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  • 1
    Electronic Resource
    Electronic Resource
    Morphology of the dorsal vessel in the abdomen of the blood-feeding insect rhodnius prolixus (1990)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 204 (1990), S. 9-23 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The dorsal vessel (DV) in the abdomen of the blood-feeding insect Rhodnius prolixus was divided functionally into two regions, the heart, into which haemolymph entered the DV through four pairs of ostia located in abdominal segment VII, and the aorta, along which the haemolymph was propelled from abdominal segment VI to the thorax. Osmium-fixed whole mounts revealed the DV to consist of spirally arranged striated muscle fibers and to possess two rows of ventrally attached longitudinal fibers extending the length of the abdomen. Seven pairs of alary muscles were found attached to the DV in the posterior abdominal segments. Contractions of the alary muscles attached to the ventral surface of abdominal segments VII and VIII served to expand the heart. Electron microscopy revealed the DV to consist of a thin layer of contractile elements surrounded by an inner (intima) and outer (adventitia) connective tissue layer. Embedded in the intima along each lateral side of the DV were two large groups of endocardial cells extending the length of the DV. A small group of pericardial cells was embedded in the adventitia along the mid-ventral side of the DV, and clusters of pericardial cells were found attached to the alary muscles. Nerve terminals were found only on the heart: they contained agranular synaptic vesicles approximately 30 nm in diameter and densely stained granules approximately 100-120 nm in diameter. These structural components are discussed in relation to the role of the DV in circulation.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1052040103
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  • 2
    Electronic Resource
    Electronic Resource
    Taxol induces concomitant hyperphosphorylation and reorganization of vimentin intermediate filaments in 9l rat brain tumor cellsIn Memory of Dr. Chi-Shuen Tsou. (1998)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 68 (1998), S. 472-483 
    Details
    ISSN: 0730-2312
    Keywords: taxol ; microtubules ; vimentin ; intermediate filaments ; protein phosphorylation ; protein kinases ; inhibitors ; cytoskeleton ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Taxol, a microtubule stabilizing agent, has been extensively investigated for its antitumor activity. The cytotoxic effect of taxol is generally attributed to its antimicrotubule activity and is believed to be cell cycle dependent. Herein, we report that taxol induces hyperphosphorylation and reorganization of the vimentin intermediate filament in 9L rat brain tumor cells, in concentration- and time-dependent manner. Phosphorylation of vimentin was maximum at 10-6 M of taxol treatment for 8 h and diminished at higher (10-5 M) concentration. Enhanced phosphorylation of vimentin was detectable at 2 h treatment with 10-6 M taxol and was maximum after 12 h of treatment. Taxol-induced phosphorylation of vimentin was largely abolished in cells pretreated with staurosporine and bisindolymaleimide but was unaffected by H-89, KT-5926, SB203580, genistein, and olomoucine. Thus, protein kinase C may be involved in this process. Hyperphosphorylation of vimentin was accompanied by rounding up of cells as revealed by scanning electron microscopy. Moreover, there was a concomitant reorganization of the vimentin intermediate filament in the taxol-treated cells, whereas the microtubules and the actin microfilaments were less affected. Taken together, our data demonstrate that taxol induces hyperphosphorylation of vimentin with concomitant reorganization of the vimentin intermediate filament and that this process may be mediated via a protein kinase C signaling pathway. J. Cell Biochem. 68:472-483, 1998. © 1998 Wiley-Liss, Inc.
    Additional Material: 7 Ill.
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  • 3
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    Mobile Mapping Technologies (2021)
    MDPI - Multidisciplinary Digital Publishing Institute
    Details
    Publication Date: 2024-04-11
    Description: Mobile Mapping technologies have seen a rapid growth of research activity and interest in the last years, due to the increased demand of accurate, dense and geo-referenced 3D data. Their main characteristic is the ability of acquiring 3D information of large areas dynamically. This versatility has expanded their application fields from the civil engineering to a broader range (industry, emergency response, cultural heritage...), which is constantly widening. This increased number of needs, some of them specially challenging, is pushing the Scientific Community, as well as companies, towards the development of innovative solutions, ranging from new hardware / open source software approaches and integration with other devices, up to the adoption of artificial intelligence methods for the automatic extraction of salient features and quality assessment for performance verification The aim of the present book is to cover the most relevant topics and trends in Mobile Mapping Technology, and also to introduce the new tendencies of this new paradigm of geospatial science.
