ISSN:
1432-2242
Keywords:
Key words Restriction fragment length polymorphism
;
Barley yellow dwarf
;
Resistance
;
Yd2
;
Morphological marker
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Barley yellow dwarf luteovirus (BYDV) causes serious yield losses in all cereals worldwide. The Yd2 gene from a number of Ethiopian barleys (Hordeum vulgare L.) has been the most effective means of providing resistance against BYDV in cultivated barley. Isolation of the Yd2 gene will enable characterisation of the molecular basis of the Yd2-BYDV interaction. This paper describes the first stage in a project to isolate the gene: the construction of a detailed linkage map of the Yd2 region. The map encompasses 27.6 centiMorgans (cM) of chromosome 3 and contains 19 RFLPs, 2 morphological marker loci, the centromere and Yd2. In the mapping population of 106 F2 individuals, Yd2 perfectly cosegregated with the RFLP loci Xwg889 and XYlp, which were located on the long arm, 0.5 cM from the centromere. The two morphological marker loci, uzu dwarf and white stripe,,j, both mapped distal to Yd2. The protein product of the gene at the XYlp locus will provide a convenient assay for the selection of Yd2 during the breeding of BYDV-resistant barley varieties.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s001220050203
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