ISSN:
1432-072X
Keywords:
Cellobiose
;
Cellodextrins
;
Anaerobic
;
Ethanol
;
β-Glucosidase
;
Bacteroides polypragmatus
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Bacteroides polypragmatus, a mesophilic obligate anaerobe, was shown to simultaneously ferment glucose and cellobiose giving ethanol as a major metabolic end-product. A mixture of higher cellodextrins was also utilized. The bacterium produced a β-glucosidase with a pI value of 4.2 and a molecular weight of approximately 100000 daltons. The enzyme was intracellular and functioned optimally at pH 7. The K m values obtained with p-nitrophenyl-β-d-glucoside and cellobiose as substrates were 0.73 mM and 100 mM, respectively. The enzyme was quite stable at elevated temperatures; in the presence of 10% glycerol (v/v), it had a half-life of 4 h at 55°C. It was also stable during long-term storage at either 4°C or-20°C, provided that 10% (v/v) glycerol was added to preparations maintained at-20°C.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00413033
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