    Keywords: TA1-2040 ; T1-995 ; LRF ; smartphone ; unmanned vehicle ; sensor fusion ; 2D laser scanner ; semantic enrichment ; Vitis vinifera ; indoor scenes ; terrestrial laser scanning ; vine size ; quadric fitting ; multi-group-step L-M optimization ; grammar ; MLS ; indoor topological localization ; trajectory fusion ; second order hidden Markov model ; room type tagging ; fingerprinting ; restoration ; laser scanning ; map management ; Lidar localization system ; point clouds ; binary vocabulary ; self-calibration ; 3D processing ; cultural heritage ; encoder ; category matching ; indoor mapping ; convolutional neural network (CNN) ; tunnel cross section ; visual positioning ; enhanced RANSAC ; segmentation-based feature extraction ; image retrieval ; handheld ; crowdsourcing trajectory ; visual landmark sequence ; indoor localization ; mobile mapping ; rapid relocation ; sensors configurations ; precision agriculture ; 3D digitalization ; mobile laser scanning ; robust statistical analysis ; plant vigor ; motion estimation ; visual simultaneous localization and mapping ; dynamic environment ; Bayesian inference ; automated database construction ; portable mobile mapping system ; SLAM ; small-scale vocabulary ; ORB-SLAM2 ; LiDAR ; IMMS ; point cloud ; optical sensors ; tunnel central axis ; constrained nonlinear least-squares problem ; 3D point clouds ; wearable mobile laser system ; geometric features ; 2D laser range-finder ; RGB-D camera ; OctoMap ; thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TB Technology: general issues::TBX History of engineering and technology
    Language: English
    Format: application/octet-stream
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  • 4
    Electronic Resource
    Electronic Resource
    Arthropod immune system: I. Comparative light and electron microscopic accounts of immunocytes and other hemocytes of Blattella germanica (Dictyoptera: Blattellidae) (1988)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 198 (1988), S. 257-267 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Light microscopy (LM) and scanning (SEM) and transmission electron microscopy (TEM) disclose seven types of hemocytes in adults of the German cockroach, Blattella germanica (Dictyoptera: Blattellidae) - namely, prohemocytes (PR), granulocytes (GR), plasmatocytes (PL), spherulocytes (SP), adipohemocytes (AD), coagulocytes (CO), and oenocytoids (OE). Their shape, size, surface appearance, and internal ultrastructure are characterized here. The shapes and relative sizes of hemocytes noted in vitro have been compared with those flowing through the wing veins (in vivo). The GRs constitute more than 90% of the hemocyte population and have roles in the defense system such as encapsulation and phagocytosis. They are characterized by numerous cytoplasmic granules and marginal microtubular bundles, which are commonly noted in the blood cells of both vertebrates and invertebrates, but have been rarely reported in insect hemocytes. The PRs are characterized by being spherical and small, having a relatively large nucleus and a small area of cytoplasm. The polymorphic PLs have some micropapillae on the cell surface and fewer granules than the GRs. The SPs are characterized by an oblate shape, raspberrylike appearance in SEM, and many spherules in the cytoplasm. The ADs are spherical and have large, refringent fat droplets. The COs possess a distinctive cartwheellike appearance when observed by LM but resemble GRs in TEM preparations. The OEs are opaque and often have an eccentric nucleus. Of the seven types of hemocytes, all, except the COs, can be recognized both in vivo and in vitro by LM; the COs can be recognized only in vitro. SEM does not allow the COs to be distinguished from the GRs, except in samples in which coagulation has occurred. All hemocyte types can be recognized by TEM. Thus, all three types of microscopy allow the various types of hemocytes to be recognized; unequivocal discrimination among the cells is best achieved by combining all the three kinds of microscopy.
