Publication Date:
2011-05-21
Description:
Near the 5' end of most eukaryotic genes, nucleosomes form highly regular arrays that begin at canonical distances from the transcriptional start site. Determinants of this and other aspects of genomic nucleosome organization have been ascribed to statistical positioning, intrinsically DNA-encoded positioning, or some aspect of transcription initiation. Here, we provide evidence for a different explanation. Biochemical reconstitution of proper nucleosome positioning, spacing, and occupancy levels was achieved across the 5' ends of most yeast genes by adenosine triphosphate-dependent trans-acting factors. These transcription-independent activities override DNA-intrinsic positioning and maintain uniform spacing at the 5' ends of genes even at low nucleosome densities. Thus, an active, nonstatistical nucleosome packing mechanism creates chromatin organizing centers at the 5' ends of genes where important regulatory elements reside.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Zhang, Zhenhai -- Wippo, Christian J -- Wal, Megha -- Ward, Elissa -- Korber, Philipp -- Pugh, B Franklin -- HG004160/HG/NHGRI NIH HHS/ -- New York, N.Y. -- Science. 2011 May 20;332(6032):977-80. doi: 10.1126/science.1200508.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Center for Eukaryotic Gene Regulation, Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/21596991" target="_blank"〉PubMed〈/a〉
Keywords:
Adenosine Triphosphate/*metabolism
;
Base Sequence
;
Chromatin Assembly and Disassembly
;
Computational Biology
;
DNA, Fungal/chemistry/genetics
;
*Genes, Fungal
;
*Genome, Fungal
;
Histones/metabolism
;
Nucleosomes/*genetics/*metabolism
;
Poly dA-dT/analysis
;
Saccharomyces cerevisiae/genetics
;
Trans-Activators/genetics/metabolism
;
Transcription Initiation Site
;
Transcription, Genetic
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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