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  • 1
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    FEBS Letters 234 (1988), S. 95-99 
    ISSN: 0014-5793
    Keywords: Glycoprotein ; Glycosylation ; Heterologous gene expression ; Invertase ; Transformation ; Yeast
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0983
    Keywords: Keywords Kluyveromyces marxianus ; Yeast ; HIS3 ; Mutants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A method to obtain K. marxianus mutants has been developed. Different auxotrophic mutants were isolated by nystatin and snail-enzyme enrichment procedures using an incubation time of 2 h before adding the antibiotic or the enzyme respectively. All his mutants analyzed by complementation tests turned out to belong to the same complementation group. Some of them were transformed and complemented by the S. cerevisiae HIS3 gene. These non-reverting his3 mutants contain no heterologous sequence, which is essential to make them acceptable for application in the food industry.
    Type of Medium: Electronic Resource
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  • 3
    Publication Date: 2021-05-19
    Description: El objetivo del presente trabajo fue identificar y evaluar los impactos ambientales negativos originados por el hombre, tanto directos como indirectos, en tres de los principales ecosistemas costeros del poblado de Baracoa: la laguna costera El Doctor, la playa Baracoa y el estuario de río del mismo nombre. Para la evaluación de los impactos se empleó la metodología RIAM. Se identificaron un total de 30 impactos, los cuales se vincularon con el factor ambiental que afectan y las acciones que los originan. Se propusieron medidas de mitigación o correctoras para cada caso, con el propósito de combatir o erradicar las causas que los provocan. El análisis de los resultados posibilitó identificar a las aguas superficiales y marinas como el factor ambiental más afectado y la acción más impactante, por frecuencia de aparición, fue el vertimiento de residuales líquidos y sólidos. De los impactos evaluados, seis de ellos se consideran muy altos, 16 altos, cinco moderados, dos bajos y uno muy bajo. Los impactos muy altos y altos, considerados los más importantes, representan alrededor del 73 % del total. Además, 18 impactos son permanentes, 14 irreversibles y solo uno no es acumulativo/sinérgico.
    Description: The objective of the present work was to identify and evaluate the negative direct and indirect environmental impacts caused by man's actions in three of the main coastal ecosystems in Baracoa settlement: El Doctor coastal lagoon, Baracoa Beach and Baracoa river estuary. RIAM methodology was used for impact evaluation. A total of 30 impacts were identified and linked to the environmental factor they affect, as well as to the actions that originate them. In each case, measures were proposed for their mitigation or elimination, with the purpose of counteracting or eradicating their causes. The analysis of these results permitted to identify superficial and marine waters as the most impacted factor; and the most impacting action, due to its frequency of occurrence, turned out to be the discharge of liquid and solid wastes into these waters. The evaluation of the impacts allowed concluding that six of them are considered very high, 16 high, five moderate, two low and one very low. The very high and high impacts, regarded as the most important, represent around 73% of the total. Moreover, 18 impacts are permanent, 14 irreversible, and only one is not cumulative/synergic.
    Description: Published
    Description: Impactos ambientales, medidas de mitigación, playa Baracoa, laguna costera El Doctor, estuario del río Baracoa, Environmental impacts, mitigation measures, Baracoa Beach, El Doctor coastal lagoon, Baracoa river estuary
    Keywords: Water quality ; Beaches ; Water quality ; Beaches ; Man-induced effects
    Repository Name: AquaDocs
    Type: Journal Contribution
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