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  • Bacteriophage MX-1  (2)
  • SPACE VEHICLES  (2)
  • Maize  (1)
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  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Archives of microbiology 108 (1976), S. 221-226 
    ISSN: 1432-072X
    Schlagwort(e): Bacteriophage MX-1 ; Myxococcus ; DNA ; Restriction fragments
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract 1. Bacteriophage MX-1 is a virulent DNA phage whose hosts include strains of Myxococcus xanthus, M. fulvus and M. virescens. DNA was extracted from purified phage preparations. The molecular weight of phage DNA was measured by sedimentation-velocity and by rate-zonal ultracentrifugation. The apparent molecular weight was found to vary for reasons discussed in the text. From ratezonal ultracentrifugation, using calibrated sucrose gradients, the molecular weight was calculated to be 149 (± 22)×106 daltons. The base composition of the DNA was estimated by different methods and was found to be 50–52% (G+C). The DNA demonstrated an anomalous thermal denaturation profile in dilute buffer. Denatured DNA was fractionated by ion-exchange chromatography and by buoyant-density centrifugation. No significant strand separation was obtained and it was concluded that overall base compositions of the two strands are very similar. 2. DNA from bacteriophage MX-1 was hydrolysed with restriction endonucleases R. EcoRI, R. EcoRII and R. HindIII. The restriction fragments were catalogued and their apparent molecular weights calculated from electrophoresis gels calibrated with fragments from the DNA of coliphage λ. From the total fragments obtained with nuclease R. EcoRI, the minimum apparent molecular weight of MX-1 DNA was found to be 130×106 daltons.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Archives of microbiology 108 (1976), S. 227-230 
    ISSN: 1432-072X
    Schlagwort(e): Bacteriophage MX-1 ; Myxococcus virescens ; Myxococcus xanthus ; Restriction ; Modification
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract 1. The plating efficiency of bacteriophage MX-1 on Myxococcus xanthus strains A and B and M. virescens V2 were compared. Comparison of strains V2 and A suggest that V2 is restrictive and A is not (restriction coefficient was approximately 8). A derivative of M. virescens V2 (strain V2-9) was obtained by repeated exposure of strain V2 to ultraviolet radiation. Strain V2-9 was also unrestrictive. Strain B is apparently unrestrictive too but analysis of phenotypic changes in phage derived from hosts V2, B and A suggested that some of the host-cell processes differ from orthodox restriction and modification. 2. Cell-free extracts from M. virescens V2 were fractionated by ion-exchange chromatography and two restriction endonucleases, R. MviV2I and R. MviV2II were identified. Nuclease I was found to hydrolyse coliphage λ DNA at apparently one site only and MX-1 DNA at approximately 10 sites; nuclease II was found to hydrolyse MX-1 DNA at a very large number of sites and its restriction sequence was of comparable frequency with that of R. EcoRII. “Modified MX-1 DNA”, obtained from phage whose last host was M. virescens V2 was hydrolysed by nuclease II but not by nuclease I. The significance of these findings for restriction in myxococci is discussed.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Springer
    Molecular genetics and genomics 208 (1987), S. 57-62 
    ISSN: 1617-4623
    Schlagwort(e): Transposable elements ; Mutation ; Mutator ; Maize
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The mutagenic activity of the maize transposable element system Mutator can be lost by outcrossing to standard, non-Mutator lines or by repetitive intercrossing of genetically diverse Mutator lines. Lines losing Mutator mutagenic activity in either manner retain high copy numbers (10–15 per diploid genome) of the Mutator-associated Mu transposable elements. Frequent transposition of Mu1-related elements is observed only in active Mutator lines, however. The loss of Mutator activity on intercrossing is correlated with an increase in the copy number of Mu1-like elements to 40–50 per diploid genome, implying a self-encoded or self-activated negative regulator of Mu1 transposition. The outcross loss of Mutator activity is only weakly correlated with a low Mu element copy number and may be due to the loss of a positive regulatory factor encoded by a subset of Mu1-like elements. Transposition of Mu elements in active Mutator lines generates multiple new genomic positions for about half the elements each plant generation. The appearance of Mu1-like elements in these new positions is not accompanied by equally high germinal reversion frequencies, suggesting that Mu1 may commonly transpose via a DNA replicative process.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    facet.materialart.
    Unbekannt
    In:  Other Sources
    Publikationsdatum: 2019-07-13
    Beschreibung: The orbiter ECLSS (Environmental Control and Life Support System) provides the functions of atmosphere revitalization, crew life support, and active thermal control. This paper describes these functions as they relate to the support of Shuttle payloads, including automated spacecraft, Spacelab and Department of Defense missions. Functional and performance requirements for the orbiter ECLSS which affect payload support are presented for the atmosphere revitalization subsystem, the food, water and waste subsystem, and the active thermal control subsystem. Schematics for these subsystems are also described. Finally, based on the selected orbiter configuration, preliminary design and off-design thermodynamic data are presented to quantify the baseline orbiter capability; to quantify the payload chargeable penalties for increasing this support; and to identify the significant limits of orbiter ECLSS support available to Shuttle payloads.
    Schlagwort(e): SPACE VEHICLES
    Materialart: ASME PAPER 74-ENAS-22 , Intersociety Conference on Environmental Systems; Jul 29, 1974 - Aug 01, 1974; Seattle, WA; US
    Format: text
    Standort Signatur Erwartet Verfügbarkeit
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  • 5
    Publikationsdatum: 2019-07-13
    Beschreibung: Previous spacecraft heat rejection systems have been tailored to a specific spacecraft and a specific mission. Many different panel designs, fluids, and control methods have been used with no significant degree of commonality. For future spacecraft, it is desirable to have heat rejection systems which can accommodate widely varying loads and have a high degree of commonality to minimize development requirements. A modular radiator system which satisfies this requirement and is adaptable to a variety of future vehicles and missions is described. A test program with two modular panels connected in several flow arrangements, which are representative of candidate shuttle orbiter and other future spacecraft applications, is described.
    Schlagwort(e): SPACE VEHICLES
    Materialart: ASME PAPER 72-ENAV-34 , American Society of Mechanical Engineers, Environmental Control and Life Support Systems Conference; Aug 14, 1972 - Aug 16, 1972; San Francisco, CA
    Format: text
    Standort Signatur Erwartet Verfügbarkeit
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