    Additional Material: 24 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051980302
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  • 5
    Electronic Resource
    Electronic Resource
    Morphology of neurosecretory cells delineated with cobalt applied extracellularly to the cephalic aorta of the insect Rhodnius prolixus (1988)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 195 (1988), S. 17-29 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Cobalt applied extracellularly to the cephalic aorta in Rhodnius prolixus filled neurosecretory cells (NSCs) located in the brain, the retrocerebral complex, and the suboesophageal ganglion (SOG). Axons of these cells converged over the corpora cardiaca and corpus allatum and merged into a large tract before travelling posteriorly along the ventral side of the aorta. Cobalt-filled cells in the posterior margins of the brain and the retrocerebral complex lacked extensive dendritic arborizations, suggesting that their cell bodies and/or axonal processes in the retrocerebral complex are directly involved with integrative processes determining hormone release. Cobalt-filled cell bodies in the anterior region of the brain were closely associated with the ocellar nerve, and the cobalt-filled cells in the SOG formed extensive dendritic arborizations in the neuropile, suggesting the involvement of sensory cells in regulation of their electrical activity. The ability to fill NSCs with cobalt applied to the aorta demonstrates that the cephalic aorta of R. prolixus is an important neurohaemal region.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051950103
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  • 6
    Electronic Resource
    Electronic Resource
    Filamin translocation is an early endothelial cell inflammatory response to bradykinin: Regulation by calcium, protein kinases, and protein phosphatases (1996)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 62 (1996), S. 383-396 
    Details
    ISSN: 0730-2312
    Keywords: actin ; bradykinin ; filamin ; phosphatase ; kinase ; permeability ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Endothelial cell (EC) cytoskeletal proteins are one of the earliest primary targets of second messenger cascades generated in response to inflammatory agonists. Actin binding proteins, by modulating actin gelation-solation state and membrane-cytoskeleton interactions, in part regulate cell motility and cell-cell apposition. This in turn can also modulate interendothelial junctional diameter and permeability. Nonmuscle filamin (ABP-280), a dimeric actincrosslinking protein, promotes orthogonal branching of F-actin and links microfilaments to membrane glycoproteins. In the present study, immunoblot analysis demonstrates that filamin protein levels are low in sparse EC cultures, increase once cell-cell contact is initiated and then decrease slightly at post-confluency. Both bradykinin and ionomycin cause filamin redistribution from the peripheral cell border to the cytosol of confluent EC. Forskolin, an activator of adenylate cyclase, blocks filamin translocation. Bradykinin activation of EC is not accompanied by significant proteolytic cleavage of filamin. Instead, intact filamin is recycled back to the membrane within 5-10 min of bradykinin stimulation. Inhibitors of calcium/calmodulin dependent protein kinase (KT-5926 and KN-62) attenuate bradykinin-induced filamin translocation. H-89, an inhibitor of cAMP-dependent protein kinase, causes translocation of filamin in unstimulated cells. Calyculin A, an inhibitor of protein phosphatases, also causes translocation of filamin in the absence of an inflammatory agent. ML-7, an inhibitor of myosin light chain kinase and phorbol myristate acetate, an activator of protein kinase C, do not cause filamin movement into the cytosol, indicating that these pathways do not modulate the translocation. Pharmacological data suggest that filamin translocation is initiated by the calcium/calmodulin-dependent protein kinase whereas the cAMP-dependent protein kinase pathway prevents translocation. Inflammatory agents therefore may increase vascular junctional permeability by increasing cytoplasmic calcium, which disassembles the microfilament dense peripheral band by releasing filamin from F-actin. © 1996 Wiley-Liss, Inc.
    Additional Material: 7 Ill.
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  • 7
    Electronic Resource
    Electronic Resource
    Replication of chromosomal and cytoplasmic DNA during mitosis and meiosis in the eucaryote Chlamydomonas reinhardiThis work was supported by National Science Foundation grants G-7025, G-15080, GB-3445, and GB-3064 (Princeton University), and by U. S. Public Health Service research grant FR-5367 (the University of Chicago). (1967)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 70 (1967), S. 89-112 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Both meiotic and mitotic replication of chromosomal and cytoplasmic DNAs in a unicellular green alga, Chlamydomonas reinhardi, have been studied by isotopic transfer experiments coupled with density-gradient centrifugation analysis. It has been shown that during the vegetative cycle (1) the replication mode of chloroplast DNA, as well as γ DNA, is semiconservative; (2) in synchronized culture these two cytoplasmic DNA satellites replicate coordinately with a high degree of synchrony; (3) the replication of chromosomal DNA is independent of and separable from that of the two cytoplasmic DNAs. It has been shown that during the sexual cycle (1) the chloroplast DNA replicates semiconservatively during zygote maturation, at which time there is neither chromosomal DNA replication nor nuclear division; (2) another DNA component (M-band DNA) replicates extensively and appears in large quantity; (3) meiosis occurs during zygote germination and is accompanied by one round of semiconservative chromosomal DNA replication; (4) the M-band DNA disappears in the early germination period, and the degraded substance is not incorporated into the newly replicated chromosomal DNA. A possible origin of the M-band DNA and a few properties of the cytoplasmic DNA satellites have been elucidated. Genetic recombination and regulation of incoordinate replication in an eucaryotic system have been discussed in terms of the data obtained.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040700408
