH NIH HHS/ -- P50 MH068789/MH/NIMH NIH HHS/ -- P50 MH44866/MH/NIMH NIH HHS/ -- R01 MH063271/MH/NIMH NIH HHS/ -- New York, N.Y. -- Science. 2000 Mar 3;287(5458):1660-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Pharmacology and Toxicology, Medical College of Georgia, Augusta, GA 30912-2300, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10698743" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Animals
;
Benzazepines/pharmacology
;
Brain/cytology/metabolism
;
Calcium/metabolism
;
Calcium Signaling
;
Cell Line
;
Cyclic AMP/metabolism
;
Dendrites/chemistry/metabolism
;
Dopamine Agonists/pharmacology
;
Female
;
Heterotrimeric GTP-Binding Proteins/metabolism
;
Humans
;
Macaca mulatta
;
Membrane Proteins/analysis/chemistry/genetics/*metabolism
;
Molecular Sequence Data
;
Prefrontal Cortex/cytology/*metabolism
;
Pyramidal Cells/chemistry/*metabolism
;
Rabbits
;
*Receptor Cross-Talk
;
Receptors, Dopamine D1/analysis/*metabolism
;
Receptors, Neurotransmitter/metabolism
;
Recombinant Fusion Proteins/metabolism
;
Signal Transduction
;
Two-Hybrid System Techniques
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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