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  • 8
    Electronic Resource
    Electronic Resource
    Methotrexate-poly(lysine) as a selective agent for mutants of chinese hamster ovary cells defective in endocytosis (1988)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 135 (1988), S. 277-284 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Methotrexate (MTX) covalently linked to poly(L-lysine) [poly(Lys)] enters cells by endocytosis, is degraded in lysosomes and, upon liberation of small molecular methotrexate, is cytocidal to Chinese hamster cells in culture. This drug conjugate was used to select mutants resistant to MTX-poly(Lys), which were examined for defects in endocytosis. Two mutants resistant to MYX-poly(Lys) and sensitive to free MTX, MPL 3-4 and MPL 2-5, internalized the conjugate in normal fashion, but had a decreased ability to degrade it to small molecular drug. The magnitude of this defect in the two mutants correlated with their level of resistance. In addition, both mutants were cross resistant to diphtheria toxin and modeccin and hypersensitive to ricin. While MPL 3-4 internalized MTX-poly(Lys) and inulin normally, it showed decreased endocytosis via the mannose-6-phosphate receptor and decreased uptake of 125I-alpha-2 macroglobulin. Acidification of subcellular fractions was measured using the partitioning of acridine orange. In MPL 3-4, the ATP-driven acidification of the endosome-containing cell fractions was slightly decreased (80% of controls), while acidification of the heavy lysosome-containing fraction was normal. Complementation analysis using hybrids of MPL 3-4 × MPL 2-5 indicated that the mutations occurred at the same gene, but were expressed with different severity. This genotype is identical to that of the End 2 mutants described by Roff et al. (1986). Thus, surprisingly, mutants with identical genotypes were isolated independently by totally different selection procedures.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041350215
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  • 9
    Electronic Resource
    Electronic Resource
    The cellular uptake of horseradish peroxidase and its poly(lysine) conjugate by cultured fibroblasts is qualitatively similar despite a 900-fold difference in rate (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 113 (1982), S. 167-178 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: When horseradish peroxidase (HRP) is conjugated to poly(L-lysine) of molecular weight (MW) 13,000, its transport into cultured L929 fibroblasts in 1 hour at 37°C is increased 918-fold. The kinetics of uptake are linear with time and concentration, reflecting a process of nonreceptor-mediated adsorptive endocytosis. Neither HRP-poly(Lys) conjugate nor free poly(Lys) of any size cause any increase in fluid phase endocytosis. All evidence indicates that the covalently bound poly(Lys) increases the binding of HRP to the cell surface and that this adequately accounts for an increased internalization occurring at a steady rate. In the absence of Ca++, both surface binding and uptake of the conjugate, but not of free HRP, are decreased. Cytochalasin B does not significantly inhibit the transport of either form of HRP. The half-lives of HRP and HRP-poly(Lys) are 7.6 and 5.6 hours, respectively, when measured over a period of 12 hours. Electron microscopic analysis of cells that have ingested comparable amounts of HRP shows that the intracellular localization of free and conjugated HRP are comparable and that both are found inside the same array of structures, thus HRP, which binds very poorly to the cell surface and is considered a fluid-phase marker for the “contents” of pinocytotic vesicles, and HRP-poly(Lys), which binds very strongly to the cell surface and is considered a membrane marker for adsorptive endocytosis, are taken up along the same endocytotic pathway. Moreover, despite the fact that neither markers are transported by receptor-mediated endocytosis, both are seen in structures that were described as receptosomes. It appears, therefore, that the pathways utilized in receptor-mediated transport are available to all other forms of pinocytosis.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041130126
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  • 10
    Electronic Resource
    Electronic Resource
    Altered endocytosis in a mutant of LM fibroblasts defective in cell-cell fusion (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 126 (1986), S. 161-166 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Mutants of LM fibroblasts selected for their decreased ability to undergo polyethylene glycol-induced cell-to-cell fusion (F40 subline) were examined for possible alterations of their ability to carry out endocytosis. Both fluidphase endocytosis of inulin and horseradish peroxidase and nonreceptor mediated adsorptive endocytosis of poly(L-lysine) were reduced to 60% of control values. Comparable results were obtained when the uptake of poly(L-lysine) was measured as internalization of surface-bound label in label-free medium or following continuous exposure. Accelerated breakdown of internalized label was ruled out as a cause for decreased label accumulation. Accelerated exocytosis is an unlikely cause, and it is suggested that the decreased uptake is due to a decrease in the constitutive membrane vesiculation process that leads to the formation of endocytotic vesicles. The capacity of F40 cells to degrade internalized horseradish peroxidase and poly(L-lysine) was not impaired, nor was their susceptibility to the cytotoxic action of methotrexate-poly(L-lysine). This drug conjugate must be degraded inside cells and release small molecular methotrexate in order to be cytocidal. These data suggest that only the first step of nonspecific endocytosis is impaired, while the subsequent steps that require fusion of endosomes to lysosomes proceed normally. Since the formation of primary endosomes requires membrane fusion through the external aspect of the plasma membrane and in that respect resembles cell-cell fusion, we propose the hypothesis that the observed decrease in endocytosis is related to the decreased ability of F40 cells to fuse with each other, and reflects a decreased efficiency of fusion processes at the external face of the plasma membrane.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041260203
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